Publications by authors named "Xue Jiang Zhu"
Detection of antibodies against customized epitope: use of a coating antigen employing VEGF as fusion partner.
Appl Microbiol Biotechnol
August 2014
Article Synopsis
- The study focused on using VEGF121 as a carrier for the small peptide GnRH to create a platform for detecting specific antibodies, overcoming challenges in coating small peptides in ELISA.
- The fusion protein VEGF-GnRH was effectively produced in E. coli and purified, yielding about 235 mg from a 2.1 L culture.
- ELISA and western blot tests demonstrated that VEGF-GnRH could successfully detect anti-GnRH antibodies in serum, indicating that VEGF121 can serve as a versatile partner for various diagnostic peptide markers.
To define the molecular basis of ethanol dependence, changes in the phosphorylation of cAMP response element binding protein (CREB) in the nucleus accumbens of rats after acute and chronic ethanol administration were detected using immunohistochemistry. The results demonstrate that the expression of phospho-CREB (p-CREB) protein in the rat nucleus accumbens significantly increased after 15 min of acute ethanol exposure, reaching a peak at 30 min after ethanol administration. The increment remained after 1 or 6 h of ethanol exposure compared to the control rats.
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