Publications by authors named "Xudan Tian"
Article Synopsis
- Biotechnologists are exploring co-transformation methods to develop marker-free transgenic plants, focusing on how Agrobacterium strain ratios and selection schemes affect the frequency of successful transformations.
- The study tested various combinations of fluorescent markers and selectable vectors in rice, finding that the pGFP-HPT/pRFP combination yielded higher co-transformation rates (70.9% to 81.2%) compared to the pGFP-HPT/pRFP-HPT combination, which averaged 56.6%.
- Results indicate that about 55% of the successful co-transformants had unlinked T-DNAs and showed distinct segregation patterns, highlighting how both the vector system and selection method play crucial roles in co-transformation efficiency
Rice blast is one of the most serious diseases of rice and a major threat to rice production. Breeding disease-resistant rice is one of the most economical, safe, and effective measures for the control of rice blast. As a complement to traditional crop breeding, the transgenic method can avoid the time-consuming process of crosses and multi-generation selection.
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