Target genes regulated by CLEC16A intronic region associated with common variable immunodeficiency.

Background: CLEC16A intron 19 has been identified as a candidate locus for common variable immunodeficiency (CVID).

Objectives: This study sought to elucidate the molecular mechanism by which variants at the CLEC16A intronic locus may contribute to the pathogenesis of CVID.

Methods: The investigators performed fine-mapping of the CLEC16A locus in a CVID cohort, then deleted the candidate functional SNP in T-cell lines by the CRISPR-Cas9 technique and conducted RNA-sequencing to identify target gene(s).

Fermentation-mediated variations in structure and biological activity of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg.

Variations in the structure and activities of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg fermented by Sanghuangporus sanghuang fungi were investigated. Compare with the unfermented polysaccharide (THDP2), the major monosaccharide composition and molecular weight of polysaccharide after fermentation (F-THDP2) altered dramatically, which caused galactose-induced conversion from glucose and one-third of molecular weight. F-THDP2 had a molecular weight of 1.

Molecular Characterization and Bioactivities of a Novel Polysaccharide from Bamboo Shoot Residues.

Dietary carbohydrates are unexploited in the by-products of economically valuable bamboo shoots. A residue-derived polysaccharide (PBSR1) was aqueously extracted from the processing waste of this bamboo shoot species. Its primary structure and advanced conformation were elucidated by a combined analysis of spectroscopy, chromatography, 2D nuclear magnetic resonance, laser light scattering and atomic microscopy.

Enhanced anaerobic hydrogen production from cotton straws assisted by copper molybdate.

Hydrogen production from dark fermentation has potential application due to its environmental friendliness, low production cost, and sustainability. However, there is still an obstacle to improving the efficiency of bioH production to meet the requirements in practical applications. In this research, copper molybdates are synthesized under different pH conditions as additives to study their different influence processes during anaerobic hydrogen production from cotton straws with the pure cultural system.

Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated under forest.

A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that SVPS2 was a kind of 1, 5-linked β-Glucofurannan consisting of β-glucose, β-galactose and α-fucose with 23.

Ni(NH) more efficient than Ni(HO) and Ni(OH) for catalyzing water and phenol oxidation on illuminated BiMoO with visible light.

Nickel (hydr)oxide (NiOH) is known to be good co-catalyst for the photoelectrochemical oxidation of water, and for the photocatalytic oxidation of organics on different semiconductors. Herein we report a greatly improved activity of BiMoO (BMO) by nickel hexammine perchlorate (NiNH). Under visible light, phenol oxidation on BMO was slow.

Conformation and anticancer activity of a novel mannogalactan from the fruiting bodies of Sanghuangporus sanghuang on HepG2 cells.

A novel water-soluble mannogalactan (SSPS1) with an average molecular weight of 2.04 × 10 Da was obtained from the fruiting bodies of the Sanghuangporus sanghuang. It revealed that SSPS1 was composed of d-galactose, d-mannose, l-fucose, 3-O-methylgalactose and d-glucose in a ratio of 6.

Inhibitory mechanism of two homoisoflavonoids from on tyrosinase activity: insight from spectroscopic analysis and molecular docking.

The inhibition mechanism of two homoisoflavonoids from including methylophiopogonanone A (MO-A) and methylophiopogonanone B (MO-B) on tyrosinase (Tyr) was studied by multiple spectroscopic techniques and molecular docking. The results showed that the two homoisoflavonoids both inhibited Tyr activity a reversible mixed-inhibition, with a half inhibitory concentration (IC) of (10.87 ± 0.

Tumor Mutational Burden Associated With Response to Hyperthermic Intraperitoneal Chemotherapy.

Background: Gastric cancer (GC) is one of the most common cancer types, especially in Asian countries. Hyperthermic intraperitoneal chemotherapy (HIPEC) has been shown to improve the progression-free survival among gastric cancer patients with peritoneal metastases; however, not all patients demonstrate response to HIPEC.

Methods: Biomarkers are needed to select patients for effective treatment of HIPEC.

Amorphous NiS and FeOOH as bifunctional co-catalysts for oxygen reduction and phenol (water) oxidation over BiVO under visible light.

Monoclinic BiVO (BiV) has been widely used as a photoanode for water oxidation, but rarely as a photocatalyst for organic oxidation due to slow reaction of O. In this work, BiV has been modified with poorly crystallized sFe and sNi, where sFe is FeOOH, and sNi is a mixture of Ni(OH) and polysulfide. Under light, sFe/BiV and sNi/BiV in aqueous solution were more active than BiV, respectively, not only for phenol oxidation but also for O reduction.

CuCoOx/BiVO multifunctional catalyst for organics degradation, water oxidation, and O reduction under visible light.

Monoclinic BiVO (BiV) is an excellent photoanode for water oxidation, but it is a poor photocatalyst for organic oxidation due to slow O reduction. Herein we report a 94-fold increased photocatalytic activity of BiV through a surface deposited CuCoOx. The model reaction was phenol degradation in aqueous solution under visible light.

Fast organic degradation on Ti- and Bi-based photocatalysts via co-deposited Pt and Ni(PO) nanoparticles.

Environmental remediation via semiconductor (SC) photocatalysis has attracted great attention over the past three decades. However, prospect for large scale application is still under debate, basically due to the bottleneck of fast charge recombination and/or slow surface reaction. Herein we report a universal solution of speeding up organic degradation simply via co-deposited Pt and nickel phosphate (NiP).

The Role of Autophagy in Skeletal Muscle Diseases.

Skeletal muscle is the most abundant type of tissue in human body, being involved in diverse activities and maintaining a finely tuned metabolic balance. Autophagy, characterized by the autophagosome-lysosome system with the involvement of evolutionarily conserved autophagy-related genes, is an important catabolic process and plays an essential role in energy generation and consumption, as well as substance turnover processes in skeletal muscles. Autophagy in skeletal muscles is finely tuned under the tight regulation of diverse signaling pathways, and the autophagy pathway has cross-talk with other pathways to form feedback loops under physiological conditions and metabolic stress.

Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.

A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of d-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.

Improved Performance of BiVO via Surface-Deposited Magnetic CuFeO for Phenol Oxidation and O Reduction and Evolution under Visible Light.

Monoclinic BiVO (BiV) is a good photoanode for water oxidation but a poor photocatalyst for organic oxidation because of slow O reduction. In this work, a well-crystallized BiV microdecahedron (1-2 μm) has been deposited with a poorly crystallized cubic CuFeO (CF) nanosphere (100 nm). For phenol oxidation and O reduction to HO in aqueous suspension under visible light, 1 wt % CF/BiV was more active than BiV by approximately factors of 14 and 7, respectively, while CF was almost not active.