A comprehensive physiological and -Omic analysis of trypsin-mediated protection of green pepper fruits from chilling injury.

Chilling injury (CI) in green pepper fruits during low-temperature storage causes a significant decline in quality. The present study utilized physiological, transcriptomic, and metabolomic analyses to idneitfy the mechanisms by which trypsin mitigates CI in green peppers stored at 4 °C for 8 days, followed by 3 days of shelf life. Results indicated that the trypsin treatment significantly reduced electrolyte leakage and the CI index in peppers, effectively extending their shelf life and preserving postharvest quality.

Classification and transfer learning of sleep spindles based on convolutional neural networks.

Background: Sleep plays a critical role in human physiological and psychological health, and electroencephalography (EEG), an effective sleep-monitoring method, is of great importance in revealing sleep characteristics and aiding the diagnosis of sleep disorders. Sleep spindles, which are a typical phenomenon in EEG, hold importance in sleep science.

Methods: This paper proposes a novel convolutional neural network (CNN) model to classify sleep spindles.

A Wireless, High-Quality, Soft and Portable Wrist-Worn System for sEMG Signal Detection.

The study of wearable systems based on surface electromyography (sEMG) signals has attracted widespread attention and plays an important role in human-computer interaction, physiological state monitoring, and other fields. Traditional sEMG signal acquisition systems are primarily targeted at body parts that are not in line with daily wearing habits, such as the arms, legs, and face. In addition, some systems rely on wired connections, which impacts their flexibility and user-friendliness.

Effect of miR-499-5p/SOX6 axis on atrial fibrosis in rats with atrial fibrillation.

Atrial fibrosis is involved in the progression of atrial fibrillation (AF). miR-499-5p is the most downregulated microRNA in arrhythmogenic cardiomyopathy hearts. Sry-related high-mobility-group box 6 (SOX6) is associated with apoptosis, inflammatory responses, and fibrosis.

Optically modulated ionic conductivity in a hydrogel for emulating synaptic functions.

Ion-conductive hydrogels, with ions as signal carriers, have become promising candidates to construct functional ionotronics for sensing, actuating, and robotics engineering. However, rational modulation of ionic migration to mimic biological information processing, including learning and memory, remains challenging to be realized in hydrogel materials. Here, we develop a hybrid hydrogel with optically modulated ionic conductivity to emulate the functions of a biological synapse.

Reconfigurable Touch Panel Based on a Conductive Thixotropic Supramolecular Hydrogel.

Touch panels based on ionic conductive hydrogels perform excellent flexibility and biocompatibility, becoming promising candidates for the next-generation human-machine interface. However, these ionic hydrogels are usually composed of cross-linked polymeric networks that are difficult to be recycled or reconfigured, resulting in environmental issues. Herein, we designed a lithium ion-triggered gelation strategy to provide a conductive molecular hydrogel with thixotropy, which can be mechanically recycled or reconfigured at room temperature.

HDAC3 Impairs Cardiac Function in Chronic Heart Failure Rats Mediating MicroRNA-26b-3p to Target High Mobility Group AT-Hook 2.

Background: Histone deacetylase 3 (HDAC3) has been studied in chronic heart failure (CHF), while the regulatory mechanism of HDAC3 on the development of CHF in regulating microRNA (miR)-26b-3p/high mobility group AT-hook 2 (HMGA2) axis has not been extensively investigated. This study aimed to probe the effects of HDAC3, miR-26b-3p and HMGA2 on CHF.

Methods: CHF rat models were established using aortic coarctation.

Safety and anterior chamber structure of evolution implantable Collamer lens implantation with short white-to-white corneal diameters.

Introduction: To evaluate the safety and anterior chamber structure of implantation of the Evolution (EVO) implantable Collamer lens (EVO-ICL) in patients with short white-to-white (WTW) corneal diameters.

Materials And Methods: The study population was divided into two groups: the experimental group (34 eyes of 34 patients) with WTW corneal diameters of ≤10.6 mm and the control group (59 eyes of 59 patients) with WTW corneal diameters of >10.

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells.

Background And Objectives: This study aims to clarify the systems underlying regulation and regulatory roles of hydrogen combined with 5-Aza in the myogenic differentiation of adipose mesenchymal stem cells (ADSCs).

Methods And Results: In this study, ADSCs acted as an in vitro myogenic differentiating mode. First, the Alamar blue Staining and mitochondrial tracer technique were used to verify whether hydrogen combined with 5-Aza could promote cell proliferation.

One-year analysis of the refractive stability, axial elongation and related factors in a high myopia population after Implantable Collamer Lens implantation.

Purpose: To investigate the refractive stability, axial length (AL) changes and their related factors in a high myopia population after Implantable Collamer Lens (ICL) implantation.

Methods: This prospective study included 116 eyes of 116 patients divided into several groups based on the spherical equivalent refractive error (SE)-SE > - 6 D, - 12 ≤ SE < - 6 D and SE < - 12 D groups-and AL-AL < 28 mm and AL ≥ 28 mm groups. The uncorrected and corrected distance visual acuity, refraction, AL and intraocular pressure were followed for 1 year.

miR-29b ameliorates atrial fibrosis in rats with atrial fibrillation by targeting TGFβRΙ and inhibiting the activation of Smad-2/3 pathway.

Objective: Atrial fibrillation (AF) is a major cause of stroke with lifetime risks. microRNAs (miRNAs) are associated with AF attenuation, yet the mechanism remains unknown. This study investigated the functional mechanism of miR-29b in atrial fibrosis in AF.

Safety of intraocular pressure measurement using air-puff tonometer after implantable collamer lens implantation.

Purpose: To determine the safety of intraocular pressure (IOP) measurement using a noncontact tonometer in patients who have undergone implantable collamer lens (ICL) implantation.

Setting: Eye, Ear, Nose, and Throat Hospital.

Design: Prospective study.

Bilateral Differential Topography-A Novel Topographic Algorithm for Keratoconus and Ectatic Disease Screening.

The purpose of this study was to establish a novel bilateral differential topographic algorithm and assess its efficacy for screening of keratoconus and corneal ectasia before corneal refractive surgery. One hundred and sixty-one consecutive patients (115 men and 46 women, aged 22.8 ± 6.

Long-term Comparison of Vault and Complications of Implantable Collamer Lens with and without a Central Hole for High Myopia Correction: 5 Years.

Purpose: To investigate the long-term safety, efficacy, stability, vault, and complications of implantable collamer lens with (ICL V4c) and without (ICL V4) a central hole for correcting high myopia.

Methods: 78 eyes (40 patients) underwent ICL V4c implantation and 78 eyes (48 patients) underwent ICL V4 implantation were enrolled. They were followed up for 5 years of the uncorrected and corrected distance visual acuity, spherical equivalent (SE), axial length, intraocular pressure, endothelial cell density and vault.

Five-year outcomes of EVO implantable collamer lens implantation for the correction of high myopia and super high myopia.

Background: To evaluate the long-term safety, efficacy, predictability, and stability of implantable collamer lens with a central hole (EVO ICL) implantation for correcting high myopia (HM) and super high myopia (SHM).

Methods: This prospective study evaluated 83 eyes of 46 patients who were divided into groups based on their spherical equivalent refractive error (SE): HM group (- 12 D ≤ SE < - 6 D) and SHM group (SE < - 12 D). They were followed up for 5 years after ICL implantation; assessments of uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), manifest refractive error, axial length, intraocular pressure, corneal endothelial cell density, and vault were conducted, and a questionnaire was administered.

Effect of the Difference Between the White-to-White and Sulcus-to-Sulcus on Vault and the Related Factors After ICL Implantation.

Introduction: To assess the differences between the horizontal white-to-white (WTW) and horizontal sulcus-to-sulcus (STS) diameter measurements, their related factors, and their effects on vault after implantable Collamer lens (ICL) implantation.

Methods: This retrospective study included 429 eyes of 429 patients (145 men and 284 women with a mean age of 29.22 ± 8.

Identification of key pathways and hub genes in the myogenic differentiation of pluripotent stem cell: a bioinformatics and experimental study.

Background: The regeneration of muscle cells from stem cells is an intricate process, and various genes are included in the process such as myoD, mf5, mf6, etc. The key genes and pathways in the differentiating stages are various. Therefore, the differential expression of key genes after 4 weeks of differentiation were investigated in our study.

Conserved DNA sequences discovered from atrial fibrillation related genes.

Many genes change their transcription in atrial fibrillation (AF) patients, which indicates the functional relationship of these genes with AF. Thus, identifying the over-represented motifs in the upstream region of these genes would shed light on the mechanism of this disease. We analyzed a set of microarray experiments, selected from genes whose expression was changed, and extracted their regulatory sequences from genome databases.