[Characteristics of FTIR spectra of biogenic aragonite in mollusk shells].

The FTIR spectra of aragonites in outer and inner layers of thirteen species of mollusk shells were systematically measured, and the frequency variations of frequency 1, frequency 2 and frequency 4 bands of the aragonites with the positions of shell layers were analyzed. The results showed that the frequency of v2 band differed in different species of shells and the positions of shell layers, but the frequencies of the other two bands were not altered, and had the same values with synthetic aragonites. In the same species of shells, the frequency of frequency 2 band in inner layers was greater than that in outer layers, and the frequency shift of frequency 2 band between inner and outer layers was in the range of 0.

Effects of dietary supplementation with vitamin E and selenium on rat hepatic stellate cell apoptosis.

Aim: To evaluate the effects of dietary supplementation with vitamin E and selenium on proliferation and apoptosis of hepatic stellate cells (HSCs), in acute liver injury induced by CCl(4), and to explore their role in the recovery from hepatic fibrosis phase.

Methods: An acute liver damage model of rats was established by intraperitoneal injection of carbon tetrachloride (0.3 mL/100 g body weight) twice a week, then the rats were killed at 6, 24, 48, and 72 h after the first and third injection, respectively.

[Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis].

Objective: Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4.

Methods: Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry.

Result: During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little.