Functional outcomes and quality of life in patients with osteosarcoma treated with amputation versus limb-salvage surgery: a systematic review and meta-analysis.

Introduction: To perform a meta-analysis for comparing the functional outcomes and quality of life (QOL) of osteosarcoma patients receiving amputation or limb-salvage surgeries.

Materials And Methods: A search was conducted of the Medline, Cochrane, EMBASE, and Google Scholar on September 30, 2013. Studies were included in the analysis if there were patients who underwent amputation and limb-salvage surgery for osteosarcoma or Ewing's sarcoma, and for whom postoperative functional outcomes and QOL were evaluated.

The role of VE-cadherin in osteosarcoma cells.

Osteosarcoma cells can generate vasculogenic-like, patterned networks to obtain nutrients and oxygen, which mimic some function of endothelial-like cells and facilitate tumor malignant progress. These cells also express vascular endothelial-cadherin (VE-cadherin), which is generally accepted as a strictly endothelial-specific transmembrane protein. However, its role is still relatively obscure in osteosarcoma cells.

[Morphological changes in osteosarcoma xenografts in nude mice after inhibiting angiogenesis by Ad-VEGF-siRNA].

Background And Objective: Vascular endothelial growth factor (VEGF) is a critical stimulator for angiogenesis of osteosarcoma. This study was to investigate the morphological changes in osteosarcoma xenografts in nude mice after inhibiting angiogenesis by Ad-VEGF-siRNA.

Methods: The animal model of osteosarcoma xenografts was constructed by subcutaneous inoculation of osteosarcoma MG63 cells into nude mice.

Inhibitory effects of VEGF-siRNA mediated by adenovirus on osteosarcoma-bearing nude mice.

As one of the blood-rich malignancies, the growth and metastasis of osteosarcoma both depend on its angiogenesis, a procedure in which vascular endothelial growth factor (VEGF) acts essentially. Although with the advent of neoadjuvant chemotherapy, more aggressive surgical excision and logical therapy strategy, the 5-year survival rate remains relatively stable at 70%, at best. However, antiangiogenic therapeutics, through gene silencing and targeting key sequences, probably brings an outlook to the conventional algorithm.

[Effect of EPHA2-siRNA plasmid on biological behavior of human osteosarcoma cells in vitro].

Objective: To investigate the role of EPHA2 in regulating apoptosis, proliferation and vasculogenic mimicry of osteosarcoma cells, by gene silencing through RNA interference.

Methods: EPHA2-siRNA plasmids were achieved by gene cloning. The plasmids were transfected into human osteosarcoma cells (MG63).

Tumor blood vessels formation in osteosarcoma: vasculogenesis mimicry.

Background: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma.

Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen.