Insights into the mA demethylases FTO and ALKBH5 : structural, biological function, and inhibitor development.

N6-methyladenosine (mA) is dynamically regulated by methyltransferases (termed "writers") and demethylases (referred to as "erasers"), facilitating a reversible modulation. Changes in mA levels significantly influence cellular functions, such as RNA export from the nucleus, mRNA metabolism, protein synthesis, and RNA splicing. They are intricately associated with a spectrum of pathologies.

Insight into the regulatory mechanism of mA modification: From MAFLD to hepatocellular carcinoma.

In recent years, there has been a significant increase in the incidence of metabolic-associated fatty liver disease (MAFLD), which has been attributed to the increasing prevalence of type 2 diabetes mellitus (T2DM) and obesity. MAFLD affects more than one-third of adults worldwide, making it the most prevalent liver disease globally. Moreover, MAFLD is considered a significant risk factor for hepatocellular carcinoma (HCC), with MAFLD-related HCC cases increasing.

Multi-stage virtual screening of natural products against p38α mitogen-activated protein kinase: predictive modeling by machine learning, docking study and molecular dynamics simulation.

p38α is a mitogen-activated protein kinase (MAPK), and the signaling pathways involved are closely related to the inflammation, apoptosis and differentiation of cells, which also makes it an attractive target for drug discovery. With the high efficiency and low cost, virtual screening technology is becoming an indispensable part of drug development. In this study, a novel multi-stage virtual screening method based on machine learning, molecular docking and molecular dynamics simulation was developed to identify p38α MAPK inhibitors from natural products in ZINC database, which improves the prediction accuracy by considering and utilizing both ligand and receptor information compared to any individual approach.

Mixture Compound Fertilizer and Super Absorbent Polymer Application Significantly Promoted Growth and Increased Nutrient Levels in Seedlings and Soil in Seriously Eroded Degradation Region of Southern China.

is the pioneer tree species in the red soil regions of southern China, however, the serious understory soil erosion and nutrient deficiency in that region are the main factors restricting the growth of This field study examined the effects of compound fertilizer and super absorbent polymer (SAP) on the physiology, growth characteristics, biomass, soil nutrient, plant nutrient content, and nutrient uptake efficiency of 1-year-old seedlings for 2 years at Changting, Fujian in South China. One control (no fertilizer, CK) and fertilization treatments were established, namely, single compound fertilizer application (0.94, 1.

The role of N6-methyladenosine mRNA in the tumor microenvironment.

In recent years, the most widely distributed eukaryotic messenger RNA (mRNA) modification, N6-methyladenosine (m6A), has received a large amount of interest, in part due to the development and advances of high-throughput RNA sequencing. The effects of m6A mRNA on tumor progression have been the most widely studied, and large amounts of conflicting data have been reported due to differences in tumor contexts, cell types or cell states. The majority of these studies were related to the significance of m6A mRNA on tumor cells, including on proliferation, stemness, invasion capability, etc.

Assessing spatial variability of soil organic carbon and total nitrogen in eroded hilly region of subtropical China.

The hilly red soil region of southern China suffers from severe soil erosion that has led to soil degradation and loss of soil nutrients. Estimating the content and spatial variability of soil organic carbon (SOC) and soil total nitrogen (STN) and assessing the influence of topography and land-use type on SOC and STN after years of soil erosion control are important for vegetation restoration and ecological reconstruction. A total of 375 topsoil samples were collected from Changting County, and their SOC and STN distributions were studied by using descriptive statistics and geostatistical methods.

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Improving the microenvironment of lesioned spinal cord to minimize the secondary injury is one important strategy to treat spinal cord injury (SCI). The ensuing hemorrhage after SCI has tight connection with ferroptosis. This study investigated the effects of proanthocyanidins (PACs) on SCI repair and the underlying mechanisms.

[Soil stoichiometry of Pinus massoniana forest in red soil erosion area under different management patterns].

We investigated the concentrations of carbon (C), nitrogen (N), and phosphorus (P) and C:N:P stoichiometry in soil and litter of Pinus massoniana forest under four different management patterns: inefficient forest transformation, mixture of arbor with shrub and herb, shallow ditch grass planting, and being banned in serious erosion and degradation of red soil in southern China. Our findings could provide scientific basis for soil erosion control and vegetation restoration in this area. The results showed that there were significant differences in soil organic carbon (SOC), TN, TP and litter nutrient content among different management patterns.

Effects of the ENSO on rainfall erosivity in the Fujian Province of southeast China.

Rainfall erosivity is one important factor that controls soil erosion. The interannual variability of rainfall erosivity in southeast China connected to the ENSO (El Niño-Southern Oscillation). Rainfall erosivity across southeast China was assessed using daily rainfall data from 60 meteorological stations during the period from 1980 to 2013.

Amentoflavone enhances osteogenesis of human mesenchymal stem cells through JNK and p38 MAPK pathways.

Amentoflavone is a bioflavonoid found in a variety of traditional Chinese medicines such as Gingko and Selaginella tamariscina. It has been reported that amentoflavone has anti-inflammatory, antioxidant, antiviral and anticancer effects. However, the effect of amentoflavone on osteogenic differentiation of human mesenchymal stem cells (hMSCs) has not been studied.

Apigenin promotes osteogenic differentiation of human mesenchymal stem cells through JNK and p38 MAPK pathways.

Apigenin is a plant-derived flavonoid and has been reported to prevent bone loss in ovariectomized mice, but the role of apigenin on osteogenic differentiation of human mesenchymal stem cells (hMSCs) has not been reported. In the present study, the effect of apigenin on osteogenic differentiation of hMSCs was explored. Our results showed that apigenin treatment significantly increased alkaline phosphatase (ALP) activity and mineralization in hMSCs.