The characteristic of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1 alleles in Zhejiang Han population.

The Zhejiang Han population, a subgroup of the Southern Han ethnic group, resides in Zhejiang Province, situated on the southeast coast of China. In this study, we conducted HLA genotyping for 813 voluntary umbilical cord blood donors from the Zhejiang Han population, targeting 11 HLA loci, namely HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1, using the next-generation sequencing method. Our analysis of the alleles and haplotypes revealed a high degree of polymorphism within these loci.

Determination for KIR genotype and allele copy number via real-time quantitative PCR method.

Killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a real-time quantitative PCR (qPCR) to genotype all KIR genes and their copy numbers simultaneously. With 18 pairs of locus-specific primers, we identified KIR genes by Ct values and determined KIR copy number using the 2 method.

Association of GAB1 gene with asthma susceptibility and the efficacy of inhaled corticosteroids in children.

Asthma is a polygenic disease that may onset during childhood. Inhaled corticosteroids (ICS) are the main therapy in asthma, although their efficacy varies among individuals. Nuclear factor κB (NF-κB) is an important target of ICS treatment of asthma.

Significant CircRNAs in liver cancer stem cell exosomes: mediator of malignant propagation in liver cancer?

Hepatocellular carcinoma (HCC), one of the most prevalent forms of cancer worldwide, presents a significant global healthcare challenge. Cancer stem cells (CSCs), which can influence neighboring non-CSCs, are believed to play a crucial role in tumor growth and resistance to treatment, but the specific mechanisms and mediators are not fully understood. Regulation of the CSC state is considered an ideal therapeutic strategy both in the early stages of tumor formation and within established tumors.

Identification of the novel KIR3DP1*00604 allele in a Chinese individual.

KIR3DP1*00604 differs from KIR3DP1*0060101 by one single nucleotide substitution G > C at position 252.

The novel allele, HLA-B*15:627 was identified by next-generation sequencing in a Chinese cord blood donor.

HLA-B*15:627 shows one nucleotide substitution G to A at position 601 compared with HLA-B*15:02:01:01.

Characterization of the novel HLA-C*01:225 allele in a Chinese individual.

HLA-C*01:225 has one nucleotide change compared with HLA-C*01:02:01:01 in codon 110 of exon 3.

The novel HLA-DRB1*14:222N allele was identified by next-generation sequencing.

HLA-DRB1*14:222N differs from HLA-DRB1*14:03:01 by one single nucleotide substitution at position 262.

The novel HLA-A*26:174 allele was identified in a Chinese individual.

HLA-A*26:174 shows a single nucleotide substitution at position 148 G>T when compared to HLA-A*26:01:01:01.

Characterization of the novel HLA-C*01:02:56 and HLA-C*01:02:57 alleles by sequencing-based typing.

Compared with HLA-C*01:02:01:01, the alleles HLA-C*01:02:56 and HLA-C*01:02:57 each show one synonymous nucleotide substitution.

Characterization of the novel HLA allele: HLA-B*15:437 in a Chinese bone marrow donor.

HLA-B*15:437 shows one nucleotide difference with B*15:02 at position 814 G>A.

Identification of two novel HLA-C alleles, HLA-C*07:02:92 and HLA-C*07:828 in Chinese individuals.

Two novel HLA-C alleles, HLA-C*07:02:92 and HLA-C*07:828, were identified.

Characterization of the novel HLA-C*01:154 allele by polymerase chain reaction sequencing-based typing.

HLA-C*01:154 has a single nucleotide substitution at position 312 C > A when compared with HLA-C*01:02.

Characterization of the novel HLA-A*11:280 allele by next-generation sequencing in a Chinese cord blood donor.

HLA-A*11:280 differs from HLA-A*11: 01: 01: 01 by a single nucleotide substitution at position 982 G>T.

Identification of the novel HLA-C*12:220 allele in a Chinese individual.

The new allele HLA-C*12:220 differs from HLA-C*12:03:01:01 by one nucleotide substitution in exon 7.