A highly sensitive TaqMan real-time PCR assay for early detection of Schistosoma species.

Schistosomiasis is a major infectious disease and a public health concern in many areas in China and other countries. Sensitive method for detection of the parasite is critical for early diagnosis and for monitoring of effective treatment of the disease. In this study, we developed a highly sensitive TaqMan real-time PCR assay for the detection of Schistosoma japonicum DNA in mouse feces and serum samples.

Two new compounds from an endophytic fungus Aspergillus sp. HS-05.

Two new compounds, N-[4'-hydroxy-(E)-cinnamoyl]-l-tyrosine methyl ester (1) and methyl 4-methoxy-3-(3'-hydroxy-2'-methyl)propionyloxy-benzoate (2), were isolated from EtOAc extract of the fermentation broth of the endophytic fungus Aspergillus sp. HS-05. Their structures were elucidated by NMR, IR, UV, MS, and CD methods.

[Medicinal cupping: clinical application].

The commonly used tools and key points of manipulations of medicinal cupping therapy were introduced systematically in this article in order to study the concerning apparatuses, therapeutic methods, detailed manipulations, indications, application locations and medicine selection. Thus, an overall comprehension of the clinical application of medicinal cupping can be acquired, which may provide train of thought and methodology for its clinical application.

Two new steroidal saponins from Tribulus terrestris L.

Two new steroidal saponins were isolated from the fruits of Tribulus terrestris L. Their structures were elucidated by spectroscopic and chemical analysis as (23S,24R,25R)-5alpha-spirostane-3beta,23,24-triol-3-O-{alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-glucopyranosyl-(1 --> 4)]-beta-D-galactopyranoside} (1) and (23S,24R,25S)-5alpha-spirostane-3beta,23,24-triol-3-O-{alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-glucopyranosyl-(1 --> 4)]-beta-D-galactopyranoside} (2).

Two new compounds from the broth of the marine fungus Penicillium griseofulvum Y19-07.

Two new compounds, 4-hydroxyphenethyl methyl succinate (1) and 4-hydroxyphenethyl 2-(4-hydroxyphenyl)acetate (2), were isolated from the EtOAc extract of the broth of the marine fungus Penicillium griseofulvum Y19-07. Five known compounds were also obtained in this study. The structures of the new compounds were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry.

Molecular cloning and characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloid production in AaTRI-transformed hairy roots.

Tropane alkaloids are used medicinally as anticholinergic agents with increasing market demand, so the improvement and production of active components from medicinal plants using molecular biotechnology show great potential for applications that should benefit human healthcare. Two tropinone reductases constitute a branching point in the biosynthesis of tropane alkaloids. In the present paper, we report for the first time the cloning and characterization of two fulllength cDNAs encoding TRI (tropinone reductase I) (GenBank accession number EU424321) and TRII (tropinone reductase II) (GenBank(R) accession number EU424322) from the solanaceous plant Anisodus acutangulus by rapid amplification of cDNA ends.

Three new compounds from endophytic fungus L10 of Cephalotaxus hainanensis.

Three new compounds, 4-hydroxyphenethyl-2'-hydroxypropanoate (1), 6-(1',2'-dimethyloxiran-1'-yl)-4-methoxy-3-methyl-2H-pyran-2-one (2), 6-(1-hydroxyethyl)-4-methoxy-3-methyl-2H-pyran-2-one (3), along with the two known compounds, nectriapyrone (4) and wermopyrone (5), have been isolated from the endophytic fungus of Cephalotaxus hainanensis. Their structures were determined on the basis of chemical and spectroscopic methods.

Molecular characterization and expression analysis of two distinct putrescine N-methyltransferases from roots of Anisodus acutangulus.

Putrescine N-methyltransferase (PMT; EC. 2.5.

The relationship between IL-1 gene polymorphism and marginal bone loss around dental implants.

Purpose: Early marginal bone loss around dental implants has been found during the bone healing period before stage II surgery despite a lack of apparent cause, and the etiology of this bone loss is unclear. This study was designed to investigate whether interleukin-1 gene polymorphism is associated with the marginal bone loss around the implants before stage II.

Materials And Methods: One hundred forty-three implants were placed in 59 patients.

[Comparative evaluation of the tiled abutment teeth restored with or without fixed bridge on stress distribution by anisotropic finite element method].

Objective: To compare the tilted abutment teeth restored with or without fixed bridge on stress distribution by anisotropic finite element method when the vertical and the oblique loads are simulated.

Methods: Three-dimensional finite element model was constructed by using SCT image reconstruction technique, self-programming and ANSYS software with the anisotropic elasticity data. The static loads were simulated according to the restored and unrestored situation.

[Analysis of the movement of long axis and the distribution of principal stress in abutment tooth retained by conical telescope].

Objective: To study the movement of long axis and the distribution of principal stress in the abutment teeth in removable partial denture which is retained by use of conical telescope.

Methods: An ideal three dimensional finite element model was constructed by using SCT image reconstruction technique, self-programming and ANSYS software. The static loads were applied.

[Three-dimensional finite element stress distribution and displacement analysis of alveolar ridge retained by conical telescope].

Objective: To study the stress distribution and displacement of edentulous alveolar ridge of removable partial denture which is retained by using conical telescope.

Methods: An ideal three dimensional finite element model was constructed by using SCT image reconstruction technique, self-programming and ANSYS software. The static load was applied.