[Non-membrane mechanisms of antimicrobial peptide P7 against Escherichia coli].

Objective: The molecular mechanism of antimicrobial peptide P7 against Escherichia coli was studied.

Methods: The binding mode between P7 and DNA was analyzed through fluorescence spectroscopy of P7 binding with E. coli genome DNA.

Chinese Women's Experience of Intimate Partner Violence: Exploring Factors Affecting Various Types of IPV.

Using a sample of 553 married and divorced women in a large city in southern China, this study tested the effects of demographic characteristics, risk behaviors, patriarchal ideology, and personal mentality and skills on women's experience of physical violence, psychological violence, controlling behavior, and sexual abuse. Divorced women were more likely than married women to experience all types of IPV. Risk behaviors were consistently related to IPV incidents, whereas the impact of patriarchal ideology and personal mentality and skills was equivocal.

Development of a novel stirrerliquid/solid microextraction method for the separation and enrichment of trace levels of active compounds in traditional Chinese medicine.

A novel stirrer-liquid/solid microextraction method was developed for the separation and enrichment of trace levels of curcumin, bisdemethoxycurcumin, and demethoxycurcumin in Rhizoma Curcumae Longae, Radix Curcumae, and Rhizoma Curcumae before their analysis by high-performance liquid chromatography with ultraviolet detection. In the proposed approach, a magnetic stirrer was immersed in decanol to coat its surface completely with decanol, which was used as an extraction platform. The stirrer coated with decanol is not only a power to agitate the sample solution to constantly update the sample on the stirrer surface but also it can adsorb and extract the target analytes.

The effects of CD147 on the cell proliferation, apoptosis, invasion, and angiogenesis in glioma.

To analyze the effects of extracellular matrix metalloproteinase inducer (CD147) on glioma proliferation, apoptosis, invasion, and angiogenesis. Tissue samples were obtained from 101 glioma cases while normal brain tissues were obtained from 30 brain injury cases. Immunohistochemical assay was performed to detect the expressions of CD147, CD34, and VEGF in tissue samples.

Degradation of triclosan in the presence of p-aminobenzoic acid under simulated sunlight irradiation.

This study aimed to investigate the degradation of triclosan (TCS) in the presence of p-aminobenzoic acid (PABA) under simulated sunlight irradiation (λ ≥ 290 nm). The effect of PABA concentration, pH, dissolved organic matter (DOM), and DOM-hydrolytic Fe(III) species complexes on the photodegradation of TCS in the presence of PABA (TCS) was also studied. The photolysis of TCS obeyed pseudo-first-order kinetics well, and the degradation of TCS enhanced with increasing solution pH (from 3.

Inhibition of cyclodextrins on α-galactosidase.

This work successfully investigated the effects of different influential factors and hydrophobic cavities of cyclodextrins (CDs) on α-galactosidase (α-Gal) by detecting α-Gal activity. The highest inhibitory concentration of three kinds of CDs (α-, β-, and γ-CD) on α-Gal was 10mM. Moreover, the highest inhibition of α-Gal was obtained under the following conditions: reaction time of 90min, temperature of 30°C, and pH 6.

Finite-wavelength surface-tension-driven instabilities in soft solids, including instability in a cylindrical channel through an elastic solid.

We deploy linear stability analysis to find the threshold wavelength (λ) and surface tension (γ) of Rayleigh-Plateau type "peristaltic" instabilities in incompressible neo-Hookean solids in a range of cylindrical geometries with radius R_{0}. First we consider a solid cylinder, and recover the well-known, infinite-wavelength instability for γ≥6μR_{0}, where μ is the solid's shear modulus. Second, we consider a volume-conserving (e.

Identification of Dietetically Absorbed Rapeseed (Brassica campestris L.) Bee Pollen MicroRNAs in Serum of Mice.

MicroRNAs (miRNAs) are a class of small noncoding RNA that, through mediating posttranscriptional gene regulation, play a critical role in nearly all biological processes. Over the last decade it has become apparent that plant miRNAs may serve as a novel functional component of food with therapeutic effects including anti-influenza and antitumor. Rapeseed bee pollen has good properties in enhancing immune function as well as preventing and treating disease.

Self-management program for chronic low back pain: A systematic review and meta-analysis.

Objective: To determine the effectiveness of self-management programs (SMPs) on chronic low back pain (CLBP).

Methods: A search of randomized controlled trials (RCTs) was performed in Pubmed, Cochrane Library, Web of Science, Elsevier, and CINAHL through June, 2015. Two reviewers selected trials, conducted critical appraisal, and extracted data.

Isolation and dynamic expression of four genes involving in shikimic acid pathway in Camellia sinensis 'Baicha 1' during periodic albinism.

Flavonoids are the main flavor components and functional ingredients in tea, and the shikimic acid pathway is considered as one of the most important pathways in flavonoid biosynthesis, but little was known about the function of regulatory genes in the metabolism phenolic compounds in tea plant (Camellia sinensis), especially related genes in shikimic acid pathway. The dynamic changes of catechin (predominant flavonoid) contents were analyzed in this study, and four genes (CsPPT, CsDAHPS, CsSDH and CsCS) involving in shikimic acid pathway in C. sinensis albino cultivar 'Baicha 1' were cloned and characterized.

Cell fusion as the formation mechanism of unreduced gametes in the gynogenetic diploid hybrid fish.

The gynogenetic diploid hybrid clone line (GDH) derived from red crucian carp (♀ RCC) × common carp (♂ CC) possesses the unusual reproductive trait of producing unreduced diploid eggs. To identify the mechanism underlying this phenomenon, we examined the structure, in vivo developmental process and in vitro dynamic development of the GDH gonad. In summary, compared with RCC and CC, GDH showed certain special straits.

[Effect of ptxA and ptxB genes of phosphotransferase system on growth of Streptococcus mutans].

Objective: This study aims to evaluate the effect of ptxA and ptxB genes, which are important genes in the L-ascorbate phosphotransferase system (PTS) of Streptococcus mutans (S. mutans).

Methods: The ptxA-, ptxB-, and ptxAB-double deficient mutant as well as ptxAB-complemented strain were constructed.

Graphene-based terahertz metasurface with tunable spectrum splitting.

We design a tunable terahertz metasurface, which consists of two different trapezoid graphene ribbons patterned in opposite directions on a gold film, separated by a thin dielectric spacer. The two kinds of graphene ribbons can cover a nearly 2π phase shift with high reflection efficiency in different spectral regions so that the metasurface can reflect different frequency waves to totally different directions. By changing the Fermi level of the graphene ribbons, the response frequency of the proposed metasurface can be adjusted, and as a result, tunable spectrum splitting can be realized.

Dispersive Liquid-Liquid Microextraction Combined with High-Performance Liquid Chromatography for the Simultaneous Analysis of Matrine Alkaloids in Traditional Chinese Medicine.

Dispersive liquid-liquid microextraction (DLLME), coupled with high-performance liquid chromatography (HPLC), was developed and introduced for simultaneous extraction and determination of the trace level matrine alkaloids (oxymatrine, matrine, sophoridine and oxysophocarpine) in Composite Kushen Injection and Sophorae Flavescentis Radix. Several factors affecting performance were investigated and optimized, including type and volume of extraction solvent, type and volume of disperser solvent, sample phase pH, shaking time, centrifugation time and rate, and salt concentration in the sample solution. Under optimized conditions, the enrichment factors of DLLME for four matrine alkaloids ranged from 0.

Essential Balm: A Strong Repellent Against Foraging and Defending Red Imported Fire Ants (Hymenoptera: Formicidae).

In the present study, the repellent effects of essential balm, a traditional medicine product in China, was tested against foraging and defending red imported fire ants, Solenopsis invicta Buren, under laboratory and field conditions. The laboratory study showed that both band- (width = 1 cm) and patch-smearing of essential balm at each concentration (0.5, 1, or 2 μl/cm(2)) significantly decreased the number of S.

Magnetic Multivalent Trehalose Glycopolymer Nanoparticles for the Detection of Mycobacteria.

A multivalent trehalose-grafted poly(lactic acid) is synthesized and encapsulated with iron oxide magnetic nanoparticles. The magnetic micelles interact with Mycobacterium smegmatis to form orange clusters. Very little particle interaction is found on Staphylococcus epidermidis 35984 or Escherichia coli ORN 208.

Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines.

Telomerase is a type of reverse transcriptase that is overexpressed in almost all human tumor cells, but not in normal tissues, which provides an opportunity for radiosensitization targeting telomerase. Zidovudine, abacavir and lamivudine are reverse transcriptase inhibitors that have been applied in clinical practice for several years. We sought to explore the radiosensitization effect of these three drugs on human esophageal cancer cell lines.

Distributed estimation and control for mobile sensor networks with coupling delays.

This paper deals with the issue of distributed estimation and control for mobile sensor networks with coupling delays. Based on the Kalman-Consensus filter and the flocking algorithm, all mobile sensors move to a target to increase the quality of gathered data, and achieve consensus on the estimation values of the target in the presence of time-delay and noises. By applying an effective cascading Lyapunov method and matrix theory, stability analysis is carried out.

Notch2 as a promising prognostic biomarker for oesophageal squamous cell carcinoma.

We aimed to examine Notch2 expression in oesophageal squamous cell carcinoma (ESCC) patients and to evaluate its prognostic potential. Immunohistochemical (IHC) staining, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis were utilized to investigate the Notch2 expression status and prognostic value. Furtherly, CCK8 and clonogenic assays were conducted to determine if Notch2 inhibition by shRNA could lead to a decrease in the proliferation and survival of ESCC cells.

Serum miR-1297: a promising diagnostic biomarker in esophageal squamous cell carcinoma.

We aimed to value the diagnostic potential of serum miR-1297 in esophageal squamous cell cancer (ESCC). Its expression level was detected in 156 pairs of patients with ESCC and healthy volunteers using quantitative real-time polymerase chain reaction (qRT-PCR) method. It was statistically decreased in ESCC patients compared with healthy controls.

Association of SOX2 and Nestin DNA amplification and protein expression with clinical features and overall survival in non-small cell lung cancer: A systematic review and meta-analysis.

Up to now, the prognosis of non-small cell lung cancer (NSCLC) is poor. With progress of cancer biology, a number of genes have been investigated for predicting prognosis of NSCLC, such as cancer stem cell markers SRY (sex determining region Y)-box 2 (SOX2) and Nestin. Recently, a series of studies have been performed to examine the associations of SOX2 and Nestin with clinical parameters and prognosis in NSCLC, however, the results were not consistent.

Molecular characterization of a novel ring 6 chromosome using next generation sequencing.

Background: Karyotyping is the gold standard cytogenetic method for detection of ring chromosomes. In this study we report the molecular characterization of a novel ring 6 (r6) chromosome in a six-year-old girl with severe mental retardation, congenital heart disease and craniofacial abnormalities.

Methods: Cytogenetic analysis was performed by conventional karyotyping.

Identification and functional analysis of the L-ascorbate-specific enzyme II complex of the phosphotransferase system in Streptococcus mutans.

Background: Streptococcus mutans is the primary etiological agent of human dental caries. It can metabolize a wide variety of carbohydrates and produce large amounts of organic acids that cause enamel demineralization. Phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays an important role in carbohydrates uptake of S.

Screening and quantification of anticancer compounds in traditional Chinese medicine by hollow fiber cell fishing and hollow fiber liquid/solid-phase microextraction.

Hollow fiber cell fishing, based on HepG-2, SKOV-3, and ACHN cancer cells, and hollow fiber liquid/solid microextraction with HPLC were developed and introduced for researching the anticancer activity of Rhizoma Curcumae Longae, Radix Curcumae, and Rhizoma Curcumae. The structures of curcumin, demethoxycurcumin, and bisdemethoxycurcumin screened were identified and their contents were determined. The compound target fishing factors and cell apoptosis rates under the effect of the three medicines were determined.

Mechanistic Study of Inhibitory Effects of Atorvastatin and Docetaxel in Combination on Prostate Cancer.

Aim: To investigate the effects and mechanisms of docetaxel and atorvastatin administered individually or in combination on prostate cancer cells.

Materials And Methods: Cell growth and apoptosis were determined by the trypan blue exclusion assay and morphological assessment of cells was performed with propidium iodide. NF-κB activity was determined by luciferase reporter gene assay and the western blot assay was used to determine the levels of Bcl-2, phospho-Akt, VEGF, and phospho-Erk1/2.