Polyketides from the mangrove-derived fungus HNNU0006.

Seven polyketides, including an undescribed depsidone () and six previously reported 3,6,8-trihydroxy-1-methylxanthone (), 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (), methyl3-chloro-6-hydroxy-2-(4-hy-droxy-2-methoxy-6-methylphenoxy)-4- methoxybenzoate (), xylarianin A (), 4,5-dihydroxy-6-(6'-methylsalicyloxy)-2-hydro-xymethyl-2-cyclohexen-1-one () and alternariol (), have been isolated from cultures of the mangrove-derived fungus HNNU0006. The structure of compound was elucidated by extensive spectroscopic analysis and X-ray crystallography. Furthermore, all the compounds were evaluated their cytotoxic activities, and compounds and showed weak cytotoxicity against two cell lines Vero and A549 with IC values ranging from 95.

Anti-neuroinflammatory andrastin-type meroterpenoids from the marine-derived fungus HNNU w0032.

A new andrastin-type meroterpenoid penimerodione A (), and three known analogues (-), were isolated from the culture of a marine-derived fungus HNNU w0032 by the guidance of MS/MS-based molecular networking. The planar structure of was established by extensive NMR spectroscopic and HRESIMS analyses, and the absolute configuration was elucidated by a single-crystal X-ray diffraction. Compound showed significant inhibitory effect on NO production in LPS-stimulated BV-2 macrophages with an IC value of 5.

Alkaloid-metabolites from the mangrove-derived fungus Penicillium robsamsonii HNNU0006.

Nine metabolites, including three undescribed alkaloids pyripyropenes VW (1-2), penicioxa A (4), two previously reported pyripyropene A (3), oxaline (5), three grisephenone-type xanthone derivatives (6-8), and a diphenyl ether derivative 4-chloro-7,4'-dihydroxy-5,2'-dimethoxy-2-methylformate-6'-methybenzophone (9), were isolated from cultures of the mangrove-derived fungus Penicillium robsamsonii HNNU0006. Their structures were determined by spectroscopic analysis, ECD calculations, together with DP4+ probability analysis. Furthermore, all the isolated compounds were tested for cytotoxicity and anti-phytopathogenic fungal activities.

Drimane-type sesquiterpenoids and their anti-inflammatory evaluation from HNNU0524.

Chemical investigation of a culture broth from the marine-derived fungus HNNU0524 yielded two new compounds including a drimane-type sesquiterpenoid named pyrrnoxin A () and a benzoic acid derivative, pyrrnoxin B (), together with three related known analogues (-). The chemical structures of and were determined by detailed analysis of spectroscopic data, single-crystal X-ray crystallography, quantum mechanics-based DP4+ and ECD calculations. Compounds and moderately inhibited NO production of lipopolysaccharide-induced microglia cells BV2 with IC values of 26.

Callnudoids A-H: Highly modified labdane diterpenoids with anti-inflammation from the leaves of Callicarpa nudiflora.

Eight undescribed 3,4-seco-norlabdane diterpenoids, callnudoids A-H, as well as two known analogues were isolated from the leaves of Callicarpa nudiflora. The structures were elucidated using spectroscopic methods and were compared with published NMR spectroscopic data. The absolute configurations of callnudoids D and E were defined based on ECD data or single-crystal X-ray diffraction.

New alkaloids from the noni juice with potential α-glucosidase inhibitory activity.

Four new alkaloids, nonialkaloids A-D (1-4) and six known analogues (5-10) were isolated from the noni juice. Among the new compounds, 1 and 2 are indole alkaloids with a seven-membered fused N-heterocyclic ring, 3 and 4 are quaternary ammonium derivatives. The structures were elucidated by extensive NMR and MS analysis, while the absolute configurations of the stereogenic carbons were established based on quantum-chemical electronic circular dichroism calculations or the modified Mosher's method.

Phenolic glycosides from the stems of Homalium stenophyllum.

The phytochemical investigation of the stems of Homalium stenophyllum afforded seven new phenolic glycosides (1-5 and 8-9) and two known compounds (6 and 7). Their structures were elucidated by comprehensive analyses of NMR spectroscopic, mass spectrometric data and chemical hydrolysis. Additionally, their anti-inflammatory activities against the NO production in LPS-induced macrophages were evaluated.

Three new unsaturated fatty acids from marine-derived fungus sp. SCAU150.

Four unsaturated fatty acid derivatives including three new pantheric acids (), together with three known polyketides (), were isolated from a culture broth of the marine-derived fungus sp. SCAU150. Their complete structures were determined by NMR and HRESIMS data analyses.

Two new 2,5-diketopiperazine derivatives from mangrove-derived endophytic fungus S30.

Two new 2,5-diketopiperazines derivatives (), together with eight known analogs (), were isolated from a culture broth of an endophytic fungus S30, derived from mangrove . Their complete structures were determined by a detailed analysis of spectroscopic data and ECD calculations. The antimicrobial activity and neuroprotective activity of these isolated compounds were also evaluated.

Ansamycin derivatives from the marine-derived Streptomyces sp. SCSGAA 0027 and their cytotoxic and antiviral activities.

Fourteen ansamycin derivatives including seven new herbimycins G-L (1-6) and divergolide O (7), and seven known analogues were isolated from a culture broth of the marine-derived Streptomyces sp. SCSGAA 0027. Their complete structures were determined by detailed analysis of spectroscopic data and quantum chemical calculations.

A new coumarin derivative from the stems of the endangered plant .

A preliminary phytochemical investigation of the stems of the endangered plant led to the isolation of a new coumarin derivative (named ulmuselactone A, ) and eight known compounds (-). The new structure was elucidated by detailed analysis of comprehensive spectroscopic methods, and its absolute configuration was established by comparing experimental and calculated electronic circular dichroism (ECD) spectra. The isolated compounds were evaluated for their antibacterial activities.

A new phenol derivative isolated from mangrove-derived fungus sp. HJ002.

A new phenol derivative, 3-chloro-5-hydroxy-4-methoxyphenylacetic acid methyl ester (), along with five known compounds methyl 4-hydroxyphenylacetate (), cytosporone B (), ()-striatisporolide A (), ()-butanedioic acid () and ergosterol () were isolated from the mangrove-derived fungus sp. HJ002. Their structures were established by spectroscopic methods, GIAO based C NMR chemical shift calculations and comparison with the data of literature.

Four New Insecticidal Xanthene Derivatives from the Mangrove-Derived Fungus sp. JY246.

Four new xanthene derivatives, penicixanthenes A-D (-), and one known compound were isolated from a marine mangrove endophytic fungus sp. JY246 that was obtained from the stem of . Their structures were determined by detailed NMR, MS spectroscopic data, modified Mosher's method, and calculated electronic circular dichroism data.

Bioactive Polyketide Derivatives from the Mangrove-Derived Fungus HJ004.

Five new tetralones, daldiniones A-E (-), three new chromones, 7-hydroxy-5-methoxy-2,3-dimethylchromone (), 5-methoxy-2-propylchromone (), and 7-ethyl-8-hydroxy-6-methoxy-2,3-dimethylchromone (), and two new lactones, helicascolides D and E ( and ), together with nine known metabolites (-, -, and -) were isolated from the mangrove-derived fungus HJ004. The structures and absolute configurations of the new compounds were determined by analyzing MS and NMR data and utilizing GIAO based C NMR chemical shift calculations and quantum chemical electronic circular dichroism (ECD) calculations. Compounds , , and showed inhibitory activities against α-glucosidase with IC values of 13, 15, and 16 μM, respectively.

New dibenzodioxocinone and pyran-3,5-dione derivatives from the deep-sea-derived fungus SCSIO z053.

A new isopentylated dibenzodioxocinone, canescenin A (), and a new isopentylated pyran-3,5-dione derivative, canescenin B (), were isolated from an extract of the deep-sea-derived fungus SCSIO z053. Their structures were elucidated by spectroscopic analysis. It was rare to obtain pyran-3,5-dione derivatives from nature.

Hygrocin C from marine-derived Streptomyces sp. SCSGAA 0027 inhibits biofilm formation in Bacillus amyloliquefaciens SCSGAB0082 isolated from South China Sea gorgonian.

Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived Streptomyces sp. SCSGAA 0027 and reported for the first time to be capable of inhibiting the biofilm formation of Staphylococcus aureus and Bacillus amyloliquefaciens SCSGAB0082 with the production of anti-microbial lipopeptides from South China Sea gorgonian Subergorgia suberosa at concentrations of less than minimum inhibitory concentrations.

Pteridic acids C-G spirocyclic polyketides from the marine-derived Streptomyces sp. SCSGAA 0027.

Five new pteridic acids C-G (1-5) were isolated from a culture broth of the marine-derived actinomycete Streptomyces sp. SCSGAA 0027. Their complete structures were elucidated on the basis of NMR data, modified Mosher's method and quantum chemical calculations.

Antifungal and Antiviral Cyclic Peptides from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020.

A new linear peptide simplicilliumtide I (1) and four new cyclic peptides simplicilliumtides J-M (2-5) together with known analogues verlamelins A and B (6 and 7) were isolated from the deep-sea-derived fungal strain Simplicillium obclavatum EIODSF 020. Their structures were elucidated by spectroscopic analysis, and their absolute configurations were further confirmed by chemical structural modification, Marfey's and Mosher's methods. Compounds 2, 6, and 7 showed significant antifungal activity toward Aspergillus versicolor and Curvularia australiensis and also had obvious antiviral activity toward HSV-1 with IC values of 14.

Penicillenols from a deep-sea fungus Aspergillus restrictus inhibit Candida albicans biofilm formation and hyphal growth.

Penicillenols (A1, A2, B1, B2, C1 and C2) were isolated from Aspergillus restrictus DFFSCS006, and could differentially inhibit biofilm formation and eradicate pre-developed biofilms of Candida albicans. Their structure-bioactivity relationships suggested that the saturation of hydrocarbon chain at C-8, R-configuration of C-5 and trans-configuration of the double bond between C-5 and C-6 of pyrrolidine-2,4-dione unit were important for their anti-biofilm activities. Penicillenols A2 and B1 slowed the hyphal growth and suppressed the transcripts of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4.

Screening of Anti-Biofilm Compounds from Marine-Derived Fungi and the Effects of Secalonic Acid D on Biofilm.

Biofilm formation of is one of its mechanisms of drug resistance. Antibiofilm screening of 106 compounds from marine-derived fungi displayed that 12 compounds inhibited biofilm formation by >50% at the concentration of 100 μg/ml, and only secalonic acid D (SAD) and B inhibited by >90% at 6.25 μg/ml without inhibiting cell growth after 24-h incubation.

New Furanone Derivatives and Alkaloids from the Co-Culture of Marine-Derived Fungi Aspergillus sclerotiorum and Penicillium citrinum.

Six new compounds including two furanone derivatives sclerotiorumins A and B (1 and 2), one novel oxadiazin derivative sclerotiorumin C (3), one pyrrole derivative 1-(4-benzyl-1H-pyrrol-3-yl)ethanone (4), and two complexes of neoaspergillic acid aluminiumneohydroxyaspergillin (5) and ferrineohydroxyaspergillin (6) were isolated from the co-culture of marine-derived fungi Aspergillus sclerotiorum and Penicillium citrinum. Compound 3 was the first natural 1,2,4-oxadiazin-6-one. Compound 5 showed significant and selective cytotoxicity against human histiocytic lymphoma U937 cell line (IC  = 4.

Oxalicumone A, a new dihydrothiophene-condensed sulfur chromone induces apoptosis in leukemia cells through endoplasmic reticulum stress pathway.

Oxalicumone A (POA1), a novel dihydrothiophene-condensed sulfur chromone isolated from the marine fungus Penicillium oxalicum SCSGAF 0023, showed cytotoxicity against several cancer cells previously. In this study, its anti-cancer activity and underlying mechanism of this action were investigated in leukemia cells like KG-1a, HL60, U937, and K562. The results showed that POA1 inhibited dose-/time-dependently cell growth and induced apoptosis in leukemia cells.