Objective: To investigate the effects of serum containing Qinbai Qingfei concentrated pellets on expressions of NLRP3 inflammasome in RAW264. 7 cells infected with Mycoplasma pneumoniae( MP) IL-1β.
Methods: RAW264.
The aim of this study was to prepare a liposomal delivery system for rapamycin and study its release characteristics. The results may provide a foundation for the further development of a liposomal delivery system for rapamycin and the establishment of a new active treatment method targeted towards the cellular components of atherosclerotic plaques. The ethanol injection method was used to prepare rapamycin-containing liposomes.
View Article and Find Full Text PDFObjective: To encapsulate the free anthraquiones in Rhizoma et Radix Rhei (rhein, chrysophanol, physcione, emodin and aloeemodin) in liposomes and characterize the liposomes.
Methods: The liposomes were separated from free drug with sephadex G-50 with HPLC determination of the free anthraquinones in liposomes, then its entrapment efficiency was calculated. Ethanol injection method was used to prepare the liposomes with the addition of a suitable amount Ca2+ in the medium.
The aim of this study was to explore the degradation kinetics of water-insoluble lauroyl-indapamide in solutions and predict the stabilities of lauroyl-indapamide encapsulated in liposomes. Buffer-acetone (9:1) was used as the reaction solution and the reaction temperature was maintained at 60 degrees C. The correlation of the apparent degradation constants (k(obs)) of lauroyl-indapamide in liposomes and in buffer-acetone solutions at different pH has been explored.
View Article and Find Full Text PDFA sensitive and simple high-performance liquid chromatographic method with UV detection was developed and validated for the determination of andrographolide in rat whole blood. Carbamazepine was employed as internal standard and the blood sample was extracted with chloroform. Chromatographic separations were achieved on a Chromasil ODS column (250 x 4.
View Article and Find Full Text PDFDivalent or trivalent cations such as Ca2+, Ba2+, Mg2+, Zn2+, Fe2+, Al3+ and Fe3+ can cause a significant increase in the entrapment efficiency of lauroyl-indapamide in liposomes, from about 5% to more than 90%, which suggests that the presence of these ions plays an important role in the encapsulation of lauroyl-indapamide.
View Article and Find Full Text PDFLiposome as a carrier of topotecan (TPT), a promising anticancer drug, has been reported in attempt to improve the stability and antitumor activity of TPT. However, the biodistribution pattern of TPT liposome in vivo and PEG-modified liposome containing TPT have not been studied systemically. In this paper, the in vitro stability and in vivo biodistribution behavior of several liposomes containing TPT with different lipid compositions and PEG-modification were studied.
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