Construction of and evaluation of the immune response to two recombinant pseudorabies viruses expressing the B119L and EP364R proteins of African swine fever virus.

African swine fever (ASF) is an infectious disease caused by ASF virus (ASFV), which is characterized by high infectivity, rapid onset of disease, and a high mortality rate. Outbreaks of ASFV have caused great economic losses to the global pig industry, and there is a need to develop safe and effective vaccines. In this study, two recombinant pseudorabies virus (PRV) strains, rGXGG-2016-ΔgI/ΔgE-EP364R and rGXGG-2016-ΔgI/ΔgE-B119L, expressing the EP364R and B119L protein, respectively, of ASFV, were constructed by homologous recombination technology.

[Effect of Vitamin D3 to Platelet Activation Mediated by Tumor Cell Culture Medium].

Objective: To investigate the effect of vitamin D3 to platelet activation by tumor cell culture medium.

Methods: The peripheral blood platelets of BALB/c mice were isolated. The platelets were activated in 4T1 culture fluid for 24 h.

Percutaneous coronary intervention assisted by invasive mechanical ventilation and intra-aortic balloon pump for acute myocardial infarction with cardiogenic shock: Retrospective cohort study and meta-analyses.

There is little evidence to recommend the optimal invasive mechanical ventilation (IMV) modes and ideal positive end-expiratory pressure stress levels for acute myocardial infarction-cardiogenic shock (AMI-CS) patients. The aim of this study was to compare the mortality outcome in patients with AMI-CS who were treated with percutaneous coronary intervention (PCI) assisted by intra-aortic balloon pump (IABP) + IMV with historical controls. From January 1, 2016 to June 1, 2017, 60 patients were retrospectively enrolled at Tianjin Chest Hospital.

[PM Emission Characteristics and Estimation of Emission Reduction Potential from Typical Industrial Sources in Anyang].

To investigate the characteristics of PM emissions in Anyang, the PM emission inventory from industrial sources of Anyang in 2016 was established based on reasonable estimation method and emission factor. The spatial distribution analysis was carried out using Geographic Information System (GIS) technology. Based on the industry's typical ultra-low emission transformation and coal pressure reduction requirements, three scenarios were set to estimate the PM emission reduction potential of industrial sources from Anyang in 2020.

[Scenario Analysis of PM Concentration Targets and Milestones in China].

Reports of decreasing PM concentrations in some developed countries and regions, as well as the trends of annual average concentrations of PM in the 74 key cities of China from 2013 to 2016 were analyzed. The cities were categorized based on PM concentration ranges and the regions where they are located. The average annual declining rates of PM concentration were calculated for these categories.

[Detection of HHV-8 Virus in 450 Free Blood Donons in Wuhan and Its Genotyping Based on ORFK1 Gene].

Objective: To investigate the infection of HHV-8 virus in free blood donors in Wuhan.

Methods: Whole blood samples were collected from 450 free blood donors in Wuhan, and the genomic DNA extraction and serum collection were performed respectively. And then, the positive rate of HHV-8 was detected by PCR and ELISA, the positive detection rates were compared between them.

A S-Layer Protein of Bacillus anthracis as a Building Block for Functional Protein Arrays by In Vitro Self-Assembly.

S-layer proteins create a cell-surface layer architecture in both bacteria and archaea. Because S-layer proteins self-assemble into a native-like S-layer crystalline structure in vitro, they are attractive building blocks in nanotechnology. Here, the potential use of the S-layer protein EA1 from Bacillus anthracis in constructing a functional nanostructure is investigated, and apply this nanostructure in a proof-of-principle study for serological diagnosis of anthrax.

Detection of Bacillus anthracis spores by super-paramagnetic lateral-flow immunoassays based on "Road Closure".

Detection of Bacillus anthracis in the field, whether as a natural infection or as a biothreat remains challenging. Here we have developed a new lateral-flow immunochromatographic assay (LFIA) for B. anthracis spore detection based on the fact that conjugates of B.

[The impact of ambient particulate matter (PM10) on the population mortality for cerebrovascular diseases-a case-crossover study].

Objective: To analyze the association between the concentration of ambient inhalable particulate matter (PM10) and population mortality for cerebrovascular diseases and to explore the impact of PM10 on cerebrovascular diseases.

Methods: Data including meteorological factors, air pollutants (NO2, SO2 and PM10) and cerebrovascular disease mortality in one district of Beijing from 2004 to 2008 were collected and both symmetric bidirectional case-crossover design and conditional logistic regression model were used to analyze the associations among them.

Results: After adjusting the influence of meteorological factors as daily average temperature and relative humidity, the single pollutant model showed that there was no significant lag effect.

[Assessment on analgesic and anti-inflammatory effects of Tongjingbao optimal formula and analysis on its active components].

Objective: To assess the analgesic and anti-inflammatory effects of Tongjingbao optimal formula and analyze its active components.

Method: Animals were divided into the model group, the Tongjingbao granule group and the Tongjingbao optimal formula group. The mice dysmenorrhea model was induced by oxytocin, and their content of blood calcium and MDA, NO, PGE2 in uterus were determined to assess the analgesic and anti-inflammatory effects of different components in Tongjingbao optimal formula and their impacts.

Rapid detection of Bacillus anthracis spores using a super-paramagnetic lateral-flow immunological detection system.

There is an urgent need for convenient, sensitive, and specific methods to detect the spores of Bacillus anthracis, the causative agent of anthrax, because of the bioterrorism threat posed by this bacterium. In this study, we firstly develop a super-paramagnetic lateral-flow immunological detection system for B. anthracis spores.