[Immunoreactivity and immunogenicity analysis of the recombinant cathepsin L-like protease of Fasciola hepatica in SD rats].

Objective: To analyze the immunoreactivity of recombinant cathepsin L-like proteases (CatL) protein of Fasciola hepatica and its immunogenicity in SD rats.

Methods: The E. coli BL21(DE3) cells harbouring recombinant plasmid pET30a-FhCatL were inoculated in LB medium, and the protein expression was induced with IPTG.

[Immunogenicity analysis of recombinant GST protein of Fasciola hepatica in SD rats].

Objective: To analyze the immunogenicity of recombinant glutathione S-transferase protein of Fasciola hepatica (FhGST) in SD rats.

Methods: The recombinant expression plasmid pET30a-FhGST was transformed into E. coli BL21 (DE3) cells and induced with IPTG for protein expression.

[Cloning, expression and immunogenicity analysis of cathepsin L-like protease of Fasciola hepatica].

Objective: To clone and express the cathepsin L-like protease gene of Fasciola hepatica (FhCL) and investigate the immunogenicity of the recombinant FhCL protein.

Methods: Specific primers were designed according to the reported FhCL gene in GenBank. Using total RNA from adult worms of F.