Identification of Hub genes with prognostic values in colorectal cancer by integrated bioinformatics analysis.

Background: Our study aimed to investigate the Hub genes and their prognostic value in colorectal cancer (CRC) via bioinformatics analysis.

Methods: The data set of colorectal cancer was downloaded from the GEO database (GSE21510, GSE110224 and GSE74602) for differential expression analysis using the GEO2R tool. Hub genes were screened by protein-protein interaction (PPI) comprehensive analysis.

Epithelial changes with corneal punctate epitheliopathy in type 2 diabetes mellitus and their correlation with time to healing.

Background: To study basal epithelial cell (BEC), sub-basal nerve plexus (SBN) and Langerhans cell (LC) density in patients with type 2 diabetes mellitus (T2DM) with corneal punctate epitheliopathy (CPE) and to assess their association with time to healing of CPE.

Methods: Retrospective study of in vivo confocal microscopy (IVCM) in 160 eyes from 160 patients with T2DM diagnosed with CPE due to a single cause. Key exclusion criteria included multiple-causes for CPE or treatment with autologous serum.

Early central and peripheral corneal microstructural changes in type 2 diabetes mellitus patients identified using in vivo confocal microscopy: A case-control study.

The aim of this study was to find early central and peripheral corneal microstructural changes in healthy subjects and type 2 diabetes mellitus (T2DM) patients with/without cornea fluorescein dot staining.This is a prospective case-control study of T2DM patients with/without cornea fluorescein dot staining. Age, sex, duration of diabetes, and serum glycosylated hemoglobin A1c (HbA1c) levels were recorded.

Mitochondrial pathways are involved in Eimeria tenella-induced apoptosis of chick embryo cecal epithelial cells.

Accumulating evidence suggests that Eimeria tenella severely damages the intestinal mucosa in infected poultry, resulting in deadly haemorrhagic typhlocolitis and major economic losses. Damage to host tissue is believed to arise mainly from apoptosis, which is, in general, intimately related to mitochondrial function. However, it is unclear whether mitochondria-dependent apoptotic pathways are specifically involved in parasite-induced apoptosis of chick embryo cecal epithelial cells.

Repeatability and Reproducibility of Noninvasive Keratograph 5M Measurements in Patients with Dry Eye Disease.

Purpose. To determine the intraexaminer repeatability and interexaminer reproducibility of tear meniscus height (TMH) and noninvasive Keratograph tear breakup time (NIKBUT) measurements obtained with the Keratograph 5M (K5M) in a sample of healthy and dry eye populations. Methods.

High-performance planar green light-emitting diodes based on a PEDOT:PSS/CH3NH3PbBr3/ZnO sandwich structure.

Unlabelled: Recently, perovskite-based light-emitting diodes based on organometal halide emitters have attracted much attention because of their excellent properties of high color purity, tunable emission wavelength and a low-temperature processing technique. As is well-known, organic light-emitting diodes have shown powerful capabilities in this field; however, the fabrication of these devices typically relies on high-temperature and high-vacuum processes, which increases the final cost of the product and renders them uneconomical for use in large-area displays. Organic/inorganic hybrid halide perovskites match with these material requirements, as it is possible to prepare such materials with high crystallinity through solution processing at low temperature.

[Role of in vivo confocal microscopy in the management of infectious keratitis].

Infectious keratitis remains a severe ocular condition that can lead to irreversible complications and even blindness. An early diagnosis of an infective microorganism leading to effective treatment may improve the outcome of infectious keratitis.However, the accurate and rapid identification of the infectious agent remains a challenge for the ophthalmologist.

[The research progress of biofilm and biofilm in eye disease].

Bacteria adherent on the active or inactive surface can form biofilms in which they are encased in a hydrated matrix of extracellular polymeric substances, which is so called bacterial biofilm (BBF). BBF has greater virulence and drug resistance, they can also evade the host immune system and lead to immune injury compared with planktonic bacteria. Many common medical infections involving ophthalmic infection are biofilm-associated.

[Study on the clinical manifestation of 438 cases with blepharitis].

Objective: To summarize the clinical characteristics and the therapy effect of blepharitis patients in clinic.

Methods: Retrospective case series study.Four hundred and thirty-eight patients were diagnosed clinically as blepharitis in Beijing Tongren Hospital from Jan 2009 to July 2012.

[Research progresses in treating infectious keratitis with photodynamic therapy].

Photodynamic therapy(PDT) as a new therapeutic method is increasing widely used in ocular surface disease. Along with intensive studies of microorganism inhibition and promoting function in tissue repairing of PDT have been developed, PDT been gradually applied to treating infectious keratitis in clinical. We review mechanism, sterilization and application in infectious keratitis of PDT.

[Analysis of etiology and in vitro drug resistance of ocular fungal infection in period of 2009 - 2010].

Objective: To analyze the distribution and shifting trends of fungal culture specimens and assess the drug sensitivity in vitro.

Methods: Case series study. From January 2009 to December 2010, 1302 specimens were collected.

[Effects of tobramycin dexamethasone eye ointment for blepharitis: multi-center clinical trial].

Objective: To realize the effectiveness and security of Tobramycin and Dexamethasone Eye Ointment for blepharitis treatment. Design Case control studies. Participants 148 patients be diagnosed as blepharitis including 81 cases as research group and 67 cases as control group.

[Features and differentiation of smear cytology in ocular fungal infection].

Objective: To investigate the cytological feature and differentiate diagnosis of ocular fungal infections by smear cytology.

Methods: Experimental study. One thousand two hundred and twenty-six specimens of smear cytology were collected retrospectively during January 2007- December 2010 from Microbial Laboratory of Beijing Institute of Ophthalmology.

[Caution to perform excimer laser corneal ablation on the ametropia with corneal guttata].

It is very important to avoid inadequate patients to perform excimer laser keratectomy in order to prevent from surgical complications. The ametropic eyes with corneal guttata is considered very carefully whether to perform excimer laser corneal ablation or not, especially for the patient with corneal guttata companied by Fuchs endothelial dystrophy family history is contraindicated to perform laser corneal refractive surgery.

[In vivo confocal microscopy study of corneal endotheliitis].

Objective: To study the cellular morphological characteristics and changes of corneal endotheliitis by corneal microstructure in vivo.

Methods: Forty-eight clinical diagnosed patients of corneal endotheliitis were examined by in vivo confocal microscopy. Confocal images of different layers were collected and observed.

[Whether does the granular corneal dystrophy indicate for the excimer laser ablation surgery or not].

With the development of excimer laser technologies, the corneal refractive surgical procedures are improved constantly and the indication of excimer laser keratectomy is expanded. In clinic, PRK or LASEK or PTK or LASIK were performed on the eyes of granular corneal dystrophy with refractive error and the early results were satisfied, but the reoccurrence and server opacity of cornea are worried. Is this corneal disease the indication of excimer laser keratectomy? Nowadays, the exact indication for the disease should be declared in clinic.

[Progresses of in vitro culture and differentiation in conjunctival epithelial stem cells].

Conjunctiva epithelial stem cells are bipotent cells giving rise to both conjunctival epithelial cells and goblet cells. They play a central role in ocular surface homeostasis and wound repair, and also have great capacities of self-renewal, proliferation and differentiation. As a result, conjunctival epithelial stem cells are main resources of seed cells for tissue engineering conjunctiva.

[Morphological characteristics in corneal smear of acanthamoeba keratitis].

Objective: To study the morphological characteristics in corneal smear of acanthamoeba keratitis.

Methods: Experimental study. From May 1991 to December 2007, in Department of Microbiology, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, 159 cases with acanthamoeba keratitis were analyzed for the laboratory results and clinical data.

[Emphasis on prevention and control of trachoma in China].

In China, in order to complete the goal of the elimination of blinding trachoma before 2020, which is presented by the World Health Organization, several steps should be taken as following. First of all, the support at all levels by government is needed, meanwhile, it is very necessary to perform a national epidemiological study about prevalence and risk factors of trachoma and to make the program of the control of trachoma in line with Chinese national condition. Finally, to promote the participation of people with strengthening the propaganda of public health knowledge about trachoma is also important.

[Prevalence of trachoma in the world].

Trachoma is known as a chronic, epidemic and blindness eye disease, which is caused by Chlamydia trachomatis. The infection and transmission of trachoma have great relationship with environment, family and sanitation. Recently, there were reports continuously about the epidemic of trachoma in some countries and districts.

[Operation of cornea grafting micro-surgery robot system].

Objective: To design the robotic mechanism of the cornea grafting micro-surgery system and evaluate its experimental feasibility and efficacy.

Methods: It was an experimental study. Based on computer assisted cornea grafting surgery (CACGS), the prototype about cornea grafting robot, which consists of six subsystems, was developed.

Effect of topical application of terbinafine on fungal keratitis.

Background: Fungal keratitis is a rare but serious corneal disease that may result in loss of vision. The poor prognosis might be due to limited treatment option. This study aimed to evaluate the clinical efficacy of 0.

[Analysis on the susceptibility of fusidic acid for common gram positive bacteria].

Objective: To assess in vitro susceptibility of the fusidic acid, vancomycin, tobramycin and ofloxacin for 8 kinds of ocular gram-positive bacteria.

Methods: It was an experimental study. One hundred strains of ocular gram-positive bacterial isolates selected from Beijing Institute of Ophthalmology, cultivated and identified by the routine, were tested for their susceptibilities to fusidic acid, vancomycin, tobramycin and ofloxacin, with in vitro by the Kirby-Bauer disk diffusion method.

[Etiological analysis of ocular non-viral microbial isolates in children and their antibiotics susceptibility in vitro].

Objective: To analyze the types, distribution characteristics and in vitro susceptibility of antibiotic agents of ocular non-viral microbial isolates in children.

Methods: Experimental research.785 specimens obtained from children below the age of 14 in Beijing Tongren Hospital during 1999 to 2004 were cultured and identified for bacterium, fungus and Acanthamoeba respectively at Beijing Institute of Ophthalmology.