Preoperative clinical and contrasted-enhanced CT features to predict perineural invasion in gallbladder carcinoma: focus on clinical T3-4 stage.

Purpose: To investigate the utility of combining clinical and contrasted-enhanced tomography (CECT) parameters for the preoperative evaluation of perineural invasion (PNI) in gallbladder carcinoma (GBC).

Methods: A total of 134 patients with GBC (male/female, 52/82; age, 64.4 ± 9.

Concurrent Detection of Protein and miRNA at the Single Extracellular Vesicle Level Using a Digital Dual CRISPR-Cas Assay.

The simultaneous detection of proteins and microRNA (miRNA) at the single extracellular vesicle (EV) level shows great promise for precise disease profiling, owing to the heterogeneity and scarcity of tumor-derived EVs. However, a highly reliable method for multiple-target analysis of single EVs remains to be developed. In this study, a igital ual CRISPR-Cas-powered ingle V valuation () system was proposed to enable the concurrent detection of surface protein and inner miRNA of EVs at the single-molecule level.

A Virulence Factor from Targets the Host Chloroplast Proteins to Promote Infection.

Chloroplasts are not only places for photosynthesis, but also participate in plant immunity and are important targets of pathogens. Pathogens secrete chloroplast-targeted proteins (CTPs) that disrupt host immunity and promote infection. (Lib.

An MgVO anode exhibiting enhanced rate capability and stability for lithium storage applications.

The development of anode materials with high rate performance as well as favourable working durability is key for next-generation lithium-ion batteries (LIBs). Here, MgVO is reported as an advanced anode material, using a facile and scalable solution combustion technology. The MgVO anode shows a "near-zero" volume change (<10% over 1000 cycles).

Changes in choroidal thickness in pre-myopic children after repeated low-level red-light therapy and their role in predicting myopia prevention and controlling myopic shift.

Purpose: To investigate longitudinal changes in choroidal thickness (ChT) after 1-year treatment of repeated low-level red-light (RLRL) and their predictive value in efficacy on myopia prevention and myopic shift among pre-myopic children.

Methods: 278 pre-myopic (-0.50 D < spherical equivalent refraction, SER ≤ 0.

HyFormer: a hybrid transformer-CNN architecture for retinal OCT image segmentation.

Optical coherence tomography (OCT) has become the leading imaging technique in diagnosing and treatment planning for retinal diseases. Retinal OCT image segmentation involves extracting lesions and/or tissue structures to aid in the decisions of ophthalmologists, and multi-class segmentation is commonly needed. As the target regions often spread widely inside the retina, and the intensities and locations of different categories can be close, good segmentation networks must possess both global modeling capabilities and the ability to capture fine details.

BFAST: joint dimension reduction and spatial clustering with Bayesian factor analysis for zero-inflated spatial transcriptomics data.

The development of spatially resolved transcriptomics (ST) technologies has made it possible to measure gene expression profiles coupled with cellular spatial context and assist biologists in comprehensively characterizing cellular phenotype heterogeneity and tissue microenvironment. Spatial clustering is vital for biological downstream analysis. However, due to high noise and dropout events, clustering spatial transcriptomics data poses numerous challenges due to the lack of effective algorithms.

A chip-based universal strategy to realize multiplex PCR by using wax films for sealing and controllable release of primers.

Simultaneous detection of multiple nucleic acid targets from a single sample is a common requirement in molecular diagnosis and basic research. Dividing a bulky polymerase chain reaction (PCR) into many isolated small reaction units through microfluidic technology is commonly used to realize this goal. However, previous microfluidic platforms for multiplex PCR suffer from complex structures and strict operation requirements.

Association between dietary patterns and the prognosis of amyotrophic lateral sclerosis in China: a cross-sectional study.

Background: Recently, a growing number of studies have specifically examined the impact of dietary variables on the development and progression of amyotrophic lateral sclerosis (ALS). The purpose of this study was to investigate the correlation between different dietary patterns and Chinese ALS patients' prognosis.

Methods: A retrospective study was conducted by recruiting 590 patients with ALS who attended and were regularly followed at hospitals in Nanjing from 2016 to 2023.

Spatiotemporal modeling of molecular holograms.

Quantifying spatiotemporal dynamics during embryogenesis is crucial for understanding congenital diseases. We developed Spateo (https://github.com/aristoteleo/spateo-release), a 3D spatiotemporal modeling framework, and applied it to a 3D mouse embryogenesis atlas at E9.

Lamination-based organoid spatially resolved transcriptomics technique for primary lung and liver organoid characterization.

Spatial-transcriptomics technologies have demonstrated exceptional performance in characterizing brain and visceral organ tissues, as well as brain and retinal organoids. However, it has not yet been proven whether spatial transcriptomics can effectively characterize primary tissue-derived organoids, as the standardized tissue sectioning or slicing methods are not applicable for such organoids. Herein, we present a technique, lamination-based organoid spatially resolved transcriptomics (LOSRT), for organoid-spatially resolved transcriptomics based on organoid lamination.

In situ forming Hydrogel with adding ZnO Nano-particle for effectively methicillin-resistant Staphylococcus aureus infected frostbite injury.

Unlabelled: Hydrogel has emerged as a promising wound dressing material, and in situ forming hydrogel has emerged as a promising wound dressing recently. But most in situ forming hydrogel are normally unstable. Herein, we report an in-situ forming hydrogel synthesized from poly(Nisopropylacrylamide166---butyl acrylate9)-poly(ethyleneglycol)-poly(N-isopropylacrylamide166---butyl acrylate9) copolymer (P(NIPAM166--nBA9)-PEG-P(NIPAM166-conBA9), denoted as PEP) and zinc oxide nano-particle(ZnO nano-particle) in response to skin temperature.

A spatiotemporal transcriptomic atlas of mouse placentation.

The placenta, a temporary but essential organ for gestational support, undergoes intricate morphological and functional transformations throughout gestation. However, the spatiotemporal patterns of gene expression underlying placentation remain poorly understood. Utilizing Stereo-seq, we constructed a Mouse Placentation Spatiotemporal Transcriptomic Atlas (MPSTA) spanning from embryonic day (E) 7.

Self-Adaptive Magnetic Liquid Metal Microrobots Capable of Crossing Biological Barriers and Wireless Neuromodulation.

Magnetic liquid-bodied microrobots (MRs) possess nearly infinite shape adaptivity. However, they currently confront the risk of structure instability/crushes during shape-morphing in tiny biological environments. This article reports that magnetic liquid metal (LM) MRs (LMMRs) show high structure stability and robust magnetic maneuverability.

Single-cell spatiotemporal analysis of the lungs reveals Slamf9 macrophages involved in viral clearance and inflammation resolution.

How the lung achieves immune homeostasis after a pulmonary infection is not fully understood. Here, we analyzed the spatiotemporal changes in the lungs over a 2-week natural recovery from severe pneumonia in a Syrian hamster model of SARS-CoV-2 infection. We find that SARS-CoV-2 infects multiple cell types and causes massive cell death at the early stage, including alveolar macrophages.

Novel insights into the genetic architecture of pregnancy glycemic traits from 14,744 Chinese maternities.

Glycemic traits are critical indicators of maternal and fetal health during pregnancy. We performed genetic analysis for five glycemic traits in 14,744 Chinese pregnant women. Our genome-wide association study identified 25 locus-trait associations, including established links between gestational diabetes mellitus (GDM) and the genes CDKAL1 and MTNR1B.

Regional/ethnic differences in ocular axial elongation and refractive error progression in myopic and non-myopic children.

Aim: To determine the regional and ethnic differences in ocular axial elongation and refractive error progression in myopic and non-myopic children.

Methods: A retrospective analysis of 15 longitudinal clinical and population-based studies was conducted in the UK, Sweden, Australia (classified as European), China, and Vietnam (classified as East Asian) between 2005 and 2021. A total of 14,593 data points from 6208 participants aged 6-16 years with spherical equivalent from +6 to -6 D were analysed.

An electron-losing regulation strategy for stripping modulation towards a highly reversible Zn anode.

The practical application of aqueous zinc-ion batteries (AZIBs) is hindered by their low coulombic efficiency (CE) and unstable cycle life. Numerous electrolyte-additive-related studies have been performed, but most of the focus has been on the Zn plating process. In fact, practical AZIBs undergo stripping in practice rather than plating in the initial cycle, because the commonly used cathodes in the charged state do not have zinc ions, so a uniform stripping process is crucial for the cell performance of AZIBs.