Improved clinical symptoms and mortality among patients with severe or critical COVID-19 after convalescent plasma transfusion.

There is a Commentary on this article in this issue.

Invasive apocrine carcinoma of the breast: clinicopathologic features and comprehensive genomic profiling of 18 pure triple-negative apocrine carcinomas.

Pure invasive apocrine carcinoma is a rare type of primary breast cancer, constituting ~1% of all breast cancers. Since most pure invasive apocrine carcinomas are triple negative, the lack of targeted therapies for triple-negative breast cancer has fostered efforts to discover actionable molecular targets in these tumors. In this study, we analyzed the clinicopathologic characteristics and comprehensive genomic profiling of 18 patients with pure triple-negative apocrine carcinomas (TNACs) using a 324-gene panel assay (FoundationOne CDx).

MicroRNA-1271-5p inhibits the tumorigenesis of ovarian cancer through targeting E2F5 and negatively regulates the mTOR signaling pathway.

Background: MicroRNA-1271-5p (miR-1271-5p) has been reported to participate in the progression of many human cancers. However, the role of miR-1271-5p still remains unclear in ovarian cancer (OC). Therefore, we explored the effect of miR-1271-5p on the development of OC in present study.

Molecular Subtyping of Triple-Negative Breast Cancers by Immunohistochemistry: Molecular Basis and Clinical Relevance.

Background: Molecular subtyping of triple-negative breast cancers (TNBCs) via gene expression profiling is essential for understanding the molecular essence of this heterogeneous disease and for guiding individualized treatment. We aim to devise a clinically practical method based on immunohistochemistry (IHC) for the molecular subtyping of TNBCs.

Materials And Methods: By analyzing the RNA sequencing data on TNBCs from Fudan University Shanghai Cancer Center (FUSCC) (n = 360) and The Cancer Genome Atlas data set (n = 158), we determined markers that can identify specific molecular subtypes.

Knockdown of Nav1.5 inhibits cell proliferation, migration and invasion via Wnt/β-catenin signaling pathway in oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) is a common type of malignant oral cancer that has a high recurrence rate. Voltage-gated sodium channel Nav1.5 was reported to be highly up-regulated in various types of cancers.

Hydroxypropyl--Cyclodextrin Complexes of Styryllactones Enhance the Anti-Tumor Effect in SW1116 Cell Line.

Styryllactones, a class of compounds obtained from the genus (Annonaceae), have demonstrated antitumor activity. However, the aqueous solubility of these compounds is poor. In this study, we identified the absolute configurations of the previously isolated compounds, which were first isolated in our laboratory, by single-crystal X-ray diffraction analysis using Cu Kα radiation.

Effect of Ultrasonic Surface Impact on the Fatigue Properties of Ti3Zr2Sn3Mo25Nb.

The effect of nano grain surface layer generated by ultrasonic impact on the fatigue behaviors of a titanium alloy Ti3Zr2Sn3Mo25Nb (TLM) was investigated. Three vibration strike-numbers of 24,000 times, 36,000 times and 48,000 times per unit are chosen to treat the surface of TLM specimens. Nanocrystals with an average size of 30 nm are generated.

A rapid and convenient method for on-site detection of MON863 maize through real-time fluorescence recombinase polymerase amplification.

As large-scale planting of genetically modified (GM) crops increases, the development of a rapid and convenient method for on-site detection of GM crops is important. We combined the advantages of recombinase polymerase amplification (RPA) and fluorescence detection to establish a rapid, sensitive, specific, and simple detection platform for on-site detection of MON863 maize. Test samples were added directly to the platform after simple pre-treatment with a DNA extraction-free method.

[Efficacy of Oral Cyclophosphamide for Treatment of Refractory T-cell Large Granular Lymphocytic Leukemia Accompanied with Pure Erythrocyte Aplastic Anemia].

Objective: To explore the clinical effects of oral small dose of cyclophosphamide (CTX) in the treatment of T-cell large granular lymphocytic leukemia (T-LGLL) accompanied with pure red cell aplasia (PRCA).

Methods: The clinical features, characteristics of laboratory examinations and the process of oral small dose of CTX treatment after the ineffective treatment of cyclosporine A combining with prednisone in 1 case of T-LGLL with PRCA were reported and discussed with related references.

Results: The elderly female patient had indolent process, mainly presenting with anemia and significant low hyperplasia of bone marrow erythrocyte cells.

[Provincial prevalence of overweight and obesity among 0-5 years old children in China in 2013].

Objective: To study the provincial prevalence of overweight and obesity among 0-5 years old children in China in 2013.

Methods: Data was from the China Nutrition and Health Surveillance among 0-5 years Children and Lactating Women in 2013. A stratified multistage cluster sampling was used among 0-5 years children from 31 provinces, autonomous regions, and municipalities and selected 55 districts/counties.

A 12-hour comprehensive nutrition care benefits blood glucose level and weight gain and improves outcomes in pregnant women with gestational diabetes mellitus.

Background: Gestational diabetes mellitus (GDM) is associated with adverse outcomes and neonatal complications. Its prevalence has been rapidly increasing over the last decade; it is estimated that one in six pregnant women are diagnosed with GDM. Thus, an effective management approach is necessary for women with GDM.

A highly integrated system with rapid DNA extraction, recombinase polymerase amplification, and lateral flow biosensor for on-site detection of genetically modified crops.

With the large-scale planting of genetically modified (GM) crops, the development of a rapid and convenient method for on-site monitoring GM crops is needed. In this study, a duplex recombinase polymerase amplification (DRPA)-based, quick and simple detection system is presented for on-site detection of GM crops. In this system, a rapid DNA extraction method, a DRPA, and a lateral flow biosensor (LFB) are integrated to allow for rapid DNA extraction and amplification, and fast visualization of the detection results.

Circulating Tumor Cells as a Biomarker to Assist Molecular Diagnosis for Early Stage Non-Small Cell Lung Cancer.

Background And Objective: Compared with tissue biopsy, liquid biopsy is the most preferable non-invasive promising method in personalized medicine, although it has many limitations in isolating circulating tumor cells (CTC). Lung cancer associated mortality is drastically increased due to a shortfall of early-stage detection, which remains a challenge. Herein, we aimed to detect lung cancer at an early-stage using CellCollector device.

Intense basolateral membrane staining indicates HER2 positivity in invasive micropapillary breast carcinoma.

Invasive micropapillary carcinoma is characterized by the inside-out growth of tumor clusters and displays incomplete membrane immunostaining of HER2. According to the 2018 American Society of Clinical Oncology and the College of American Pathologists (ASCO/CAP) HER2-testing recommendation, moderate to intense but incomplete staining could be scored as immunohistochemical 2+. Furthermore, the criteria of immunohistochemical 3+ for this staining pattern are not mentioned.

The role of PDIA3 in myogenesis during muscle regeneration.

Beta 3 (β3) integrin plays an important role in the initiation of myogenesis in adult muscle. Protein disulfide isomerases (PDIs) can activate β3 integrin in various cells to promote cell migration, adhesion and fusion. However, the effect of PDIs on myogenesis during muscle regeneration has not been elucidated.

Discovery and Optimization of Small Molecules Targeting the Protein-Protein Interaction of Heat Shock Protein 90 (Hsp90) and Cell Division Cycle 37 as Orally Active Inhibitors for the Treatment of Colorectal Cancer.

Cell division cycle 37 (Cdc37) is known to work as a kinase-specific cochaperone, which selectively regulates the maturation of kinases through protein-protein interaction (PPI) with Hsp90. Directly disrupting the Hsp90-Cdc37 PPI is emerging as an alternative strategy to develop anticancer agents through a specific inhibition manner of kinase clients of Hsp90. Based on a first specific small-molecule inhibitor targeting Hsp90-Cdc37 PPI (), which was previously reported by our group, we conducted a preliminary investigation of the structure-activity relationships and pharmacodynamic evaluations to improve the potency and drug-like properties.

Effect of lentivirus-mediated CFTR overexpression on oxidative stress injury and inflammatory response in the lung tissue of COPD mouse model.

We aimed to investigate the regulatory mechanism of lentivirus-mediated overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) in oxidative stress injury and inflammatory response in the lung tissue of mouse model of chronic obstructive pulmonary disease (COPD). COPD mouse model induced by cigarette smoke was established and normal mice were used as control. The mice were assigned into a normal group (control), a model group (untreated), an oe-CFTR group (injection of lentivirus overexpressing CFTR), and an oe-NC group (negative control, injection of lentivirus expressing irrelevant sequences).

[Status of hypertension awareness, treatment and control among adults in China in 2010-2012].

Objective: To describe the hypertension awareness, treatment and control among adults in China in 2010-2012.

Methods: Data was from the China Nutrition and Health Surveillance in 2010-2012. A stratified multistage cluster sampling was used from 31 provinces, autonomous regions, and municipalities and selected 150 districts/counties.

[Prevalence of early initiation of breastfeeding among Chinese children aged less than 24 months in 2013].

Objective: To estimate the early initiation of breastfeeding status of Chinese children aged less than 24 months and its influence factors.

Methods: Data of children aged less than 24 months old was collected from Chinese Nutrition and Health Surveillance in 2013. Subjects were selected form 55 survey sites of mainland China by the multilevel stratified equal proportional cluster random sampling and the final effective sample size was 14 825.

[Prevalence and variation of hypotension in Chinese adult residents in 2002-2012].

Objective: To analyze the prevalence and variation of hypotension in Chinese adults residents in 2002 and 2010-2012.

Methods: Data of the blood pressure measurements from 2002 and 2010-2012 China National Nutrition and Health Survey(CNNHS), a multistage stratified cluster random sampling survey was conducted in 2002, and the sample size was 147 488, 64 869 males, 82 619 females, 50 123 urban residents, 97 365 rural residents, 71 078 northern residents and 76 410 southern residents. A multi-stage stratified and population-proportional cluster random sampling method was used in 2010-2012, and the sample size of was 120 427, 52 499 males, 67 928 females, 60 214 urban residents, 60 213 rural residents, 53 863 northern residents and 66 564 southern residents.

Flexible pH sensor based on a conductive PANI membrane for pH monitoring.

pH is a critical parameter used to specify the acidity or alkalinity of an aqueous solution in chemistry, food processing, and medical care. In this study, a conductimetric-type micro pH sensor has been achieved using PANI membrane fabricated on a flexible substrate film aiming to monitor wound healing. The sensor is based on the incorporation of a polyaniline (PANI) membrane, interdigital electrode, and polyimide (PI) substrate.

Targeting Stimulator of Interferon Genes (STING): A Medicinal Chemistry Perspective.

Stimulator of interferon genes (STING) is an adaptor protein that induces the secretion of type I interferons and proinflammatory cytokines and is triggered by cytosolic DNA of pathogen and host origins. Given that STING is a mediator in the immune system, pharmacological modulation of STING has shown viable therapeutic effects for pathogen infection, cancer, and inflammatory diseases. In the past decade, the substantial development in this field has encouraged the discovery of STING modulators.

Heat Shock Protein 90 Inhibitors: An Update on Achievements, Challenges, and Future Directions.

Hsp90 is one of the most important chaperones involved in regulating the maturation of more than 300 client proteins, many of which are closely associated with refractory diseases, including cancer, neurodegenerative diseases, and viral infections. Clinical Hsp90 inhibitors bind to the ATP pocket in the N-terminal domain of Hsp90 and subsequently suppress the ATPase activity of Hsp90. Recently, with the increased understanding of the discrepancies in the isoforms of Hsp90 and the modes of Hsp90-co-chaperone-client complex interactions, some new strategies for Hsp90 inhibition have emerged.