Nucleus pulposus cell-mimicking nanoparticles for cell-specific HIF1A editing to modulate SASP-mediated disc inflammation via autophagy activation.

Traditional methods of plasmid delivery, including viral vectors, lipofection, and electroporation, are widely used for gene editing but have limitations, such as cellular toxicity, limited transfection efficiency in primary cells, and nonspecific side effects. Here, we report the development of nucleus pulposus cell (NPC)-mimicking nanoparticles (HIF1A@NNP) with an NPC membrane as the shell and pcDNA3.1-rHIF1A encapsulated in the core via extrusion.

Subwavelength grating couplers for an ultrathin silicon waveguide.

Subwavelength grating structures are widely used in integrated optical devices due to their compact footprints and flexible refractive index tailoring. In this paper, we present a subwavelength grating (SWG) coupler for a 70-nm-thick silicon waveguide without the upper cladding. The device was designed by using a particle swarm optimization algorithm in 3D finite-difference time-domain simulation.

A novel decision tree algorithm model based on chest CT parameters to predict the risk of recurrence and metastasis in surgically resected stage I synchronous multiple primary lung cancer.

Background: Chest computed tomography (CT) may provide evidence to forecast unexpected recurrence and metastasis following radical surgery for stage I synchronous multiple primary lung cancer (SMPLC).

Objective: This study aims to develop and validate a novel CT-based multi-parametric decision tree algorithm (CT-DTA) model capable of accurate risk assessment.

Design: A multicenter retrospective cohort study.

Lymphatic and Venous Contrast-Enhanced Ultrasound Imaging for Differential Diagnosis of Cervical Lymph Node Metastasis in Thyroid Cancer.

Objective To investigate the value of the novel lymphatic contrast-enhanced ultrasound(LCEUS)and conventional venous contrast-enhanced ultrasound(VCEUS)in the differential diagnosis of benign and malignant cervical lymph nodes in patients with thyroid cancer. Methods Patients with suspected thyroid cancer underwent conventional ultrasound,VCEUS,and LCEUS examinations of cervical lymph nodes before biopsy.The diagnostic abilities of conventional ultrasound,VCEUS,and LCEUS were compared with pathological results as the golden standard.

Wnt5a in keratinocytes contributes to complex regional pain syndrome through the activation of NR2B and MMP9 in rats.

Background: Complex regional pain syndrome (CRPS) is a chronic pain condition characterized by inflammatory features, though the underlying mechanisms remain partly understood. Our study examined whether Wnt5a in skin keratinocytes contributes to CRPS-related pain hypersensitivity by activating downstream N-methyl-D-aspartate receptor subunit 2B (NR2B) and matrix metalloproteinase-9 (MMP9) signaling in rats.

Methods: We developed a cell-culture model to mimic the local inflammation of CRPS and a rat model to mimic the chronic post-ischemia pain experienced by CRPS patients.

Supercooling suppression of microencapsulated capric acid-stearic acid eutectic via lignin-zein stabilized Pickering emulsion for highly efficient thermal energy storage.

This study develops a fully biobased phase change material (PCM) by encapsulating capric acid-stearic acid eutectic (CSE) with a lignin-zein shell. It is driven by core-shell aggregation in Pickering emulsion during solvent exchange. Herein, the introduction of zein effectively promotes core-shell aggregation and stabilizes CSE Pickering emulsion.

One-pot synthesized three-way junction based multiple strand displacement amplification for sensitive assay of H5N1 DNA.

The rapid and sensitive detection of H5N1, a highly pathogenic avian influenza virus, is crucial for controlling its spread and minimizing its impact on public health. In this study, we developed a novel biosensor based on strand displacement amplification (SDA) coupled with CRISPR/Cas12a for highly sensitive detection of H5N1 DNA. The biosensor utilizes a combination of a three-way junction structure, composed of three hairpins (H1, H2, H3), to initiate amplification through SDA, resulting in the production of numerous activators.

Screening for Safe and Efficient Strains with Functions of Lowering Blood Lipids, Blood Glucose, and Blood Pressure.

is a fungus widely used in food fermentation. This study employed microbial technology, combined with microscopic morphological observations and ITS sequence analysis, to isolate, purify, and identify 10 strains of red yeast mold from various products. After the HPLC detection of metabolic products, the M8 strain containing the toxic substance citrinin was excluded.

regulates gut microbiota and increases the effective metabolite luteolin to inhibit MAPK/STAT3 signaling pathway to alleviate allergic rhinitis.

The global prevalence of allergic rhinitis (AR) remains high, posing challenges due to its chronic nature and propensity for recurrence. Gut microbiota dysbiosis contributes to immune dysregulation, impacting AR pathogenesis. () has great potential in regulating immune function to alleviate AR symptoms.

Ultrafine metal nanoparticles isolated on oxide nano-islands as exceptional sintering-resistant catalysts.

Ultrafine nanoparticles (NPs) have attracted extensive research interest, especially in heterogeneous catalysis. However, the inherent sintering propensity of NPs has been a major obstacle to their catalytic stability. Here we report an isolation strategy to preserve highly dispersed ultrafine NPs under extremely harsh conditions.

Roles of Adam8 in Neuroinflammation in experimental ischemic Stroke: Insights from single-cell and ribosome-bound mRNA sequencing.

Stroke remains a leading cause of global mortality, with neuroinflammation significantly exacerbating clinical outcomes. Microglia serve as key mediators of post-stroke neuroinflammation, though the mechanisms driving their migration to injury sites remain poorly understood. In this study, using publicly available single-cell sequencing data (GSE234052), we identified a migration-associated microglial subtype in a murine model of distal middle cerebral artery occlusion (dMCAO).

Modified minimally invasive transforaminal microscope-assisted fusion to treat lumbar foraminal stenosis combined with severe lumbar degenerative diseases.

Purpose: Lumbar foraminal stenosis combined with severe lumbar degenerative disease causing instability, radiculopathy and claudication may require surgery. The surgical treatment goals are decompression, restoration of the height of the disc and foramina and stabilize the spine. We used a modified minimally invasive transforaminal lumbar interbody fusion (TLIF) approach in those patients and evaluated its clinical efficacy.

The myb transcription factor PtoPHL3 positively regulates poplar resistance to canker disease caused by Dothiorella gregaria.

Diseases caused by pathogenic microorganisms impair plant growth, leading to reduced crop yields. While the molecular mechanisms of plant disease response are well understood in annual herbaceous species, they remain largely unknown in perennial woody plants. Here, we found that PtoPHL3, a key transcription factor in poplar's phosphorus starvation response, showed significant expression changes after treatments with salicylic acid (SA) and methyl jasmonate (MeJA), and inoculation of Dothiorella gregaria that causes poplar canker disease.

Hsa_circ_0001756 drives gastric cancer glycolysis by increasing the expression and stability of PGK1 mRNA.

Introduction: Strategies for preventing high glycolysis in tumour cells are urgently needed. CircRNAs (circRNAs) play important roles in glycolysis. However, the mechanism underlying the effects of hsa_circ_0001756 in gastric cancer (GC) remains unclear.

Effect of Enhanced Uterine Involution on Reproductive Performance in Multiparous Ewes.

Rapid uterus involution is essential for minimising interpartum intervals, enhancing reproductive performance, and optimising production in ewes. This study aimed to accelerate the uterine involution of postpartum ewes through a relatively simple hormone treatment approach. In this study, 96 Dorper × Hu F1 ewes were assigned to three groups.

MAZ-mediated N6-methyladenosine modification of ZEB1 promotes hepatocellular carcinoma progression by regulating METTL3.

Background: Hepatocellular carcinoma (HCC) has a hidden onset and high malignancy. Its high metastasis, high recurrence, and short survival time have always been a difficult and hot spot in clinical practice. Our previous study revealed that myc-associated zinc finger protein (MAZ) is highly upregulated in HCC tissues and may promote the proliferation and metastasis of HCC cells by inducing the epithelial-mesenchymal transformation (EMT) process.

Semi-rational engineering of an α-L-fucosidase for regioselective synthesis of fucosyl--acetylglucosamine disaccharides.

α-L-Fucosidases are attractive biocatalysts for the production of bioactive fucosylated oligosaccharides, however, poor regioselectivity and activity for transglycosylation have significantly limited their applications. We have recently derived an α-L-Fucosidase, BF3242, from NCTC9343, which could efficiently synthesize a mixture of Fuc-α-1,3/1,6-GlcNAc, but its 1,3/1,6-regioselectivity was observably affected by reaction temperature. Here, we integrated loop-targeted random mutagenesis and site-directed mutagenesis to engineer the regioselectivity and transglycosylation activity of BF3242.

Electricity Driven Behavior and Self-Cleaning Property of Droplets on Superhydrophobic Anti-pollution Flashover Coating.

The potential of superhydrophobic coatings for anti-pollution flashover should be further explored, focusing on droplet electrical behavior on surfaces. In this study, the electrically driven behavior of droplets on a horizontal superhydrophobic surface was experimentally examined. The characteristics of this behavior were compared and analyzed relative to RTV (room temperature vulcanized silicone rubber).

Diagnostic value of ratio of blood inflammation to coagulation markers in periprosthetic joint infection.

Introduction: Assess the feasibility of utilizing the ratio of blood inflammation to coagulation markers as a potential periprosthetic joint infection (PJI) diagnostic tool.

Materials And Methods: A retrospective analysis was conducted, involving 133 PJI and 93 aseptic loosening patients. Levels of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), platelet count, mean platelet volume, fibrinogen, D-dimer, and ratios of CRP to fibrinogen, ESR to fibrinogen, platelet count and mean platelet volume ratio (PC/MPV), and D-dimer were compared.

Large-scale metabolomic landscape of edible maize reveals convergent changes in metabolite differentiation and facilitates its breeding improvement.

Edible maize is an important food crop, providing energy and nutrients to meet human health and nutritional requirements. However, how environmental pressures and human activity have shaped the edible maize metabolome remains unclear. In this study, we collected 452 diverse edible maize accessions worldwide, comprising waxy, sweet, and field maize.

The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus.

Daptomycin (DAP) is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, and its biosynthesis is precisely regulated by a complex regulatory network. Although the biosynthetic pathway of DAP has been elucidated, the regulatory mechanism governing its biosynthesis at the transcriptional level is not yet fully understood. In the present study, a new transcriptional regulator, DhyR, was identified.

Hsa_circ_0002301 inhibits ferroptosis in gastric cancer by encoding the de novo protein HECTD1-463aa.

Background: CircRNAs are closely related to ferroptosis in gastric cancer cells; however, the mechanism by which circRNAs regulate ferroptosis in gastric carcinogenesis remains unknown. CircRNA-encoded novel peptides are functional products translated from the open reading frames (ORFs) within circular RNAs, demonstrating that circRNAs not only serve as non-coding regulators but also have the capacity to encode biologically active peptides. Compared with noncancerous cells, cancer cells have greater iron requirements, and ferroptosis occurs in response to radiotherapy, chemotherapy, and immunotherapy; therefore, ferroptosis activation may be a potential strategy to overcome the shortcomings of conventional cancer therapy.

Dynamic simulation imaging for complex atmospheric turbulence scenes based on atmospheric coherence length and associated with phase structure function.

Atmospheric turbulence disturbance degrades the quality of long-range image transmission in applications such as marine monitoring and satellite imaging. Simulating an atmospheric turbulence image dataset facilitates the correction of turbulence-distorted images. However, the current simulation algorithms suffer from long computation times, undersampling of frequency, and unrealistic turbulence image simulation.