Target-Navigated CBT-Cys "Stapling" Coupled with CRISPR/Cas12a Amplification for the Photoelectrochemical Nucleic Acid Assay.

Generally, rolling circle amplification (RCA) is based on an enzyme-linked padlock extension reaction. Herein, rapid linking that utilizes click chemistry for joining sticky ends of DNA molecules was developed. The ends of nucleic acid were modified with 2-cyano-6-aminobenzothiazole (CBT) and cystine (Cys-Cys), while glutathione was introduced to break the disulfide bond under target navigation and promote the linkage between CBT and Cys at the terminus of the nucleic acid at pH 7.

CRISPR/Cas12a-based MUSCA-PEC strategy for HSV-1 assay.

In the photoelectrochemical sensing, constant potential excitation to get the photoelectrochemical signal is the main excitation signal mode. Novel method for photoelectrochemical signal obtaining is needed. Inspired by this ideal, a photoelectrochemical strategy for Herpes simplex virus (HSV-1) detection with multiple potential step chronoamperometry (MUSCA) pattern was fabricated using CRISPR/Cas12a cleavage coupled with entropy-driven target recycling.

TiC@WSe as photoelectractive materials coupling with recombinase polymerase amplification for nucleic acid detection.

The photoelectrochemical biosensor based on double redox cycle amplification technology coupled with Tungsten diselenide and MXene-modified electrode was developed. Signal amplification technology is a commonly used method to improve the sensitivity. In this article, in the double redox cycle amplification method, p-aminophenol is used as the signal molecule, and squaric acid acts as a redox indicator.

Self-enhancement photoelectrochemical strategy for kanamycin determination with amino functionalized MOFs.

Based on the self-enhanced photoelectrochemical quality of Cu-MOF-NH, a photoelectrochemical biosensor for kanamycin detection with a Cu-MOF-NH modified electrode was constructed. This biosensor took full advantage of the low electron hole recombination rate due to the ligand-to-metal charge-transfer mechanism of excited electron transfer in Cu-MOF-NH. The kanamycin aptamer was modified onto Cu-MOF-NH by Schiff base reaction.

Chemiluminescence assay for kanamycin based on target recycling strategy.

A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was established based on luminol-functionalized gold nanoparticles as CL nanoprobe materials combined with DNA hairpin structure and carboxyl-modified magnetic beads. Relying on nucleic acid amplification technology, the system can successfully realize the recycling of kanamycin, so that the biosensor can release a large number of luminol-functionalized gold nanoparticles with excellent CL performance even at a low residual levels of kanamycin. The biosensor strategy showed a good linear relationship with kanamycin in the range 0.

Photoelectrochemical Sensing of α-Synuclein Based on a AuNPs/Graphdiyne-Modified Electrode Coupled with a Nanoprobe.

We developed a method for photoelectrochemical (PEC) sensing based on a AuNPs/graphdiyne, as a low background signal composite material, modified electrode coupled with a nanoprobe (probe DNA/DA/MBA/WSe) for sensitive α-synuclein (α-Syn) detection. A tungsten selenide (WSe) nanoflower was first produced with a one-pot solvothermal method and employed as a signal amplification element and the modified substrate of the nanoprobe. The synergy effect between the WSe nanoflower and graphdiyne (GDY) can reduce the photoinduced electron-hole recombination and expedite the spatial charge separation.

Photoelectrochemical aptasensor with low background noise.

In photoelectrochemical (PEC) detection, enhancing the PEC signal and depressing the blank signal are conducive to improve the sensitivity. Because the carbon nanotube (CNT) effectively transfers photogenerated electrons from SnSe to the electrode, the composite nanomaterial CNTs/SnSe generates a strong PEC signal. Methionine (Met), AuNPs, and probe DNA are woven together forming a nanoprobe which is used as a quencher to quench the PEC signal of CNTs/SnSe.

An enzyme linked aptamer photoelectrochemical biosensor for Tau-381 protein using AuNPs/MoSe as sensing material.

Alzheimer's disease is a worldwide health problem and it has attracted extensive attention. Tau protein is an important biomarker in the pathogenesis of Alzheimer's disease. Herein, we devise a in situ enzyme catalysis generating electron donor photoelectrochemical (PEC) biosensor for Tau-381 protein.

Electron Acceptors Co-Regulated Self-Powered Photoelectrochemical Strategy and Its Application for Circulating Tumor Nucleic Acid Detection Coupled with Recombinase Polymerase Amplification.

Biosensor working in a self-powered mode has been widely concerned because it produces a signal when the bias potential is 0 V. However, the self-powered mode is used only when the materials have self-powered properties. Conversion of non-self-powered to self-powered through molecular regulation can solve this problem effectively.

Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent.

Bacterial pathogen control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) was synthesized using the oleylamine reducing method. It was found that Cu/Co/Ni TNA greatly enhanced the chemiluminescence (CL) signal of the hydroxylamine-O-sulfonic acid (HOSA)-luminol system.

One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-HO chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine.

PtCuCo trimetallic alloys (PtCuCo-TAs) are synthesized by one-step reduction. The chemiluminescence (CL) properties of PtCuCo-TAs are studied systemically. PtCuCo-TAs show good catalyzing for luminol-HO system.

Photoelectric current as a highly sensitive readout for potentiometric sensors.

We report here a general strategy to read out potentiometric signals via a photoelectrochemical method. The photocurrent at a working electrode coated with a ZnSe/r-GO composite can be modulated by a polymeric membrane ion-selective electrode that works as a reference electrode.

An assay for Staphylococcus aureus based on a self-catalytic ampicillin-metal (Fe)-organic gels-HO chemiluminescence system with near-zero background noise.

A self-catalytic ampicillin-metal (Fe)-organic gels (AMP-MOGs (Fe))-HO CL system, which is not influenced by transition metal ions, was studied. A method for CL detection of Staphylococcus aureus (S. aureus) based on the AMP-MOGs (Fe)-HO CL system was achieved.

A design for the photoelectrochemical detection of miRNA-221 based on a tungsten diselenide-cysteine-dopamine nanoprobe coupled with mismatched catalytic hairpin assembly target recycling with ultra-low background noise.

A strategy for the photoelectrochemical detection of miRNA with ultra-low background noise was developed using tungsten diselenide-cysteine-dopamine (WSe2/Cys/DA) as a nanoprobe coupled with mismatched catalytic hairpin assembly target recycling. A superior detection limit of 3.3 aM toward miRNA-221 was achieved.

Photoelectrochemical determination of ractopamine based on inner filter effect between gold nanoparticles and graphitic carbon nitride-copper(II) polyphthalocyanine coupled with 3D DNA stabilizer.

Copper(II) polyphthalocyanine (CuPPc) was combined with graphitic carbon nitride (g-CN) to form a heterojunction with enhanced photoelectrochemical (PEC) signal. A sensitive PEC method was developed for determination of ractopamine based on a PEC inner filter effect between gold nanoparticles (AuNPs) and the g-CN/CuPPc. A gold electrode was modified with g-CN/CuPPc and the DNA was linked to the AuNPs.

Photoelectrochemical platform for MicroRNA let-7a detection based on graphdiyne loaded with AuNPs modified electrode coupled with alkaline phosphatase.

In this work, a photoactive material, Graphdiyne (GDY) loaded with AuNPs (AuNPs-GDY), was successfully synthesized. The fabricated material made use of the natural band-gap structure of GDY, which could produce hole-electron pairs and the plasmon resonance effect of AuNPs to obtain a high photoelectrochemical (PEC) response. AuNPs-GDY PEC response changed with the mass ratio of GDY to tetrachloroauric acid.

A photoelectrochemical sensing strategy based on single-layer MoS modified electrode for methionine detection.

MoS, a typical transition metal disulfide, is widely used in the photoelectrochemical (PEC) sensor construction. In general, MoS based PEC sensor are "signal-on" strategies. Surprisingly, we discovered that the PEC response of MoS was quenched by methionine greatly.

Nanogapped Au @ Au-Ag structures coupled with FeO magnetic nanoparticles for the detection of Ochratoxin A.

Contamination of mycotoxins has been a general problem of food safety and afflicts people all around the world. Ochratoxin A (OTA), an abundant and representative food-contaminating mycotoxin, has a detrimental effect on our health. Herein, an ultrasensitive surface-enhanced Raman scattering (SERS) aptasensor was fabricated for OTA detection.

Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin.

In this study, a novel magnetic separation-based multiple systematic evolution of ligands by exponential enrichment (SELEX) was applied to select aptamers simultaneously against three kinds of marine biotoxins, including domoic acid (DA), saxitoxin (STX), and tetrodotoxin (TTX). Magnetic reduced graphene oxide (MRGO) was prepared to adsorb unbound ssDNAs and simplify the separation step. In the multiple SELEX, after the initial twelve rounds of selection against mixed targets and the subsequent four respective rounds of selection against each single target, the three resulting ssDNA pools were cloned, sequenced, and analyzed.

Photoelectrochemical platform for cancer cell glutathione detection based on polyaniline and nanoMoS composites modified gold electrode.

Herein, a visible light photoelectrochemical (PEC) platform based on polyaniline (PANI) and nanoMoS composites as optoelectronic material for glutathione detection without any auxiliary of biomolecules or labeled materials was developed. Firstly, the nanoMoS was prepared via a simple ultrasound exfoliation method. The PANI was synthesized by chemical oxidative polymerization method.

Scaling up an electrochemical signal with a catalytic hairpin assembly coupling nanocatalyst label for DNA detection.

A strategy for the electrochemical detection of DNA based on catalytic hairpin assembly combined with nanocatalyst label-based redox cycling reaction signal amplification. A superior detection limit of 0.3 aM toward DNA can be achieved.

Design of electrochemical detection of thiols based on the cleavage of the disulfide bond coupled with thionine modified gold nanoparticle-assisted amplification.

A new strategy for the electrochemical detection of thiols based on the disulfide cleavage combined with gold nanoparticle (AuNP) assisted signal amplification and an AuNP and graphene (GR) and ionic liquid (IL) modified carbon paste electrode (AuNP/GR/CILE). A superior detection limit of 0.4 pM toward glutathione could be achieved.

Signal amplified strategy based on target-induced strand release coupling cleavage of nicking endonuclease for the ultrasensitive detection of ochratoxin A.

In this work, a new signal amplified strategy based on target-induced strand release coupling cleavage of nicking endonuclease for the ultrasensitive detection of ochratoxin A (OTA) is reported. OTA aptamer (DNA1) and OTA aptamer complementary (DNA2) were immobilized onto a magnetic bead (MB). In the presence of OTA, DNA2 was dissociated and released from the MB.

Indole-3-acetic acid biosensor based on G-rich DNA labeled AuNPs as chemiluminescence probe coupling the DNA signal amplification.

A highly sensitive chemiluminescence (CL) method for detection of phytohormone indole-3-acetic acid (IAA) was developed by using G-rich DNA labeled gold nanoparticles (AuNPs) as CL probe coupling the DNA signal amplification technology. The IAA antibody was immobilized on carboxyl terminated magnetic beads (MBs). In the presence of IAA, antibody labeled AuNPs were captured by antibody functionalized MBs.