Computational exploration of the self-aggregation mechanisms of phenol-soluble modulins β1 and β2 in Staphylococcus aureus biofilms.

The formation of functional bacterial amyloids by phenol-soluble modulins (PSMs) in Staphylococcus aureus is a critical component of biofilm-associated infections, providing robust protective barriers against antimicrobial agents and immune defenses. Clarifying the molecular mechanisms of PSM self-assembly within the biofilm matrix is essential for developing strategies to disrupt biofilm integrity and combat biofilm-related infections. In this study, we analyzed the self-assembly dynamics of PSM-β1 and PSM-β2 by examining their folding and dimerization through long-timescale atomistic discrete molecular dynamics simulations.

NGR-poly(2-ethyl-2-oxazoline)-cholesteryl methyl carbonate enhances the antitumor effect of quercetin liposomes in triple-negative breast cancer.

In this paper, the pH-sensitive targeting functional material NGR-poly(2-ethyl-2-oxazoline)-cholesteryl methyl carbonate (NGR-PEtOz-CHMC, NPC) modified quercetin (QUE) liposomes (NPC-QUE-L) was constructed. The structure of NPC was confirmed by infrared spectroscopy (IR) and nuclear magnetic resonance hydrogen spectrum (H-NMR). Pharmacokinetic results showed that the accumulation of QUE in plasma of the NPC-QUE-L group was 1.

Computational insights into the aggregation mechanism of human calcitonin.

Human calcitonin (hCT) is a peptide hormone that regulates calcium homeostasis, but its abnormal aggregation can disrupt physiological functions and increase the risk of medullary thyroid carcinoma. To elucidate the mechanisms underlying hCT aggregation, we investigated the self-assembly dynamics of hCT segments (hCT, hCT, and hCT) and the folding and dimerization of full-length hCT through microsecond atomistic discrete molecular dynamics (DMD) simulations. Our results revealed that hCT and hCT predominantly existed as isolated monomers with transient small-sized oligomers, indicating weak aggregation tendencies.

A thermosensitive chitin hydrogel with mild photothermal-chemotherapy for facilitating multidrug-resistant bacteria infected wound healing.

Bacterial infection of skin wounds leads to serious health problems, including skin defects, inflammatory pain, and even death. To meet the requirements for successful treatment of complicated wounds, a multifunctional dressing is thus highly desirable. In this work, a thermosensitive hydrogel dressing (HBCA) exhibiting injectability, adaptiveness and mild photothermal antibacterial activity was developed for effective infected wound treatment.

Monitoring Pulmonary Nodule Progression Using Micro-Computed Tomography and Blood Sampling in a Mouse Model.

Micro-computed tomography (micro-CT) is a real-time, intuitive, sensitive, and minimally invasive technique for monitoring changes from pulmonary nodules (PN) to lung cancer (LC). The integration of submandibular vein blood sampling enables rapid, stable, and straightforward detection of imaging and key target alterations during the progression of PN to LC. In this study, we administered a dosage of 100 mg/kg of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in A/J mice to develop a lung adenocarcinoma model.

Self-crystal electret poly(lactic acid) nanofibers for high-flow air purification and AI-assisted respiratory diagnosis.

Particulate matters (PMs), one of the major airborne pollutants, continue to seriously threaten human health and the environment. Here, a self-crystal-induced electret enhancement (SCIEE) strategy was developed to promote the in-situ electret effect and polarization properties of electrospun poly(L-lactic acid) (PLLA) nanofibers. The strategy specifically involved the elaborate pre-structuring of stereocomplex crystals (SCs) with uniform dimensions (∼300 nm), which were introduced into PLLA electrospinning solution as the electrets and physical cross-linking points of high density.

Trends and prevalence of eating disorders in children and adolescents.

Background: Eating disorders (EDs) have increasingly become a public health problem globally, especially among children and adolescents.

Aim: To estimate the burden of EDs in children and adolescents (ages 5-19 years) at the global, regional, and national levels.

Methods: Retrieved from Global Burden of Disease Study 2019 for EDs, including anorexia nervosa and bulimia nervosa, we extracted the disability-adjusted life years (DALYs) and prevalence rates with 95% uncertainty intervals between 1990-2019.

Y01 reduces HFD-induced obesity altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization.

Obesity-related metabolic syndrome is intimately associated with infiltrated adipose tissue macrophages (ATMs), gut microbiota, and metabolic disorders. holds the potential to mitigate obesity; however, there exist strain-specific functionalities and diverse mechanisms, which deserve extensive exploration. This study aims to explore the potential of Y01, isolated from traditional sour whey, in alleviating HFD-induced metabolic syndrome in mice and elucidating its underlying mechanism.

Copper metabolism-related lncRNAs predict prognosis and immune landscape in liver cancer patients.

Background: Characterized by its high mortality and easy recurrence, hepatocellular carcinoma (HCC) poses significant clinical challenges. The association between copper metabolism and development of cancer has been identified. However, the underlying mechanisms of copper metabolism-related long non-coding RNAs (CMRLs) in HCC remain elusive.

Highly electroactive mixed-valence-MoO as an electrochemical sensing for effective detection of p-nitrophenol based on the synergistic effect of valence change and oxygen vacancy.

Transition metal oxides (TMOs) can effectively improve the performance of electrochemical detection due to their unique electronic structure and redox properties. However, the lack of reproducibility and the electrical activity of TMOs prepared from conventional preparation methods limit their further development. In this work, amorphous MoO with reductive Mo(V) was successfully synthesized by one-step electrodeposition, and it has excellent detection performance for p-nitrophenol (PNP).

Prioritizing landscapes for mitigating the impacts of onshore wind farms on multidimensional waterbird diversity in the Yellow Sea.

Ongoing wind energy developments play a key role in mitigating the global effects of climate change and the energy crisis; however, they have complex ecological consequences for many flying animals. The Yellow Sea coast is considered as an ecological bottleneck for migratory waterbirds along the East Asian-Australasian flyway (EAAF), and is also an important wind farm base in China. However, the effects of large-scale onshore wind farms along the EAAF on multidimensional waterbird diversity, and how to mitigate these effects, remain unclear.

High-efficiency respiratory protection and intelligent monitoring by nanopatterning of electroactive poly(lactic acid) nanofibers.

The advent of multifunctional nanofibrous membranes (NFMs) has led to the development of next-generation air filters that are ready to intercept fine particulate matters (PMs) and monitor the respiratory diseases. However, it is still challenging to fabricate biodegradable NFMs featuring the desirable combination of high filtration efficiencies, low air resistance, and intelligent real-time monitoring. Herein, a hierarchical nanopatterning approach was proposed to functionalize the stereocomplexed poly(lactic acid) (PLA) (SC-PLA) nanofibers via the combined electrospinning of SC-PLA and electrospray of CNT@ZIF-8 nanohybrids.

Targeted and non-targeted proteomics to identify the urinary protein biomarkers for Wilson disease.

Background: Wilson disease (WD) is a genetic disorder of copper metabolism. Early diagnosis of WD is inherently challenging due to the absence of typical symptoms. This study aimed to identify urinary protein biomarkers for WD using targeted and nontargeted mass spectrometry-based approaches.

Corrigendum: Long non-coding RNA SNHG1 regulates the Wnt/β-catenin and PI3K/AKT/mTOR signaling pathways EZH2 to affect the proliferation, apoptosis, and autophagy of prostate cancer cell.

[This corrects the article DOI: 10.3389/fonc.2020.

Higher density of CD4+ T cell infiltration predicts severe renal lesions and renal function decline in patients with diabetic nephropathy.

Background: More evidence have shown that the combination of immune and inflammatory mechanism was critical in diabetic nephropathy (DN). However, the relationship between CD4+ T cells and the development of DN is still unclear. Therefore, this study will focus on this issue from the perspective of clinicopathology.

Uncertainty analysis method for diagnosing multi-point defects in urban drainage systems.

Urban drainage system (UDS) plays a key role in city urbanization, where defective pipes can lead to seepage. Previous studies have identified the locations of defects in UDS using inverse optimization models. However, the unique optimal solution neglects uncertainty analysis, which may lead to misdiagnosis.

Global, regional, and national burden of gastrointestinal cancers among adolescents and young adults from 1990 to 2019, and burden prediction to 2040.

Background: Gastrointestinal (GI) cancers have heavily burdened public health. Few studies reported GI cancer burden among adolescents and young adults (AYA). To address this gap, we explored the burden of GI cancer among people aged 15-39.

Epigenetic Regulation of Anthocyanin Biosynthesis in 'Purple Rain'.

'Purple Rain' is characterized by its purple leaves and has ornamental applications. A green mutant line NL, which was mutated by line NZ of 'Purple Rain' during tissue culture, shows green leaves instead of the typical purple color of 'Purple Rain'. This study quantified the leaf color traits of NL and a normal 'Purple Rain' line NZ, and uncovered differentially expressed genes involved in flavonoid biosynthesis pathway genes in NL through RNA-Seq analysis.

Clinical decision-making in bone cancer care management and forecast of ICU needs based on computed tomography.

Objective: This study aimed to evaluate the role of computed tomography (CT) imaging in the diagnosis and management of bone cancer during periods of limited access to histopathological testing. We aimed to determine the correlation between CT severity levels and subsequent patient management and care decisions, adhering to established oncological CT reporting guidelines.

Methodology: A retrospective analysis was conducted on 60 symptomatic patients from January 2021 to January 2024.

The CH-type zinc finger transcription factor ZmDi19-7 regulates plant height and organ size by promoting cell size in maize.

The drought-induced protein 19 (Di19) gene family encodes a Cys2/His2 zinc-finger protein implicated in responses to diverse plant stressors. To date, potential roles of these proteins as transcription factors remain largely elusive in maize. Here, we show that ZmDi19-7 gene exerts pivotal functions in regulation of plant height and organ growth by modulating the cell size in maize.

Exposure to particulate matter (PM) weakens corneal defense by downregulating thrombospondin-1 and tight junction proteins.

Background: Fine particulate matter (PM) induces ocular surface toxicity through pyroptosis, oxidative stress, autophagy, and inflammatory responses. However, the precise molecular pathways through which PM causes corneal damage remain unclear. This study aims to investigate the underlying mechanisms by exposing human corneal epithelial cells (HCECs) to PM.

Highly air-permeable and dust-holding protective membranes by hierarchical structuring of electroactive poly(lactic acid) micro- and nanofibers.

The application of biodegradable electrospun poly(lactic acid) (PLA) fibrous membranes (FMs) toward respiratory protection has long been dwarfed by the poor electret effect and short service life. Herein, a micro-on-nano (MON) approach was proposed to fabricate highly electroactive dual-scale poly(lactic acid) (DS-PLA) FMs consisting of inner-layer nanofibers (667 nm) and outer-layer microfibers (1.22 µm).

Sensitive detection of Hg(II) on MoS/NiS based on interfacial engineering to accelerate the Ni/Ni cycle: Identification the role of atomic-level heterojunction-induced electron transfer in electroanalysis.

The valence change of transition metal ions in nanomaterials can highly enhance the electrochemical detection performance toward heavy metal ions (HMIs), and how to further promote the valence change calls enormous concerns in electroanalysis. In this work, an interfacial engineering that combing the MoS and NiS together to form the MoS/NiS complex is proposed. The density functional theory (DFT) results reveals that the novel atomic-level heterojunction between MoS and NiS will build an internal electric field (IEF), which leads to an enhanced conductivity and valence change behavior of Ni atoms in MoS/NiS complex, resulting in a superior detection performance.