A novel amplification strategy based on target-induced multicomponent nuclease-mediated catalytic hairpin assembly for fluorescent DNA sensor.

Ochratoxin A (OTA) is a highly hazardous mycotoxin widely found in food ingredients and processed products. In response to the demand for food safety, there is an urgent need to establish a highly sensitive, reliable, and cost-effective method for the detection of OTA. In this study, a simple, enzyme-free, sensitive cascade amplification fluorescent strategy was developed to detect OTA based on a magnetic separation system-assisted, multicomponent nuclease (MNAzyme) and its induced catalytic hairpin assembly (CHA).

Editorial for the Bioinformatics of Human Diseases Special Issue.

Bioinformatics plays an ever-increasing role in revealing the complexity of genomic information and how it is related to the susceptibility and pathophysiology of human diseases [...

Oxygen Saturation Sub-analyses Errors in the Dominant Meta-analysis Used to Deimplement Albuterol as a Therapeutic Option for Bronchiolitis.

Objective: Clinical experience and patient-level physiological studies indicate that albuterol transiently reduces oxygen saturation, irrespective of the underlying respiratory condition causing the wheezing. Three revisions of the Cochrane review, Bronchodilators for Bronchiolitis (Review), have found that albuterol temporarily increases oxygen saturation. Rarely, these findings were even statistically significant, but the direction of effect is consistently opposite from physiological studies.

Focused Critical Care Billing Education and Feedback as Part of the Emergency Medicine Residency Curriculum Is Effective.

Background:  Emergency medicine physician compensation models often have a productivity component based on the relative value unit. The primary goal of this study was to determine if implementing critical care billing education through both lectures and individual resident feedback as a formal part of the emergency medicine residency curriculum improved critical care billing among residents.

Methods:  This was a prospective, pre and post, educational intervention, cohort study of emergency medicine resident critical care billing in an academic emergency department.

Evaluation of Integrated Service Strategy Based on Diagnosis of Duct-Dependent Congenital Heart Disease and Neonatal Mortality Data Analysis - Beijing, China, 2021-2022.

Objective: Integrated congenital heart disease (CHD) services were implemented in Beijing in 2022. This study analyzed prenatal diagnosis patterns and neonatal mortality data for duct-dependent CHDs before and after implementation to provide insights for service optimization.

Methods: We conducted a retrospective analysis of 487 cases of duct-dependent CHDs identified through the Beijing Birth Defects Monitoring System from January 2021 to December 2022.

BPIFA1 inhibits periodontitis by regulating the NF-κB/IκB signaling pathway and macrophage M1/M2 polarization.

Background: Periodontitis is a chronic inflammatory disease characterized by tissue destruction and oxidative stress, primarily driven by the imbalance of immune responses. Bactericidal/permeability-increasing fold-containing family A member 1 (BPIFA1) has emerged as a key modulator of inflammation and immune homeostasis.

Objectives: This study investigates the role of BPIFA1 in periodontitis by focusing on its regulatory effects on the NF-κB/IκB signaling pathway and macrophage M1/M2 polarization.

The efficacy of traditional Chinese medicine sequential therapy in non-alcoholic fatty liver disease.

Objective: Sequential therapy in traditional Chinese medicine (TCM) refers to a combination of internal and external treatment methods in a certain order based on syndrome differentiation and therapy. The aim of this study was to evaluate the efficacy of TCM sequential therapy that is given on the basis of conventional management in patients with non-alcoholic fatty liver disease (NAFLD).

Methods: Medical records of one hundred NAFLD patients who received treatment at Zhejiang Provincial Tongde Hospital between February 2023 and April 2024 were retrospectively analyzed.

Construction of an electrochemical sensor based on a conductive Ni(HHTP) nanowires array functionalized with ag nanoparticles@WS QDs for nitrite detection.

A highly sensitive electrochemical nitrite sensor was developed by immobilizing silver nanoparticles (Ag NPs) and tungsten disulfide quantum dots (WS QDs) on a conductive metal-organic frameworks (c-MOFs) consisting of Ni(HHTP) (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene) nanowires array via in-situ growth and photochemical deposition techniques. The electrochemical performance of the AgNPs@WSQDs-Ni(HHTP) sensor was significantly enhanced through the optimization of the growth duration of the Ni(HHTP), the content of WS QDs and the reduction time of Ag NPs. As a result, the prepared sensor exhibited a high sensitivity of 2200.

Prevalence and morphology of middle mesial canals in mandibular first molars and their relationship with anatomical aspects of the mesial root: a CBCT analysis.

Background: This study aims to investigate the prevalence and morphology of middle mesial canal (MMC) in mandibular first molar (M1M) among a Northwestern Chinese population, and to analyze their relationship with anatomical aspects of the mesial root.

Methods: Cone beam computed tomography (CBCT) was utilized to evaluate 898 M1Ms and assess the incidence and morphology of MMC. The following parameters for M1M with or without MMC were obtained: the vertical distance between the first appearance of MMC and canal orifices (D), the distance between mesiobuccal (MB) and mesiolingual (ML) canals (D1), the buccolingual width(L1) and mesiodistal width (L2) of mesial roots, and the flatness degree(L1/L2) of mesial roots.

Prevention strategies of esophageal stenosis after endoscopic resection for superficial esophageal cancer: a Bayesian network meta-analysis.

Introduction: What interventions effectively prevent postoperative stenosis following endoscopic resection (ER) of superficial esophageal cancer? This study aimed to identify effective interventions or combinations through a systematic review and network meta-analysis.

Methods: Six databases were systematically searched for eligible studies up to 30 April 2023, on interventions to prevent esophageal stenosis post-ER. Odds ratios (ORs) evaluated stenosis rate (primary outcome) and complications (secondary outcome), while mean differences (MD) evaluated endoscopic balloon dilatation (EBD) sessions post-stenosis.

Ratiometric fluorescent aptasensor based on DNA-gated FeO@Uio-66-NH and Exo I-assisted signal amplification.

Background: Ochratoxin A (OTA) is toxic secondary metabolites produced by fungi and can pose a serious threat to food safety and human health. Due to the high stability and toxicity, OTA contamination in agricultural products is of great concern. Therefore, the development of a highly sensitive and reliable OTA detection method is crucial to ensure food safety.

First-line cadonilimab plus chemotherapy in HER2-negative advanced gastric or gastroesophageal junction adenocarcinoma: a randomized, double-blind, phase 3 trial.

Programmed cell death protein-1 (PD-1) inhibitors plus chemotherapy have been the standard of care in the first-line treatment of advanced gastric or gastroesophageal junction (G/GEJ) adenocarcinoma; however, the survival benefits are modest in patients with low programmed death ligand 1 (PD-L1) expression. Here we investigated the efficacy and safety of cadonilimab (PD-1/cytotoxic T lymphocyte antigen-4 (CTLA-4) bispecific antibody) plus chemotherapy as first-line treatment in G/GEJ adenocarcinoma. The prespecified interim analysis is reported here.

Exploration of milk flavor: From the perspective of raw milk, pasteurized milk, and UHT milk.

In this study, raw milk was collected from three different grades of pastures and processed by pasteurization, blending and ultra-high temperature sterilization (UHT) in a factory production line with a feed size of 10 tons. Additionally, all samples (from raw milk to UHT milk samples) were analyzed by -nose and GC-MS. Key flavor compounds such as 2-heptanone, hexanal, nonanal, 3-methyl-butanal, and dimethyl sulfide were found.

A chain mediation model reveals the association between depression and cognitive function in the elderly.

The purpose of this study was to explore the relationship between depression, cognitive function, social activities and activities of daily living ( ADL ), and verify whether social activities and ADL have a chain mediating effect between depression and cognitive function. Using the data of the fourth phase of the China Health and Retirement Longitudinal Study ( CHARLS ), 7547 elderly samples were studied. Correlation analysis and Bootstrap method were used to analyze the data to test whether social activities and ADL played a chain mediating role between depression and cognitive function in the elderly.

Effects of different orchard tree pruning residues on the yield and nutrient composition of .

Introduction: As the scale of cultivation expands, challenges such as substrate shortages and rising production costs in mushroom cultivation have become increasingly prominent. Fruit tree pruning residue has the potential to serve as an alternative substrate, offering a sustainable solution. This study evaluates the feasibility of incorporating various types of fruit tree pruning residues into cultivation.

Single-cell transcriptome unveils unique transcriptomic signatures of human organ-specific endothelial cells.

The heterogeneity of endothelial cells (ECs) across human tissues remains incompletely inventoried. We constructed an atlas of > 210,000 ECs derived from 38 regions across 24 human tissues. Our analysis reveals significant differences in transcriptome, phenotype, metabolism and transcriptional regulation among ECs from various tissues.

Current Trends in Chronic Non-Communicable Disease Management: A Bibliometric Analysis of the Past Two Decades.

Background: In recent years, there has been a growing focus on chronic non-communicable diseases (NCD) and their impact on personal and social health. Effective management of NCD is essential for their prevention and treatment. This study aims to utilize bibliometric methods to analyze and summarize the current development and emerging trends in NCD management.

Impact of medical-nursing combined policy pilot on hospitalization frequency of middle-aged and older patients with chronic diseases: a quasi-experimental study based on China Health and Retirement Longitudinal Study.

Background: To address the growing burden of older adult care, the Chinese government has introduced a policy that integrates medical care with elder care, launching two batches of national pilot projects. A majority of the older adult population suffers from one or more chronic diseases, with many experiencing multiple chronic conditions, necessitating support from both elder care and medical services.

Methods: Using panel data from the China Health and Retirement Longitudinal Study (CHARLS) spanning 2011 to 2020, this study employs the difference-in-difference (DID) model to analyze the impact of the integrated medical-nursing policy on the physical health of older patients with chronic diseases.

BPIFA1 alleviates allergic rhinitis by regulating the NF-κB signaling pathway and Treg/Th17 balance.

Aim: Allergic rhinitis (AR) is an allergic condition characterized by inflammation of the nasal mucosa. Bacterial permeability-increasing family member A1 (BPIFA1) exhibits anti-inflammatory properties; however, its impact on AR remains unclear. Aim of this study is to investigate the expression and function of BPIFA1 in AR and its influence on inflammation and immune regulation in a mouse model of AR induced by ovalbumin (OVA).

Adverse events associated with parenteral nutrition support therapy: A pharmacovigilance study.

Background: Parenteral nutrition (PN) plays a crucial role in nutrition support therapy, yet data on related adverse events (AEs) in practical settings are scarce. To address this, we analyzed AE signals associated with PN treatment from the United States Food and Drug Administration Adverse Event Reporting System (FAERS) database.

Methods: We extracted data from the FAERS database, covering the period from the first quarter (Q1) of 2004 to Q1 of 2024.

Incidence and risk factors of ventilator-associated pneumonia in the intensive care unit: a systematic review and meta-analysis.

Background: Ventilator-associated pneumonia (VAP) is a serious complication occurring in critically ill patients receiving mechanical ventilation in the intensive care unit (ICU). This study attempted to analyze VAP incidence in the ICU using a meta-analysis, investigate risk factors for VAP occurrence, and examine influence of VAP on outcomes.

Methods: A search was carried out in the Web of Science, PubMed, Embase, and The Cochrane Library databases to identify studies on incidence and risk factors of VAP in ICU patients.