Anesthetic management of thoracoscopic procedures in neonates: a retrospective analysis of 45 cases.

Background: Advances in medical techniques and equipment have enabled the thoracoscopic repair of certain congenital abnormalities in neonates including congenital esophageal atresia/tracheoesophageal fistula (EA/TEF) and congenital diaphragmatic hernia (CDH). A retrospective analysis was conducted to examine the anesthetic management of neonates (7 days or younger) undergoing thoracoscopic surgery in our hospital department, and to determine the efficacy of anesthetic management in neonates.

Methods: Clinical data from 45 neonates who underwent thoracoscopic surgery in our hospital from December 2015 to March 2020 were retrospectively analyzed.

Lipid degrading microbe consortium driving micro-ecological evolvement of activated sludge for cooking wastewater treatment.

In this study, a lipid degrading microbe consortium (LDMC) was assembled to improve the performance of activated sludge (AS) on cooking wastewater purification. LDMC can rapidly degrade high-level oil (efficiency beyond 93.0% at 5.

Analysis of genetic and clinical factors associated with buprenorphine response.

Background: Buprenorphine, approved for treating opioid use disorder (OUD), is not equally efficacious for all patients. Candidate gene studies have shown limited success in identifying genetic moderators of buprenorphine treatment response.

Methods: We studied 1616 European-ancestry individuals enrolled in the Million Veteran Program, of whom 1609 had an ICD-9/10 code consistent with OUD, a 180-day buprenorphine treatment exposure, and genome-wide genotype data.

Aspirin Attenuates Cardiac Allograft Rejection by Inhibiting the Maturation of Dendritic Cells the NF-κB Signaling Pathway.

Dendritic cells (DCs) serve as an important part of the immune system and play a dual role in immune response. Mature DCs can initiate immune response, while immature or semi-mature DCs induce immune hyporesponsiveness or tolerance. Previous studies have shown that aspirin can effectively inhibit the maturation of DCs.

Effects of environmental factors on soil bacterial community structure and diversity in different contaminated districts of Southwest China mine tailings.

A mass of tailings left by mineral exploitation have caused serious environmental pollution. Although many studies have shown that soil microorganisms have the potential to remediate environmental pollution, the interaction mechanism between microorganisms and the surrounding environment of tailings is still unclear. In this study, 15 samples around pyrite mine tailing were collected to explore the ecological effects of environmental factors on bacterial community.

Lidocaine has antitumor effect on hepatocellular carcinoma via the circ_DYNC1H1/miR-520a-3p/USP14 axis.

Lidocaine can inhibit the malignant development of various human cancers. Circular RNA (circRNA) dynein 1 heavy chain gene (circ_DYNC1H1) acted as a pro-cancer molecule in hepatocellular carcinoma (HCC). This study aimed to explore whether the function of lidocaine was related to the oncogenic circ_DYNC1H1 in HCC.

Effects of ecological environment and host genotype on the phyllosphere bacterial communities of cigar tobacco ( L.).

Microorganisms of plant phyllosphere play an important role in plant health and productivity and are influenced by abiotic and biotic factors. In this study, we investigated the phyllosphere bacterial communities of three cigar tobacco varieties cultivated in Guangcun (GC) and Wuzhishan (WZS), Hainan, China. Metagenomic DNA was extracted from tobacco leaf samples and sequenced by 16S rDNA amplicon sequencing.

Ablation of Survivin in T Cells Attenuates Acute Allograft Rejection after Murine Heterotopic Heart Transplantation by Inducing Apoptosis.

Although studies in oncology have well explored the pharmacological effects of , little is known about its role in allogeneic T-cell responses. Therefore, the present study used a mouse model of acute heart allograft rejection to investigate the protective effect and mechanism of conditional knockout of in T cells. Survivin (encoded by ) was up-regulated in T cells activated and .

Main-Side Chain Hydrogen Bonding-Based Self-Healable Polyurethane with Highly Stretchable, Excellent Mechanical Properties for Self-Healing Acid-Base Resistant Coating.

Developing an autonomous self-healing polyurethane (PU) elastomer with excellent mechanical properties and high ductility has attracted increasing attention. Nowadays, the synthesis of elastomers with excellent mechanical properties and rapid self-healing at room temperature faces a huge challenge. Herein, This work reports a new supramolecular PU with excellent mechanical properties and rapid self-healing at room temperature through the introduction of T-type chain extender into the supramolecular polymer chain.

Lenvatinib enhances T cell immunity and the efficacy of adoptive chimeric antigen receptor-modified T cells by decreasing myeloid-derived suppressor cells in cancer.

Background: Lenvatinib, a tyrosine kinase inhibitor, has been approved for the treatment of several cancers. However, its regulatory activity and related mechanisms on T cell antitumour immunity need to be further investigated.

Methods: The antitumour activity of lenvatinib in immunocompetent and immunodeficient mice was compared to determine the role of T cell immunity.

Impact of pre-pregnancy body mass index, weight gain and blood lipid level during pregnancy on pregnancy outcome in patients with gestational diabetes mellitus.

To explore the effects of pre-pregnancy body mass index (BMI), weight gain and blood lipid level during pregnancy on pregnancy outcome in patients with and without gestational diabetes mellitus(GDM). A total of 12 650 singleton pregnant women without history of hypertension and diabetes who were admitted at Women's Hospital, Zhejiang University School of Medicine for delivery from January 2018 to April 2019 were enrolled in the study. There were 2381 cases complicated with gestational diabetes (GDM group) and 10 269 cases without GDM (non-GDM group).

Selective Gram-Scale C-H Carbenoid Functionalization of -Sulfonylarylamides with a Rhodium Catalyst.

This work describes an effective Cp*Rh-catalyzed C-H carbenoid functionalization of -sulfonylarylamides. Compared to the previous late-stage C-H modification methods of -sulfonylarylamide analogues, this method efficiently achieves the gram-scale transformation with 2.5 mol % Rh-catalyst loading at 0 °C or with a 0.

Coprinus comatus endophytic bacteria characteristics and mechanisms for the cadmium resistance.

Characteristics and resistant mechanisms of macro-fungus endophytic bacteria to cadmium (Cd) have not been well defined. Strains L1 and L3 with Cd-resistant capacity were isolated from the fruiting body of Coprinus comatus, which were identified as Bacillus sp. Under the stress of Cd, the morphologies of both L1 and L3 changed to reduce the threat of Cd.

Characterizing dedifferentiation of thyroid cancer by integrated analysis.

Understanding of dedifferentiation, an indicator of poo prognosis for patients with thyroid cancer, has been hampered by imprecise and incomplete characterization of its heterogeneity and its attributes. Using single-cell RNA sequencing, we explored the landscape of thyroid cancer at single-cell resolution with 46,205 cells and delineated its dedifferentiation process and suppressive immune microenvironment. The developmental trajectory indicated that anaplastic thyroid cancer (ATC) cells were derived from a small subset of papillary thyroid cancer (PTC) cells.

Longitudinal Genomic Evolution of Conventional Papillary Thyroid Cancer With Brain Metastasis.

Background: Brain metastasis is extremely rare but predicts dismal prognosis in papillary thyroid cancer (PTC). Dynamic evaluation of stepwise metastatic lesions was barely conducted to identify the longitudinal genomic evolution of brain metastasis in PTC.

Method: Chronologically resected specimen was analyzed by whole exome sequencing, including four metastatic lymph nodes (lyn 1-4) and brain metastasis lesion (BM).

A Bacillus and Lysinibacillus sp. bio-augmented Festuca arundinacea phytoremediation system for the rapid decontamination of chromium influenced soil.

Phytoremediation as an efficient and eco-friendly soil detoxification method has received widespread attention. In this study, two newly screened Chromium (Cr) reducing strains (Bacillus sp. AK-1 and Lysinibacillus sp.

Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer.

The occurrence and prevalence of colorectal cancer (CRC) is closely associated with age. More than 90% of patients with CRC are diagnosed after 50 years of age. However, CRC incidence of young individuals has been increasing since 1990s, whereas the overall CRC frequency is declining.

Rapid and simple detection of in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay.

Unlabelled: is a devastating pathogen that infects a wide range of plants and causes Phytophthora rot disease, which has resulted in great economic losses in crop production. Therefore, the rapid and practicable detection of is important for disease monitoring and forecasting. In this study, we developed a lateral flow recombinase polymerase amplification (LF-RPA) assay for the sensitive visual detection of .

Improvement of Neoantigen Identification Through Convolution Neural Network.

Accurate prediction of neoantigens and the subsequent elicited protective anti-tumor response are particularly important for the development of cancer vaccine and adoptive T-cell therapy. However, current algorithms for predicting neoantigens are limited by binding affinity data and algorithmic constraints, inevitably resulting in high false positives. In this study, we proposed a deep convolutional neural network named APPM (antigen presentation prediction model) to predict antigen presentation in the context of human leukocyte antigen (HLA) class I alleles.

Multi-omics approaches identify and as candidate autophagic regulators and druggable targets in invasive breast carcinoma.

Autophagy is a critical cellular homeostatic mechanism, and its dysfunction is linked to invasive breast carcinoma (BRCA). Recently, several omics methods have been applied to explore autophagic regulators in BRCA; however, more reliable and robust approaches for identifying crucial regulators and druggable targets remain to be discovered. Thus, we report here the results of multi-omics approaches to identify potential autophagic regulators in BRCA, including gene expression (EXP), DNA methylation (MET) and copy number alterations (CNAs) from The Cancer Genome Atlas (TCGA).

Sizes and ligands tuned gold nanocluster acting as a new type of monoamine oxidase B inhibitor.

Monoamine oxidase inhibitors (MAOIs) are a class of drugs that can be used in the treatment of Parkinson's disease, clinical depression, and anxiety by targeting monoamine oxidase B (MAO). However, the side effects of MAOIs drive the requirement of a new framework of enzyme inhibitors development. In this context, a new type of MAOI has been built on the framework of gold nanoclusters (AuNCs), realizing the transformation from no function of small molecules to MAOI function of ligand-modified AuNCs.

Evaluation of phytoremediation potential of native dominant plants and spatial distribution of heavy metals in abandoned mining area in Southwest China.

A field investigation on the content of heavy metals in soils and dominant plants was conducted in three sites (A<0.5 km, B<1.0 km, C<1.

Design, Synthesis, and Biological Evaluation of Novel Pyrimido[4,5-]indole Derivatives Against Gram-Negative Multidrug-Resistant Pathogens.

Due to the poor permeability across Gram-negative bacterial membranes and the troublesome bacterial efflux mechanism, only a few GyrB/ParE inhibitors with potent activity against Gram-negative pathogens have been reported. Among them, pyrimido[4,5-]indole derivatives represented by GP-1 demonstrated excellent broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria but were limited by hERG inhibition and poor pharmacokinetics profile. To improve their drug-like properties, we designed a series of novel pyrimido[4,5-]indole derivatives based on the tricyclic scaffold of GP-1 and the C-7 moiety of acorafloxacin.

IL-4 inhibits regulatory T cells differentiation by HDAC9-mediated epigenetic regulation.

Regulatory T cells play a crucial role in orchestrating immune response and maintaining immune tolerance, and the expression of the Foxp3 gene is indispensable to the differentiation of regulatory T cells. IL-4 shows strong inhibitory effects on Foxp3 expression and regulatory T cells differentiation, but the detailed mechanisms are still unclear. Here, we revealed that epigenetic modulations are key to this process.