Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation.

Many fish species exhibit remarkable sexual dimorphism, with males possessing numerous advantageous traits for commercial production by aquaculture such as faster growth rate, more efficient food energy utilization for muscle development, and better breeding performance. Several studies have shown that a decrease in the number of primordial germ cells (PGCs) during early development leads predominantly to male progeny. In this study, we developed a method to obtain all-male zebrafish (Danio rerio) by targeted PGC ablation using the nitroreductase/metronidazole (NTR/Mtz) system.

ZNF655 mediated by LINC01210/miR-124-3p axis promotes the progression of gastric cancer.

Gastric cancer (GC) is a common malignant tumor that usually originates from the epithelium of the gastric mucosa. ZNF655 was a suppressor gene of many cancers. However, the mechanism of ZNF655 in GC remains unknown.

Determinants of ecological and carbon footprints to assess the framework of environmental sustainability in BRICS countries: A panel ARDL and causality estimation model.

The purpose of the present study is to evaluate the effects of financial development, human capital, globalization, and renewable energy consumption on ecological and carbon footprint. For this purpose, the data was collected from four countries i.e.

Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.

Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO-NaSeO method, respectively. The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks. The average molecular weights (Mw) of WZP1-3 were 48.

[Influence Factors and Sensitivity of Ozone Formation in Langfang in the Summer].

The diurnal variations in ozone concentrations in the summer were studied using the temperature, wind speed and direction, total cloud cover, and solar radiation intensity data collected in Langfang, China. The ratio of volatile organic compounds to nitrogen oxides (VOCs/NO) and the EKMA curve were studied to analyze the sensitivity of ozone formation. The results showed that:① The ozone generation rate and ozone concentrations were positively correlated with the solar radiation intensity with Pearson correlation coefficients of 0.

Research on rat's pulmonary acute injury induced by lunar soil simulant.

Background: The steps to the moon never stopped after the Apollo Project. Lessons from manned landings on the moon have shown that lunar dust has great influence on the health of astronauts. In this paper, comparative studies between the lunar soil simulant (LSS) and PM2.

The Dynamics and Regulatory Mechanism of Pronuclear H3k9me2 Asymmetry in Mouse Zygotes.

H3K9 methylation is an important histone modification that is correlated with gene transcription repression. The asymmetric H3K9 dimethylation (H3K9me2) pattern between paternal and maternal genomes is generated soon after fertilization. In the present study, we carefully determined the dynamics of H3K9me2 changes in mouse zygotes, and investigated the regulatory mechanisms.

[Fragmentation pathways of five furocoumarine using line ion trap with orbitrapmass spectrometry].

Objective: The multiple levels fragmentations of five furocoumarine (psoralen, xanthotoxin, bergapten, oxypeucedanin, and byakangelicol) in Angelica dahurica have been demonstrated using LTQ-Orbitrap mass spectrometry with high resolution and high mass accuracy to discover the possible,fragmentation regularity.

Method: Duringcollsion-induced dissociation (CID), the MS(n) data of the five compoundswhich were gained in the positive ion mode at 35ev collision energy by direct injection syrings method were analyzed using Xcalibar 2.0 Software to infer the formula of these fragmentations.

Qualitative and Quantitative Analysis of Lignan Constituents in Caulis Trachelospermi by HPLC-QTOF-MS and HPLC-UV.

A high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass (HPLC-QTOF-MS) and ultraviolet spectrometry (HPLC-UV) was established for simultaneous qualitative and quantitative analysis of the major chemical constituents in Caulis Trachelospermi, respectively. The analysis was performed on an Agilent Zorbax Eclipse Plus C18 column (4.6 mm×150 mm, 5 μm) using a binary gradient system of water and methanol, with ultraviolet absorption at 230 nm.

[Research on blockprinted versions of Yokucho collected at the libraries in mainland China].

There are several blockprinted versions of Yokucho written by Yoshimasu Todo, a representative of the Koho School, spread into inland China. By comparison, it is found that all these versions use the printing blocks of "Borotei's collected blocks". The descriptions for all these versions reveal as follows: Anhui Provincial Library: the 5(th) year of Yisi (1785) of Japanese Tenmei reign, a blockprinted edition of Langkha Bookstore, Yoshidashokento from the Borotei blocks; National Library: the 5(th) year of Yisi (1785) of Japanese Teimei reign, a blockprinted edition of Hei'an Bookstore (Royal Bookstore), Siwento Bookstore from the Borotei blocks; Library of China Academy of Chinese Medical Sciences: the 9(th) year of Renshen (1812) of Japanese Bunkha reign, a blockprinted edition of Kyoto Bookstore, Langkha Bookstore, Yoshidashoken Bookstore from the Borotei blocks; Library of Aademia Sinica: (1) the 9(th) year of Renshen (1812) of Japanese Bunkha reign, Khaga-wo Bookstore, Langkha Bookstore from the Borotei blocks; (2) the 5(th) year of Yisi (1785) of Japanese Tenmei reign, a blockprinted edition of Hei'an Bookstore (Royal Bookstore), Siwento Bookstore from the Borotei blocks; Library of Life Sciences, Shanghai Information Center for Life Sciences, Chinese Academy of Sciences: the 9(th) year of Renshen (1812) of Japanese Bunkha reign, a blockprinted edition of Kyoto Bookstore, Langkha Bookstore, Yoshidashoken Bookstore Borotei blocks.

[Textual research on the edition of WU Kun's books].

WU Kun is a famous physician of the Xin'an School. He wrote a 6-volume Yi fang kao (Textual Research on Recipes) in 1584, a 24-volume Wu zhu su wen (Wu's Annotation of Plain Questions) in 1594, a 6-volume Zhen fang liu ji (Six Collections of Acupuncture and Prescription) in 1618 block-printed and funded by CHENG Biao, and a 2-volume Mai yu (Language of Pulse) with writing date unknown. For Yi fang kao, there were 4 editions, including Ming-dynasty block-printed edition, Japanese and Korean block-printed edition, old hand-copied edition and modern printing edition.

TBP dynamics during mouse oocyte meiotic maturation and early embryo development.

To maintain cell lineage, cells develop a mechanism which can transmit the gene activity information to the daughter cells. In mitosis, TBP (TATA-binding protein), a transcription factor which belongs to TFIID was associated with M phase chromosomes and was proved to be a bookmark for cellular memory. Although previous work showed that TBP was dispensable for mouse oocyte maturation and early embryo development, exogenous TBP protein was detected in the nuclear of oocytes and early embryos.

[Textual research of Bencaogangmu Yizhilu].

Bencaogangmu Yizhilu was compiled by DAI Baoyuan according to Bencao Gangmu in the late Qing dynasty. Due to the limited circulation of the book, it was little studied by people in later ages. Errors could be found concerning the book's content, editions and the author's life.

miR-26b promotes granulosa cell apoptosis by targeting ATM during follicular atresia in porcine ovary.

More than 99% of ovarian follicles undergo atresia in mammals, but the mechanism of follicular atresia remains to be elucidated. In this study, we explored microRNA (miRNA) regulation of follicular atresia in porcine ovary. A miRNA expression profile was constructed for healthy, early atretic, and progressively atretic follicles, and the differentially expressed miRNAs were selected and analyzed.

[Research on the interaction between GSTP1 polymorphism and Helicobacter pylori infection in patients with gastric intestinal metaplaisa].

Objective: To detect the distribution of polymorphism of GSTP1 and infection rate of Helicobacter pylori (H. pylori) in different kinds of gastric intestinal metaplasia (IM) and to explore the relationship between entergenic factor-polymorphism of GSTP1 and extogenic factor-H. pylori infection, to determine the risk of subtype of IM.

[Polymorphism of glutathione S-transferase P1 and correlation there of with carcinogenesis risk of gastric intestinal metaplasia].

Objective: To investigate the distribution of polymorphism of glutathione S-transferase P1 (GSTP1) in different kinds gastric intestinal metaplasia (IM), and the carcinogenesis risk of different types of IM.

Methods: Peripheral blood samples were collected from 87 gastric cancer patients, 87 intestinal metaplasia patients, and 87 healthy persons as controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze the distribution of different alleles of GSTP1.

5-AZA-2'-deoxycytidine (5-AZA-CdR) leads to down-regulation of Dnmt1o and gene expression in preimplantation mouse embryos.

5-AZA-2'-deoxycytidine (5-AZA-CdR) is a demethylating, teratogenic agent and a mutagen, which causes defects in the developing mouse and rat after implantation. Our previous data indicated that 5-AZA-CdR (0.2 and 1.

[Effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells].

Objective: To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells.

Methods: After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1 microg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.

[A molecular epidemiological study on the relationship between the polymorphism of GSTP1 and susceptibility to gastric cancer in northern Chinese].

We analyzed the distribution of glutathione-S-transferase P1 gene (GSTP1) polymorphism in a population from northern China and the relationship between the polymorphic BsmAI site in its exon 5 and gastric cancer susceptibility and evaluated the combined effect of GSTP1 polymorphism and H. pylori infection on gastric cancer. Blood samples were taken from 1,612 subjects in areas of high and low incidence of gastric cancer.

[Induction of apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z by curcumin].

Background & Objective: Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern China. Radiotherapy is a major therapy for NPC in early phase,while proper chemotherapy is necessary for NPC in late phase. Some chemotherapeutic drugs have been used to treat tumors by inducing apoptosis of tumor cells.