Molecular characterization of a Fus3/Kss1 type MAPK from Puccinia striiformis f. sp. tritici, PsMAPK1.

Puccinia striiformis f. sp. tritici (Pst) is an obligate biotrophic fungus that causes the destructive wheat stripe rust disease worldwide.

[Cloning and transcriptional profiling of PsNCS1 from Puccinia striiformis f. sp. tritici].

Objective: We cloned PsNCS1 encoding neuronal calcium sensor from Pst and analyzed its transcriptional profile.

Methods: A full-length cDNA of PsNCS1 was cloned by using RT-PCR in combination with cDNA library screening, the sequence was analyzed with different bioinformatic tools and the gene expression pattern was characterized via real-time RT-PCR.

Results: PsNCS1 (Genbank accession no.

[Cloning and transcriptional profiling of a cell division cycle gene PsCdc2 from stripe rust fungus during incompatible and compatible interaction between wheat and Puccinia striiformis f. sp. tritici].

Objective: We cloned a cell division cycle PsCdc2 from Puccinia striiformis f. sp. tritici (Pst) and analyze its expression profile.

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses.

Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp.