[Research progress on biosynthesis of sesquiterpenoids in Atractylodes lancea].

The traditional Chinese medicine Atractlodis Rhizoma is the dried rhizome of the Asteraceae herbal plant Atractylodes lancea, and it has the functions of drying dampness and strengthening the spleen, removing wind and dissipating cold, and brightening the eyes. The sesquiterpenoids in A. lancea are the main ingredients of its pharmacological activities in clinical practice, including atractylone, β-eudesmol, and hinesol, which possess anti-inflammation, antibacterial, antiviral, and hepatoprotective effects.

[Tissue distribution of volatile compounds in secretory cavities of different rhizome sections of wild Atractylodes lancea and A. lancea transplanted for different years].

Laser capture microdissection(LCM) combined with gas chromatography-mass spectrometry(GC-MS) was employed to investigate the distribution of volatile compound in the secretory cavities of different tissues(cortex, phloem, xylem, and pith) in different rhizome sections of wild Atractylodes lancea. The same method was used to study the metabolic characteristics of the volatile compounds in the secretory cavities of different rhizome tissues of A. lancea transplanted for 1-3 years.

Genome resequencing reveals the genetic basis of population evolution, local adaptation, and rewiring of the rhizome metabolome in .

The formation of high-quality Chinese medicinal materials is a micro-evolutionary process of multiple genes involving quantitative inheritance under environmental stress. is a traditionally used medicinal plant in China that is broadly distributed and possesses a considerable amount of essential oils. However, to date, limited research has been conducted to characterize the genetics and metabolites of shaped by natural variation.

[Research progress on biological characteristics and propagation technology of Atractylodes lancea].

Atractylodes lancea is a perennial herb of the Asteraceae family and is one of the well-known traditional Chinese medicine(TCM). Several studies have documented polyene alkyne and sesquiterpenoid compounds as the main bioactive compounds of A. lancea, especially atractylodin, atractylon, β-eudesmol, and hinesol in its rhizomes, which possess anti-virus, anti-inflammation, hypoglycemic, anti-hypoxia, liver protection, and diuresis activities.

Allochthonous Isolates Boost Herb Quality at the Cost of Rhizome Growth.

is a perennial herb whose rhizome (AR) is a valuable traditional Chinese medicine with immense market demand. The cultivation of faces outbreaks of root rot and deterioration in herb quality due to complex causes. Here, we investigated the effects of spp.

Genetic and clinical characterization of familial renal glucosuria.

Background: Familial renal glucosuria (FRG) is a hereditary disorder caused by variants in encoding sodium-glucose cotransporter 2 (SGLT2). In this study, we aimed to characterize proximal tubule solute transport, glucagon secretion and the genotype-phenotype relationship in FRG patients.

Methods: We sequenced and in 21 FRG patients and measured the renal threshold of glucose (RT) in 15 patients.

First Report of Causing Powdery Mildew on in China.

Astragalus mongholicus Bge. [A. membranaceus Bge.

[Research progress in microevolutionary process of excellent traits and quality of Dao-di herbs].

Dao-di herbs are the treasure of Chinese materia medica and one of the characteristic research objects of traditional Chinese medicine(TCM). Probing into the microevolution of Dao-di herbs can help to reveal their biological essence and quality formation mechanisms. The progress in molecular biology and omics provides the possibility to elucidate the phylogenetic and quality forming characteristics of Dao-di herbs at the molecular level.

Impact of intelligent information-based perioperative care on postoperative rehabilitation, complications, and quality of life of patients undergoing gastrointestinal surgery.

To investigate the impact of perioperative intelligent information-based care on postoperative rehabilitation, complications, and quality of life of patients in the operating room. Retrospective analysis of information on 84 patients who underwent gastrointestinal surgery in our hospital from May 2021 to May 2022 were divided into to control group (n = 42) and observation group (n = 42) according to different care modalities. The control group received conventional care, while the observation group received intelligent information-based perioperative care.

Authenticating the geographic origins of rhizome chemotypes in China through metabolite marker identification.

Introduction: is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.

Method: This study aimed to identify the chemotypes of from different areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC-MS/MS.

Induction and metabolomic analysis of hairy roots of Atractylodes lancea.

Atractylodes lancea is an important source of traditional Chinese medicines. Sesquiterpenoids are the key active compounds in A. lancea, and their presence determines the quality of the material.

[DUS testing guidelines for new varieties of Chinese medicinal plants].

A rich diversity of wild medicinal plant resources is distributed in China, but the breeding of new plant varieties of Chinese medicinal plants started late and the breeding level is relatively weak. Chinese medicinal plant resources are the foundation for new varieties breeding, and the plant variety rights(PVP) are of great significance for the protection and development of germplasm resources. However, most Chinese medicinal plants do not have a distinctness, uniformity, and stability(DUS) testing guideline.

Mild shading promotes sesquiterpenoid synthesis and accumulation in Atractylodes lancea by regulating photosynthesis and phytohormones.

Atractylodes lancea rhizome (AR) has high medicinal and economic value. A previous study has reported that the accumulation of sesquiterpenoids in AR has obvious advantages under bamboo canopy. A concrete shade value to promote the cultivation of high-quality AR has not been established.

An automated magnetic bead extraction method for measuring plasma metanephrines and 3-methoxytyramine using liquid chromatography tandem mass spectrometry.

Liquid chromatography tandem mass spectrometry (LC-MS/MS) is used routinely in clinical diagnostics; however, automating the sample pretreatment is challenging. We established and evaluated an automated method based on the magnetic bead extraction principle (MBE) to measure normetanephrine (NMN), metanephrine (MN), and 3-methoxytyramine (3-MT). The target analytes were extracted, purified, and concentrated using different solvents and chemical bond-modified magnetic beads transferred via a magnetic bar.

A robust method for simultaneous measurement of serum 25(OH)D, 1,25(OH) D, and 24,25(OH) D by liquid chromatography-tandem mass spectrometry with efficient separation of 3-epi analogs, 23R,25(OH) D , and 4β,25(OH) D.

Background: This study aimed to establish a robust, simple method to detect 25-hydroxyvitamin D (25(OH)D ), 25-hydroxyvitamin D (25(OH)D ), 1,25-dihydroxyvitamin D (1,25(OH) D ), 1,25-dihydroxyvitamin D (1,25(OH) D ), 24,25-dihydroxyvitamin D (24,25(OH) D ), and 24,25-dihydroxyvitamin D (24,25(OH) D ) simultaneously with efficient separation of 3-epi 25(OH)D , 3-epi 24,25(OH) D , 23R,25(OH) D , and 4β,25-dihydroxyvitamin D (4β,25(OH) D ) by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Method: This method was validated according to procedures established by Clinical and Laboratory Standards Institute (CLSI) and then applied in healthy population to determine the distribution of the vitamin D metabolites by LC-MS/MS.

Results: The total-run CV% of 25(OH)D , 25(OH)D , 24,25(OH) D , 24,25(OH) D , 1,25(OH) D , and 1,25(OH) D were 6.

Evaluation of bone metabolism-associated biomarkers in Tibet, China.

Aim: To measure and evaluate the distribution and possible contributing factors of seven bone metabolism-associated biomarkers in Tibet, a plateau province of China.

Methods: A total of 1615 individuals were recruited from Tibet at three different altitudes. The levels and possible contributing factors of serum calcium, serum phosphorus, ALP, 25OHD, PINP, CTX, and PTH were evaluated.

Diverse Intercropping Patterns Enhance the Productivity and Volatile Oil Yield of (Thunb.) DC.

Commercial cultivation of the medicinal plant is significantly restricted by low survival rates and reduced yields. Intercropping can reasonably coordinate interspecific interactions, effectively utilize environmental resources, and increase survival and yield. We conducted a field experiment from 2014 to 2016 to analyze the advantages and effects of intercropping on survival, growth traits, individual volatile oil content, and total volatile oil content.

Heterophilic antibody interference with TSH measurement on different immunoassay platforms.

Introduction: Interference due to the presence of heterophilic antibodies may lead to falsely low or high analyte concentrations, but falsely elevated values are more common in most immunoassay platforms. We report a case of a 53-y old female patient underwent radical thyroidectomy for thyroid papillary carcinoma and the results of TSH in the Siemens Advia Centaur XP after surgery were not suppressed, ranging from 5.73 and 6.

An evaluation of urine and serum iodine status in the population of Tibet, China: No longer an iodine-deficient region.

Objectives: Iodine is a critical trace element for the synthesis of thyroid-related hormones, and either low or high iodine status can lead to thyroid dysfunction. This study aimed to evaluate the iodine status of the Tibetan population.

Methods: From September 2016 to August 2018, we enrolled 1499 healthy adults from three areas of varying altitudes in Tibet.

Clinical efficacy of acetylcysteine combined with tetrandrine tablets on patients with silicosis and its effect on exercise tolerance and pulmonary function.

Clinical efficacy of acetylcysteine combined with tetrandrine tablets in the treatment of silicosis was investigated to observe the exercise tolerance and pulmonary function of the patients. The clinical data of 248 cases of silicosis patients treated in Branch of Tai'an City Central Hospital were retrospectively analyzed in this study, including 116 cases with conventional therapy (control group), and 132 cases treated with acetylcysteine and tetrandrine tablets on the basis of conventional therapy (observation group). The chest tightness, chest pain, cough and sputum production were evaluated by the St.

Effects of calcium dobesilate (CaD) interference on serum creatinine measurements: a national External Quality Assessment (EQA)-based educational survey of drug-laboratory test interactions.

Objectives: Drug-laboratory test interactions (DLTIs) are one of the major sources of laboratory errors. Calcium dobesilate (CaD) interference on serum creatinine testing is a widespread problem that has long been ignored in China. A national EQA-based survey was launched to investigate the current status of CaD interference on creatinine routine methods used in China and enhance the education of CaD interference in clinical laboratories.