[Highly sensitive determination of three kinds of amanitins in urine and plasma by ultra performance liquid chromatography-triple quadrupole mass spectrometry coupled with immunoaffinity column clean-up].

Cases of toxic mushroom poisoning occur frequently in China every year. In particular, mushrooms containing amanitins can cause acute liver damage, with high mortality rates. The symptoms of acute liver damage are experienced 9-72 h after consumption of the mushrooms.

[Analysis of multi-minerals in seafoods in Zhejiang Province].

Objective: To analyze the contents of Ca, K, Na, Mg, Fe, Zn, Cu and Mn in 33 kinds of marine products, such as fish, shrimp, crab, shellfish and mollusk.

Methods: The national standard GB 5009. 268-2016 Determination of multi elements in foods was used for determination.

[Simultaneous determination of cucurbitacin B, E and I in plasma, urine, and melon and fruit vegetables by ultra performance liquid chromatography-triple quadrupole mass spectrometry using atmospheric pressure chemical ionization].

A method for the determination of cucurbitacin B (CuB), cucurbitacin I (CuI) and cucurbitacin E (CuE) in plasma, urine and melon and fruit vegetables was developed by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). The target analytes in plasma and urine were extracted and cleaned-up by solid supported liquid-liquid extraction, while those in melon and fruit vegetables were extracted with acetonitrile and then diluted with water. CuB, CuI and CuE were separated on an XBridge BEH C column (100 mm×3.

[Simultaneous rapid determination of 84 toxic plant constituents in plasma and urine by ultra-performance liquid chromatography- triple quadrupole/linear ion trap mass spectrometry].

An ultra-performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometry (UPLC-Qtrap MS) method was developed for the determination of 84 toxic plant constiuents in plasma and urine. Plasma was precipitated by acetonitrile to remove proteins and then passed through a Prime HLB SPE column to remove phospholipids, while urine was diluted with methanol. Chromatographic separation of the analytes was achieved on an Acquity BEH C column (100 mm×2.

[Determination of coriatin and corianin in plasma and urine using ultra-performance liquid chromatography-triple quadrupole mass spectrometry].

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) method has been developed for the determination of coriatin and corianin in plasma and urine, which are the biomarkers of poisoning caused by Coriaria sinica Maxim. Plasma and urine samples were extracted and purified using a solid supported liquid/liquid extraction method. Chromatographic separation was performed on a Cortecs C column (100 mm×2.

[Determination of monofluoroacetic acid in plasma and urine by stable isotope dilution ion chromatography-triple quadrupole mass spectrometry].

A method was developed for the determination of monofluoroacetic acid (MFA) in plasma and urine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS). A plasma sample was extracted with 3% (v/v) perchloric acid aqueous solution, and the extract was centrifuged to remove the protein and lipids. A urine sample was acidulated with 3% (v/v) perchloric acid aqueous solution.

[Simultaneous rapid determination of 12 microcystins and one nodularin in water by direct injection-ultra performance liquid chromatography-triple quadrupole mass spectrometry].

A rapid method was developed for the simultaneous determination of 12 microcystins (MCs) and one nodularin (NOD) in water by direct injection-ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). The water samples were first diluted with equal volume of methanol, and then filtered through polyether sulfone (PES) syringe filter. The filtrates were directly injected into the UPLC system.

[Fast determination of pericarpium papaveris illegally added in foods by TurboFlow online purification-ultra performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometry].

A fast confirmation method was developed for the determination of the six markers of pericarpium papaveris, morphine, codeine, narcotine, papavarine, thebaine and protopine in foods, by TurboFlow online purification-ultra performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometry (TF-UPLC-QTRAP MS). The sample was extracted with 0.10 mol/L HCl.

[Rapid determination of benzo[]pyrene in foods by ultra performance liquid chromatography-triple quadrupole mass spectrometry with atmospheric pressure chemical ionization].

A method for the determination of benzo[]pyrene in foods was developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based on isotope dilution and molecularly imprinted solid-phase extraction (MIP-SPE). The target analyte in samples was extracted with -hexane after spiked with benzo[]pyrene-d, and purified using MIP-SPE to eliminate most of the coextracts. The separation of benzo[]pyrene was carried out on an XBridge BEH C column with gradient elution of methanol and water.

[Determination of alpha-solanine, alpha-chaconine and solanidine in plasma and urine by ultra-performance liquid chromatography-triple quadrupole mass spectrometry].

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for the determination of alpha-solanine, alpha-chaconine and solanidine in plasma and urine. The sample was acidified with aqueous solution containing 2% (v/v if not specified) formic acid, and then cleaned-up by solid-phase extraction with a mixed-mode cation exchange (MCX) cartridge. The analysis of the glycoalkaloids was carried out on an Acquity UPLC BEH C18 column (50 mm x 2.

[Rapid simultaneous determination of 53 beta-lactam antibiotics and their metabolites in milk by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry].

A rapid method for the determination of 53 beta-lactams and their metabolites residues in milk was developed by ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). The method was based on protein precipitation by adding equal quantity of acetonitrile, followed by the ultra filtration of the extracts. The analysis of the residues was achieved on an ACQUITY BEH C18 column with gradient elution using mobile phases of 0.

[Determination of tetrodotoxin in seafood using graphitized carbon black clean-up with hilic ultra performance liquid chromatography-triple quadrupole mass spectrometry].

Objective: To develop a rapid hilic ultra performance liquid chromatography (UPLC)-mass spectrum (MS)/MS method for determination of tetrodotoxin in seafood.

Methods: The sample of muscle and liver of puffer fish and nassarius were extracted with aqueous solution containing 0.2% (V/V) acetic acid (the extract of liver must be purified through HLB cartridge), and then cleanup of extract was accomplished by solid-phase extraction with a graphitized carbon black cartridge.

[Isolation and identification of shellfish toxins from contaminated blue mussel (Mytilus edulis) from the East China Sea].

Objective: To investigate the contamination of shellfish poisoning of mussels, the poisonous constituents in that were isolated and identified.

Methods: The mussel tissue homogenate was extracted by acetone, and then the acetone extract was partitioned between diethyl ether and water. The ether extract was fractionated by column chromatography over silica gel and further isolated by semi-preparative RP-HPLC, monitored by ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry.

[Rapid simultaneous determination of 42 psychoactive drugs and their metabolites in human plasma and urine by ultra performance liquid chromatography-tandem mass spectrometry].

A rapid method for the simultaneous determination of 42 psychoactive drugs and their metabolites (barbitals, benzodiazepines, tricyclic antidepressants, phenothiazines, etc. ) in human plasma and urine was developed using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After a simple protein precipitation step, the analysis of the drugs and the metabolites was achieved on an Acquity UPLC BEH C18 column by using the gradient elution with ammonium acetate and methanol-acetonitrile (1: 1, v/v) as the mobile phases, and detected by electrospray ionization-tandem mass spectrometry in the multiple reaction monitoring (MRM) mode operated simultaneously in both positive and negative modes by rapid switching, and quantified by matrix-match standard solution.