Berry texture QTL and candidate gene analysis in grape ( L.).

Berry texture is a noteworthy economic trait for grape; however, the genetic bases and the complex gene expression and regulatory mechanism for the diverse changes in berry texture are still poorly understood. In this study, the results suggest that it is difficult to obtain high-mesocarp firmness (MesF) and high-pericarp puncture hardness (PPH) grape cultivars with high pericarp brittleness (PerB). The high-density linkage map was constructed using whole-genome resequencing based on 151 F individuals originating from intraspecific hybridization between the firm-flesh cultivar 'Red Globe' and soft-flesh cultivar 'Muscat Hamburg'.

Autotoxin affects the rhizosphere microbial community structure by influencing the secretory characteristics of grapevine roots.

Autotoxins secreted by roots into the soil can trigger rhizosphere microecological imbalances and affect root secretory properties resulting in conditions such as replanting disease. However, information on the effect of autotoxins on root secretion characteristics and regulation of the composition of rhizosphere microorganisms by altered root exudates is limited. In this study, autotoxin ρ-hydroxybenzoic acid (4-HBA) was added to the soil of potted grapevine seedlings, CO pulse-labeling, and DNA stable isotope probing were used to track the rhizosphere microbiome that assimilates root exudates.

Gene is Associated With Sex Determination in .

In the past decade, progress has been made in sex determination mechanism in . However, genes responsible for sexual differentiation and its mechanism in remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 () in .

Responses of soil bacterial and fungal communities to the long-term monoculture of grapevine.

Soil microorganisms are essential for the long-term sustainability of agricultural ecosystems. However, continuous grapevine replanting can disrupt the stability of soil microbial communities. We investigated the bacterial and fungal abundance, diversity, and community composition in rhizosphere soils with continuous grapevine replanting for 5, 6, 7 (Y5, Y6, and Y7; short-term), and 20 (Y20; long-term) years with high-throughput sequencing.

High-Density Genetic Linkage Map Construction and White Rot Resistance Quantitative Trait Loci Mapping for Genus Based on Restriction Site-Associated DNA Sequencing.

Grape white rot () is a major fungal disease affecting grape yield and quality. Quantitative trait locus (QTL) analysis is an important method for studying important horticultural traits of grapevine. This study was conducted to construct a high-density map and conduct QTL mapping for grapevine white rot resistance.

Bioabsorbable high-purity magnesium interbody cage: degradation, interbody fusion, and biocompatibility from a goat cervical spine model.

Background: Bioabsorbable Mg-based implants have been a focus of orthopedic researches due to their intrinsic advantages in orthopedics surgeries. This study aimed to investigate the performance of bioabsorbable high-purity magnesium (HP Mg, 99.98 wt.

Transcriptome analysis of table grapes (Vitis vinifera L.) identified a gene network module associated with berry firmness.

Berry firmness is one of the main selection criteria for table grape breeding. However, the underlying genetic determinants and mechanisms involved in gene expression during berry development are still poorly understood. In this study, eighteen libraries sampled from Vitis vinifera L.

High-density genetic linkage map construction and cane cold hardiness QTL mapping for Vitis based on restriction site-associated DNA sequencing.

Background: Cold hardiness is an important agronomic trait and can significantly affect grape production and quality. Until now, there are no reports focusing on cold hardiness quantitative trait loci (QTL) mapping. In this study, grapevine interspecific hybridisation was carried out with the maternal parent 'Cabernet sauvignon' and paternal parent 'Zuoyouhong'.

Candidate genes for grape white rot resistance based on SMRT and Illumina sequencing.

Background: White rot is one of the most dangerous fungal diseases and can considerably affect grape berry production and quality. However, few studies have focused on this disease, and thus, finding candidate white rot resistance genes is of great importance for breeding resistant grapevine cultivars. Based on field observations and indoor experiments, the cultivars "Victoria" and "Zhuosexiang" showed significant differences in white rot resistance.

Genetic Diversity in Genes of f. sp. Suggests Horizontal Gene Transfer.

Fusaric acid (FA) is an important secondary metabolite of many Fusarium species and involved in the wilt symptoms caused in banana by f. sp. .

A Cerato-Platanin Family Protein FocCP1 Is Essential for the Penetration and Virulence of f. sp. Tropical Race 4.

f. sp. tropical race 4 ( TR4) is well-known as the causal agent of wilt of banana and is one of the most destructive phytopathogens for banana plants.

[Treatment of coronal shear fracture of the distal end of the humerus by the olecranon osteotomy approach].

Objective: To explore the effectiveness of olecranon osteotomy approach for the treatment of coronal shear fracture of the distal end of the humerus.

Methods: A retrospective analysis was made on the clinical data of 34 patients with the coronal shear fracture of the distal end of the humerus treated by the olecranon osteotomy approach from January 2005 to January 2013. Of 34 cases, there were 15 boys and 19 girls, aged from 17 years to 84 years (mean, 54.

Construction of a high-density genetic map for grape using specific length amplified fragment (SLAF) sequencing.

Genetic maps are important tools in plant genomics and breeding. We report a large-scale discovery of single nucleotide polymorphisms (SNPs) using the specific length amplified fragment sequencing (SLAF-seq) technique for the construction of high-density genetic maps for two elite wine grape cultivars, 'Chardonnay' and 'Beibinghong', and their 130 F1 plants. A total of 372.

[Comparison of clinical effect on locking plate for proximal humeral fracture with or without application of inferomedial screws].

Objective: To compare clinical outcomes of locking plate for proximal humeral fracture whether application of inferomedial screws.

Methods: From January 2012 to July 2013, 46 patients with proximal humeral fracture underwent locking plates were retrospectively analyzed. There were 25 males and 21 females aged from 29 to 80 years old with an average of 55.

SSR and SRAP marker-based linkage map of L.

An F1 population was created by the cross '87-1' × '9-22'. The female parent '87-1' was an extremely early maturing cultivar with strong flavour. The male parent was an excellent breeding line producing large berries maturing late.

An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon.

A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.

[Allelopathy of grape root aqueous extracts].

Taking the tissue-cultured seedlings of grape cultivar Red Globe as test objects, this paper examined the effects of their root aqueous extracts on seedling's growth, with the allelochemicals identified by LC-MS. The results showed that 0.02 g x ml(-1) (air-dried root mass in aqueous extracts volume; the same below), 0.

[Effects of grape-replanting on soil bacterial and fungal populations].

Rhizosphere and non-rhizosphere soil samples were collected from the vineyards having been planted for 3 and 30 years, and PCR-DGGE technique was adopted to study the effects of grape-replanting on the population structure and diversity of soil bacteria and fungi. The bacterial and fungal diversities were higher in 30-year-planted vineyard than in 3-year-planted vineyard, and higher in rhizosphere soil than in non-rhizosphere soil. After 30 years replanting, the population structure of bacteria and fungi approached the same in rhizosphere soil and non-rhizosphere soil but differed from that in fallow soil; while in the 3-year-planted vineyard, the population structure in rhizosphere soil was different from that in non-rhizosphere soil and fallow soil.

[Effects of waterlogging on root respiration intensity and respiratory enzyme activities of sweet cherry].

Taking Meizao/Dongbeishanyingtao (Prunus serrulata G. Don) and Meizao/Mahaleb (P. mahaleb L.

[The changes of three endogenous hormones during flower bud differentiation of Schisandga chinensis].

Objective: To reveal the relation between endogenous hormones and the flower bud differentiation in Schisandga chinensis.

Method: Top buds of extremely short branch and axillary buds of long branch in the same plant of S. chinensis were used as material and the contents of endogenous hormones were measured during different periods of the flower bud differentiation with HPLC.