Metagenomic Next-Generation Sequencing for Accurate Diagnosis of Pneumonia: A Comparative Study with Traditional Methods.

Background: Metagenomic next-generation sequencing (mNGS) is a high-throughput sequencing technique that identifies a wide array of pathogens directly from clinical specimens. This study evaluates the diagnostic value of mNGS in pneumonia (PJP) and compares its efficacy with traditional detection methods, including Grocott's Methenamine Silver (GMS) staining, serum (1-3)-β-D-Glucan (BDG) testing, and Lactate Dehydrogenase (LDH) testing.

Methods: Seventy-eight patients hospitalized between January 2022 and March 2023 with suspected pulmonary infections were included.

Comparison between MRI-negative and positive results and the predictors for a poor prognosis in patients with idiopathic acute transverse myelitis.

Background: Idiopathic acute transverse myelitis (IATM) is a focal inflammatory disorder of the spinal cord that results in motor, sensory, and autonomic dysfunction. However, the comparative analysis of MRI-negative and MRI-positive in IATM patients were rarely reported.

Objectives: The purpose of this study was to compare MRI-negative with MRI-positive groups in IATM patients, analyze the predictors for a poor prognosis, thus explore the relationship between MRI-negative and prognosis.

Practical recommendations for antihypertensive therapy during the primary aldosteronism screening test.

Background: The aldosterone-to-renin activity ratio (ARR) is recommended as a screening test for primary aldosteronism (PA). The use of antihypertensive medication is one of the most important factors to take into account when interpreting the ARR.

Objectives: The goal of this study was to compare the effect of various antihypertensive drug classes on biochemical testing and provide practical recommendations for antihypertensive medication regimens in patients suspected of having PA.

A syndrome featuring developmental disorder of the nervous system induced by a novel mutation in the gene, rarely concurrent immune disorders: a case report.

The expression of TCF20 is the most widespread in brain tissue. TCF20 depletion or mutation can affect the proliferation and differentiation of embryonic neurons, leading to developmental disorder of the central nervous system and subsequent rare syndrome featuring. Here, we report a 3-year-old boy carrying a novel frameshift mutation in c.

Accuracy of implant placement using CAD-CAM tooth-supported surgical guides for an auricular prosthesis in vitro.

Purpose: To evaluate the accuracy of tooth-supported surgical guides used to place implants in auricular prostheses. The accuracy (trueness and precision) of the implant positions was evaluated, and the difference between the surgical guide with and without retention of the external auditory canal (EAC) was compared.

Materials And Methods: This study simulated implant placement in vitro for the treatment of right auricle malformation.

[EGF/PI3K signaling pathway regulates the expression of osteopontin in liver cancer HepG2 cells].

Objective: To investigate the effect of epidermal growth factor (EGF) on the expression of osteopontin (OPN) in liver cancer HepG2 cells.

Methods: Human liver caner cells of HepG2 line were cultured in medium without bovine serum. EGF was added.