Gel-to-Coacervate Transition in Peptide/HA Complexes for MMP-9-Activated Penetration into Tumor Spheroids.

Short phase-separating peptides serve as liquid-based vehicles due to their remarkable fluidity and cell permeability, holding great promise in diffusion-limited applications such as intracellular drug delivery or penetration into deep-seated tumors. However, tuning the phase stability and the phase-transition sensitivity of these coacervates in response to specific pathological signals remains a significant challenge. To tackle this challenge, this study presents a phase-separating peptide/hyaluronic acid (HA) complex coacervate system, which undergoes a solid-to-coacervate transition upon exposure to matrix metalloproteinase 9 (MMP-9).

Refining Risk Stratification for B-cell Precursor Adult Acute Lymphoblastic Leukemia Treated With a Pediatric-inspired Regimen by Combining IKZF1 Deletion and Minimal Residual Disease.

Introduction: Minimal residual disease (MRD) is the most important prognostic factor for B-cell acute lymphoblastic leukemia (B-ALL) however nearly 20-30% of patients relapsed even when they achieved negative MRD, how to identify these patients is less addressed. In this study, we aimed to reassess the prognostic significance of MRD and IKZF1 in adult B-ALL patients receiving pediatric chemotherapy regimens.

Methodology: A total of 202 newly diagnosed adult patients with B-ALL treated at Nanfang Hospital between January 2016 and September 2020 were enrolled in the population-based protocol of the PDT-ALL-2016 trial (NCT03564470), a GRAALL-backbone, peg-aspargase-intensified, antimetabolite-based pediatric-inspired regimen therapy.

Design and preparation of novel magnetic covalent organic framework for the simultaneous preconcentration and sensitive determination of six aflatoxins in food samples.

An innovative core-shell covalent organic framework (COF), FeO@COF (ETTBA-ND), was synthesized through a facile and energy-efficient method. This adsorbent facilitated magnetic solid phase extraction (MSPE) of six AFs prior to LC-MS/MS analysis, achieving one-step purification and enrichment in food matrices. The successful synthesis of the adsorbent was confirmed using various techniques, with adsorption capacities ranging from 46.

Covalent triazine based organic polymers modified ionization source for rapid and sensitive analysis of phytoestrogens.

The monitoring of phytoestrogen levels in food is essential for ensuring food safety; however, the low concentrations and complex matrices pose significant technical challenges. In this study, covalent triazine based organic polymers (TCOPs) was innovatively synthesized in situ on the surface of a steel substrate (TCOPs-S). These novel TCOPs-S were utilized as adsorbents for solid-phase microextraction and ionization sources in ambient mass spectrometry, enabling the rapid detection of phytoestrogens.

Effects of fermented tea by Aspergillus cristatus on the characteristic aroma and non-volatile components of Jiang-flavor baijiu.

Background: Fu Brick tea (FBT) extract has been demonstrated to lower blood lipids, protect liver, and prevent obesity. Despite these benefits, there are no products on the market that combine FBT with other foods or beverages.

Result: In this study, we developed a novel product by combining FBT with high-alcohol Jiang-flavor baijiu, resulting in FBT-baijiu.

Dysregulation of Fatty Acid Metabolism in Breast Cancer and Its Targeted Therapy.

Breast cancer has become the number one cancer worldwide, there are challenges in its prevention, diagnosis and treatment, especially the pathogenesis of triple negative breast cancer has not been clear and the treatment dilemma of metastatic breast cancer. Metabolic reprogramming is currently considered to be one of the hallmarks of cancer, and metabolic alterations in breast cancer, including enhanced glycolysis, tricarboxylic acid cycle activity, glutamine catabolism, and fatty acid biosynthesis, are manifested differently in different breast cancer subtypes and have a complex relationship with tumor growth, metastasis, death, and drug resistance. At present, inhibitors of fatty acid synthesis and oxidation related enzymes have a certain effect in the treatment of breast cancer.

Synthesis of β-cyclodextrin magnetic-graphene-oxide pyrenyl (β-mGOP) gels for high-throughput enrichment and detection of various trace mycotoxins in food using UHPLC-cIMS.

In this research, β-cyclodextrin magnetic-graphene-oxide pyrenyl gel composite (β-mGOP) was employed to remove trace mycotoxins from food products. The β-mGOP gels exhibited a three-dimensional macroporous structure that facilitated the rapid diffusion of target molecules into the nanoporous structure of the covalent organic frameworks embedded within the gel. The adsorption process of mycotoxins onto the β-mGOP gels conformed to the pseudo-second-order kinetics and the Freundlich adsorption model.

Methylated magnetic covalent organic framework for sample preparation and LC-MS/MS detection of 12 tadalafil analogs in dietary supplements.

Tadalafil analogs are often illegally added to dietary supplements such as herbal beverages, protein powders and tablet foods. Due to the complexity of the matrices, effective extraction of tadalafil analogs is the key to achieve accurate quantification. Therefore, it is of great significance to establish a rapid and effective method for the analytical determination of tadalafil analogs in complex matrices.

Dual-use ionization source for direct and sensitive detection of trace carbamates residue.

Directly determining trace compounds in actual sample by ambient ionization mass spectrometry (AIMS) has always been a challenge. Due to the excellent adsorption and desorption properties of nanomaterials, AIMS combined with nanomaterials can improve the adsorption and desorption properties of MS. In this work, we developed a molecularly imprinted graphene oxide coated steel sheet (MIGOCS) to be used as a solid phase extraction adsorbent.

A butyrolactone derivative from marine-derived fungal strain BTBU20211037.

The secondary metabolites of marine-derived fungal strain, BTBU20211037, isolated from Qinhuangdao coast were investigated. Thirteen compounds were isolated and identified, including one new compound, butyrolactone J (), and twelve known compounds, butyrolactone I (), butyrolactone VI (), aspernolide B (), aspernolide A (), 7'-hydroxybutyrolactone III (), methyl asterrate (), methyl dichloroasterrate (), sulochrin (), methyl 6-acetyl-4-methoxy-5,7,8-trihydroxynaphthalene-2-carboxylate (), serantrypinone (), alantrypinone (), and territrem A (). The structures of the compounds were elucidated by HRESIMS, NMR, and ECD analysis.

Asperinsuterpenes A-C from the Fungus BTBU20220155.

Three new meroterpenoids, asperinsuterpenes A-C (-), and eight previously reported natural products, namely asnovolin I (), (2',4',6')-6-(1'-carboxyocta-2',4',6'-triene)-9-hydroxydrim-7-ene-11,12-olide (), (2',4',6')-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene (), cinereain (), carnequinazolines A and B ( and ), carnemycin B (), and stromemycin () were isolated from the fungus strain BTBU20220155. The structures of the compounds were determined based on spectroscopic techniques, including 1D and 2D NMR, HRESIMS, and ECD experiments. The in vitro antimicrobial evaluation revealed that compounds and exhibited inhibitory activity against , with minimum inhibitory concentration (MIC) values of 12.

Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice.

Tranexamic acid (TXA) is widely used among young women because of its ability to whiten skin and treat menorrhagia. Nevertheless, its potential effects on oocyte maturation and quality have not yet been clearly clarified. Melatonin (MT) is an endogenous hormone released by the pineal gland and believed to protect cells from oxidative stress injury.

New Secondary Metabolites from Marine-Derived Fungus BTBU20220184.

Six new compounds, talamitones A and B ( and ), demethyltalamitone B (), talamiisocoumaringlycosides A and B ( and ), and talaminaphtholglycoside (), together with six known compounds (-), were isolated from the marine-derived fungus BTBU20220184. The new structures were characterized by using HRESIMS and NMR. This is the first report of isocoumaringlycoside derivatives from a fungus of the genus.

Secondary Metabolites from Marine-Derived Fungus BTBU20213035.

Two new polyketide derivatives, penirubenones A and B ( and ), and two naturally rare amino-bis-tetrahydrofuran derivatives, penirubenamides A and B ( and ), together with nine known compounds (-) were isolated from the marine-derived fungus BTBU20213035. The structures were identified by HRESIMS and 1D and 2D NMR analyses, and their absolute configurations were determined by a comparison of experimental and calculated electronic circular dichroism (ECD) spectroscopy and C NMR data. We found that exhibited antibacterial activity against , with an MIC value of 3.

Relationship of lncRNA FTX and miR-186-5p levels with diabetic peripheral neuropathy in type 2 diabetes and its bioinformatics analysis.

Background: Diabetic peripheral neuropathy (DPN) frequently occurs as a secondary condition in individuals with type 2 diabetes mellitus (T2DM).

Objective: To explore the relationship of lncRNA FTX and miR-186-5p levels with DPN in T2DM.

Methods: The study enrolled 50 patients with T2DM and 45 patients with DPN.

Triterpenoids with α-glucosidase inhibitory and cytotoxic activities from the fruits of Schisandra chinensis.

An intensive phytochemical investigation into the fruits of Schisandra chinensis afforded 28 triterpenoids incorporating diverse backbones with methyl-migration, ring-expansion and ring-opening features. Among them, ten compounds (1-10) including three likely extracting artefacts (8-10) were described for the first time. Their structures were fully characterized by comprehensive spectroscopic analyses, with the absolute configurations established via electronic circular dichroism and Mosher's NMR techniques.

A Novel Multiarmed Bifunctional PEG Derivative for the Preparation of Mass Spectrometry Ion Sources with Antifouling Property and High Selectivity.

It is challenging to prepare a highly selective mass spectrometry (MS) ion source for the rapid and highly sensitive detection of analytes, especially mycotoxins. In this study, an amino and tetrazine bifunctionalized multiarm PEG derivative (NHHCl-4armPEG10K-(MTz)), which can be easily immobilized on the substrate by the addition reaction between amino and polydopamine, was used for the preparation of MS ionization substrate. NHHCl-4armPEG10K-(MTz) can also be used as a linker to immobilize sufficient streptavidin (SA) on the surface of the substrate by a click reaction.

Preparation of immunomagnetic composite nanostructures with bifunctional four-arm PEG derivatives as linkers for the ultrafast enrichment of zearalenone and its metabolites.

It is challenging to prepare sample pretreatment materials with simple use, strong selectivity and satisfactory enrichment performance. In this study, the antibody (3D4) that can specifically recognize zearalenone (ZEN) and its metabolites was immobilized on the surface of gold-coated magnetic FeO nanoparticles (GMN) by streptavidin (SA)-biotin interaction using GMN as the substrate and our designed four-arm PEG derivative (HS-4ARMPEG10K-(CM)) as the linker. The immunomagnetic nanoparticles (GMN-4ARMPEG10K-SA-3D4) prepared by this strategy can achieve rapid enrichment (only 5 min) of analytes directly in the matrix, and higher enrichment capacity compared with the previous immunomagnetic particles.

STAT5 phosphorylation plus minimal residual disease defines a novel risk classification in adult B-cell acute lymphoblastic leukaemia.

The dysregulation of the Janus family tyrosine kinase-signal transducer and activator of transcription (JAK-STAT) is closely related to acute lymphoblastic leukaemia (ALL), whereas the clinical value of phosphorylated STAT5 (pSTAT5) remains elusive. Herein we performed a prospective study on clinical significance of flow cytometry-based pSTAT5 in adult B-ALL patients. A total of 184 patients were enrolled in the Precision-Classification-Directed-Target-Total-Therapy (PDT)-ALL-2016 cohort between January 2018 and December 2021, and STAT5 phosphorylation was detected by flow cytometry at diagnosis.

A numerical study on the siphonic effect of enhanced external counterpulsation at lower extremities with a coupled 0D-1D closed-loop personalized hemodynamics model.

Enhanced external counterpulsation (EECP) is a treatment and rehabilitation approach for ischemic diseases, including coronary artery disease. Its therapeutic benefits are primarily attributed to the improved blood circulation achieved through sequential mechanical compression of the lower extremities. However, despite the crucial role that hemodynamic effects in the lower extremity arteries play in determining the effectiveness of EECP treatment, most studies have focused on the diastole phase and ignored the systolic phase.

Mpox virus Clade IIb infected Cynomolgus macaques via mimic natural infection routes closely resembled human mpox infection.

Generating an infectious non-human primate (NHP) model using a prevalent monkeypox virus (MPXV) strain has emerged as a crucial strategy for assessing the efficacy of vaccines and antiviral drugs against human MPXV infection. Here, we established an animal model by infecting cynomolgus macaques with the prevalent MPXV strain, WIBP-MPXV-001, and simulating its natural routes of infection. A comprehensive analysis and evaluation were conducted on three animals, including monitoring clinical symptoms, collecting hematology data, measuring viral loads, evaluating cellular and humoral immune responses, and examining histopathology.

Comparison of genomic alterations in Epstein-Barr virus-positive and Epstein-Barr virus-negative diffuse large B-cell lymphoma.

Background: Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (EBV-posDLBCL) is an aggressive B-cell lymphoma that often presents similar morphological and immune phenotype features to that of EBV-negative DLBCL (EBV-negDLBCL).

Aims And Methods: To better understand their difference in genomic landscape, we performed whole-exome sequencing (WES) of EBV-posDLBCL and EBV-negDLBCL.

Results: This analysis revealed a new mutational signature 17 (unknown) and signature 29 (smoking) in EBV-posDLBCL as well as a specific mutational signature 24 (associated with aflatoxin) in EBV-negDLBCL.