Stability of diazepam's phase II metabolites in dried blood spots on filter paper.

Unlabelled: Phase II metabolites play an important role in diazepam-related cases. The study aimed to assess the stability of diazepam's phase II metabolites in dried blood spots on filter paper.

Methods: A piece of filter paper was spotted with 100 µL of whole blood (added 1% sodium fluoride as needed) obtained from participant who received 5 mg diazepam orally, air dried for 2 h at room temperature, and then stored at different conditions.

Simulating dynamic interaction between diazepam and ethanol targeting the GABA receptor via in silico model.

Combined diazepam-ethanol poisoning is common in forensic toxicology. Both diazepam and ethanol can inhibit the central nervous system via the γ-aminobutyric acid (GABA) ligand gated chloride ion channel, GABA Receptor (GABAR). As the common target of diazepam and ethanol, whether GABAR is the key target of their combined action remains unclear.