The Chromosome-Scale Genome of Magnolia sieboldii K. Koch Provides Insight Into the Evolutionary Position of Magnoliids and Seed Germination.

Magnolia sieboldii K. Koch (M. sieboldii) stands as an elegant tree species within the Magnoliaceae family, esteemed for its exquisite beauty, cultural significance and economic advantages.

Identification and Functional Analysis of V-ATPaseA and C Genes in .

Vacuolar (H)-ATPases (V-ATPases) are ATP-driven proton pumps that play multifaceted roles across various organisms. Despite their widespread significance, the functional implications of genes in , an invasive forest pest with a global presence, have yet to be elucidated. In this study, two specific genes from were identified and analyzed, namely (accession number: OR217451) and (accession number: OR217452).

Novel Environmentally Friendly RNAi Biopesticides: Targeting in Larvae Using Layered Double Hydroxide Nanocomplexes.

RNA interference (RNAi)-based biopesticides offer an attractive avenue for pest control. Previous studies revealed high RNAi sensitivity in larvae, showcasing its potential for grub control. In this study, we aimed to develop an environmentally friendly RNAi method for larvae.

Identification of Functional Brassinosteroid Receptor Genes in Oaks and Functional Analysis of .

Brassinosteroids (BRs) play important regulatory roles in plant growth and development, with functional BR receptors being crucial for BR recognition or signaling. Although functional BR receptors have been extensively studied in herbaceous plants, they remain largely under-studied in forest tree species. In this study, nine BR receptors were identified in three representative oak species, of which BRI1s and BRL1s were functional BR receptors.

Composition and Diversity of Endophytic Rhizosphere Microbiota in Apple Tree with Different Ages.

In order to determine the underlying mechanism of the senescence occurring in older apple trees, the effects of tree age on the community structure and dominant genus of endophytic rhizosphere bacteria in apple were investigated. The diversity and structure of the bacterial communities and corresponding changes in the dominant genera of endophytic rhizosphere bacteria of apple at different ages (2, 8, 16, 22 years) were compared based on 16S rRNA high-throughput sequencing technology. The results revealed that the longer the tree age, the less the number of ASV in the endophytic bacteria.

Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, .

Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects. Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm larvae. The cDNAs of contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.

Genome-Wide Identification and Expression Analysis of the bHLH Transcription Factor Family and Its Response to Abiotic Stress in Mongolian Oak ().

The basic helix-loop-helix (bHLH) family, one of the largest families of transcription factors in plants, is extensively involved in the growth, development, and stress response of several woody plants. However, no systematic analysis of the bHLH gene family in has been reported. We characterize genes and identify the functions of QmbHLH proteins in .

Identification and functional analysis of two potential RNAi targets for chitin degradation in Holotrichia parallela Motschulsky (Insecta Coleoptera).

Chitin metabolism enzymes are safe and desirable targets for pest management. β-N-acetylglucosaminidase (NAG) and N-acetylglucosamine kinase (NAGK) are involved in chitin degradation. NAG is the main glycosidase that works synergistically with chitinases.

Transcriptomic insights into the effects of abscisic acid on the germination of Magnolia sieboldii K. Koch seed.

Magnolia sieboldii K. Koch is a deciduous tree species. However, the wild resource of M.

Genome-wide identification of the auxin response factor (ARF) gene family in and functional analysis of .

Auxin plays an essential role in flowering, embryonic development, seed dormancy, and germination. Auxin response factors (ARFs) are plant-specific key transcriptional factors in mediating the gene expression network of auxin signaling. Although ARFs in model plants such as had been well characterized, their identities and potential roles in non-model plants are less studied.

[Responses of stand growth, regeneration, and understory species diversity in secondary forest to stand density].

Six plots with an area of 0.1 hm were thinned in 2018. A field survey was carried out in 2020 to examine the effects of different stand densities (high: 900 trees·hm; medium: 720 trees·hm; low: 600 trees·hm) on growth and regeneration of stands and understory species diversity of secondary .

Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.

Quercus mongolica Fisch. ex Ledeb is the main species of coniferous and broadleaved mixed forests in northeast and north China, which has high ornamental, economic, and ecological value. The appropriate reference genes must be selected for quantitative real-time PCR to reveal the molecular mechanisms of stress responses and their contribution to breeding of Q.

A chromosome-scale genome assembly of the Mongolian oak (Quercus mongolica).

Mongolian oak (Quercus mongolica Fisch.) is an ecologically and economically important white oak species native to and widespread in the temperate zone of East Asia. Here, we present a chromosome-scale reference genome assembly of Q.

Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Maxim.

Maxim is a hermaphroditic plant belonging to the genus in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male flowers bloom first.

Cloning and expression analyses of a gene from K. Koch.

K. Koch is endemic to China and has high medicinal and ornamental values. However, its seed exhibits morphophysiological dormancy, and the molecular mechanisms of which are not clearly understood.

Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination.

Magnolia sieboldii K. Koch (M. sieboldii) is a deciduous Chinese tree species of the Magnoliaceae family with high ornamental, medicinal, and economic benefits.

Flower Development of Heterodichogamous (Juglandaceae).

is a monoecious heterodichogamous species with protogynous and protandrous mating strategies that occur at a 1:1 ratio and are randomly distributed in the population. The inconsistent male and female flowering periods of the same mating type result in an imbalance of the ratio of male and female flowers, contributing to the low yield of this species. However, little more is known about its floral development.

RNA interference-mediated functional characterization of Group I chitin deacetylases in Holotrichia parallela Motschulsky.

Chitin deacetylases (CDAs, EC 3.5.1.

Transcriptome Analysis of Two Different Developmental Stages of Seeds.

is a herbaceous flower in the family with both hypocotyl and epicotyl dormant seeds. We used high-throughput transcriptome sequencing on two different developmental stages of seeds to identify seed dormancy and germination-related genes. We performed de novo assembly and annotated a total of 123,577 unigenes, which encoded 24,688 putative proteins with 47 GO categories.

Functions of microRNA in seed development, dormancy and germination processes.

In plants, microRNAs (miRNAs) are a class of non-coding small RNAs that play important roles in various biological processes, such as seed development, dormancy and germination. In these stages, seeds are regulated by various extrinsic environmental factors and intrinsic hormones. Different species of seeds have distinct development and dormancy characteristics.

Proteomics profiling of ethylene-induced tomato flower pedicel abscission.

Unlabelled: The control of abscission is an important agricultural concern because of its substantial effect on crop yield and quality. Changes in gene expression are correlated with the ethylene-mediated execution of abscission. However, only few large-scale proteomic studies focused on tomato pedicel abscission.

Identification of a new peritrophic membrane protein from larval Holotrichia parallela (Coleoptera: Motschulsky).

Peritrophic membranes (PMs) are composed of proteins, proteoglycans and chitin that play important roles in the structural formation and function of the PM. This study identified and characterized a new chitin binding protein named HpCBP45 by immunoscreening of the Holotrichia parallela larvae midgut expression library. The predicted amino acid sequence indicates that it contains eight tandem chitin binding domains belonging to the peritrophin-A family.

[Variations of arsenic species in the solution of arsenic-contaminated paddy soil under flooding and at different temperatures].

Taking arsenic (As)-contaminated paddy soil as test object, and by using high performance liquid chromatography inductively coupled plasma-mass spectrometry (HPLC-ICP-MS), this paper studied the variations of As species in soil solution when the soil was sterilized or non-sterilized and incubated at different temperatures (5, 27, and 50 degrees C) under flooding. In the soil solution (pore water), only As(III) (arsenite), As(V) (arsenate), and DMA(V) ( dimethylarsinic acid) were detected, but no MMA(V)(mono methylarsinic acid) was found. With the increasing time of flooding and at the test temperatures, arsenite became the predominant species, averagely accounting for 64%, followed by As(V), with the proportion of 35%, and DMA(V), with the least proportion of 1%.