Development of a 17-plex STR typing system for the identification of individuals and parentage testing in cattle.

Accurate identification of animals and the verification of their parentage can be used to pedigree populations and support selective breeding. The International Society for Animal Genetics recommended 16 cattle STRs for individual identification and parentage testing in cattle, but no multiplex STR typing system contains these 16 STRs. Here, we develop an efficient 17-plex multiplex typing system for cattle that contains the 16 ISAG recommend STRs and a sex-determining marker.

The Non-Denatured Processing of Mucilage-Characteristics of Hydrodynamic Properties and the Effect on In Vivo Functions.

Food non-denatured processes, such as freeze-drying and grinding, are commonly applied to raw materials with good bioactive functions. Although the functional components are maintained, whether structural and physical changes impact the in vivo function is often ignored in practical situations. mucilage (BSM) has a significant alleviation effect on DSS-induced colitis.

Automatic PCB Sample Generation and Defect Detection Based on ControlNet and Swin Transformer.

In order to improve the efficiency and accuracy of multitarget detection of soldering defects on surface-mounted components in Printed Circuit Board (PCB) fabrication, we propose a sample generation method using Stable Diffusion Model and ControlNet, as well as a defect detection method based on the Swin Transformer. The method consists of two stages: First, high-definition original images collected in industrial production and the corresponding prompts are input to Stable Diffusion Model and ControlNet for automatic generation of nonindependent samples. Subsequently, we integrate Swin Transformer as the backbone into the Cascade Mask R-CNN to improve the quality of defect features extracted from the samples for accurate detection box localization and segmentation.

Proteomics Analysis Reveals the Underlying Factors of Mucilage Disappearance in and Its Influence on Nutrient Accumulation.

J.F. Gmel (BS) is rich in mucilage, which has diverse biological activities, and is utilized in the food and pharmaceutical industries due to its nutritional value.

A special polysaccharide hydrogel coated on : preventive effects against ulcerative colitis modulation of gut microbiota.

Ulcerative colitis (UC) is a growing health concern in humans, but it can be prevented by using special dietary strategies. Young stems and leaves of (BS) are coated with a special polysaccharide hydrogel (BS mucilage) which can be beneficial for colon health. The aim of this study was to investigate the preventive effects of BS mucilage against UC in a DSS-treated mouse model.

Psychometric evaluation of the Wound-QoL questionnaire to assess health-related quality of life in Chinese people with chronic wounds.

Chronic wounds are very common wound types in clinics which have a prolonged and painful healing process. Chronic wounds affect health-related quality of life (HRQoL) on patients. However, there is no specific instrument to measure the HRQoL in Chinese patients with chronic wounds.

Inhibition of S100A8/A9 ameliorates renal interstitial fibrosis in diabetic nephropathy.

Background: Renal interstitial fibrosis (RIF) is one of the main features of diabetic nephropathy (DN), but the molecular mechanisms mediating RIF in DN has yet been fully understood. S100A8 and S100A9 are the proteins associated with immune and inflammation response. Here we reported the expression of S100A8 and S100A9 were significantly increased on tubular epithelial cells in diabetic kidneys through a proteomic analysis.

Quercetin Attenuates Podocyte Apoptosis of Diabetic Nephropathy Through Targeting EGFR Signaling.

Podocytes injury is one of the leading causes of proteinuria in patients with diabetic nephropathy (DN), and is accompanied by podocytes apoptosis and the reduction of podocyte markers such as synaptopodin and nephrin. Therefore, attenuation of podocyte apoptosis is considered as an effective strategy to prevent the proteinuria in DN. In this study, we evaluated the anti-podocyte-apoptosis effect of quercetin which is a flavonol compound possessing an important role in prevention and treatment of DN and verified the effect by using mice and high glucose (HG)-induced mouse podocytes (MPs).

Novel Immune-Related Genetic Expression for Primary Sjögren's Syndrome.

To identify novel immune-related genes expressed in primary Sjögren's syndrome (pSS). Gene expression profiles were obtained from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were screened. The differences in immune cell proportion between normal and diseased tissues were compared, weighted gene co-expression network analysis was conducted to identify key modules, followed by a protein-protein interaction (PPI) network generation and enrichment analysis.

Sarsasapogenin restores podocyte autophagy in diabetic nephropathy by targeting GSK3β signaling pathway.

Podocyte injury following abnormal podocyte autophagy plays an indispensable role in diabetic nephropathy (DN), therefore, restoration of podocyte autophagy is considered as a feasible strategy for the treatment of DN. Here, we investigated the preventive effects of sarsasapogenin (Sar), the main active ingredient in Anemarrhena asphodeloides Bunge, on the podocyte injury in diabetic rats, and tried to illustrate the mechanisms underlying the effects in high glucose (HG, 40 mM)-treated podocytes (MPs). Diabetes model was established in rats with single streptozocin (60 mg· kg) intraperitoneal administration.

miR-642 serves as a tumor suppressor in hepatocellular carcinoma by regulating SEMA4C and p38 MAPK signaling pathway.

Hepatocellular carcinoma (HCC) is a malignant tumor with high incidence and high risk. Study of the role and mechanism of miRNAs are a hot spot of research providing new treatment ideas in malignant tumors. The effect of miR-642a on HCC progression and the underlying molecular mechanism were investigated.

Preparation of Pt/γ-BiMoO Photocatalysts and Their Performance in α-Alkylation Reaction under Visible Light Irradiation.

Bi(NO)·5HO and (NH)MoO·4HO were used as precursors to synthesize flaky γ-BiMoO samples by a hydrothermal method, and Pt/γ-BiMoO samples with different mass fractions were prepared by an NaBH reduction method. Alpha alkylation of benzyl alcohol and acetophenone with photocatalysts under visible light irradiation was performed, and the activity of 4 wt % Pt/γ-BiMoO (γ-BiMoO was prepared by a nitric acid method, pH = 9, and reaction temperature 180 °C) was the best. The photocatalytic reaction conditions were optimized by changing various kinds of variables, such as the type of catalyst, solvent, and base, and the amount of base, catalyst, and reactant.

Fiber birefringence measurement by an external applied strain method and a polarimetric fiber laser sensor.

In this work, a high-sensitivity and low-cost sensing scheme for measuring intrinsic and induced fiber birefringence change is reported based on a polarimetric fiber laser sensor interrogated by the beat frequency technique. The fiber birefringence measurement is achieved by an external applied strain method. A detailed theoretical analysis of the principle for fiber birefringence measurement is carried out.

Hybrid wavelength- and frequency-division multiplexed fiber laser sensor array.

The Letter describes a hybrid multiplexing scheme for a multi-longitudinal-mode fiber laser sensor array by combining wavelength-division multiplexing and frequency-division multiplexing techniques. The proposed multiplexing system consists of many subgroups, each of which has many fiber laser sensors with approximately equal laser-operating wavelengths but different cavity lengths. Each sensor in one subgroup can be interrogated by using frequency-division multiplexing technology because of different sensors with different beat frequencies.

Employment of Near Full-Length Ribosome Gene TA-Cloning and Primer-Blast to Detect Multiple Species in a Natural Complex Microbial Community Using Species-Specific Primers Designed with Their Genome Sequences.

It remains an unsolved problem to quantify a natural microbial community by rapidly and conveniently measuring multiple species with functional significance. Most widely used high throughput next-generation sequencing methods can only generate information mainly for genus-level taxonomic identification and quantification, and detection of multiple species in a complex microbial community is still heavily dependent on approaches based on near full-length ribosome RNA gene or genome sequence information. In this study, we used near full-length rRNA gene library sequencing plus Primer-Blast to design species-specific primers based on whole microbial genome sequences.

Study on preparation of SnO-TiO/Nano-graphite composite anode and electro-catalytic degradation of ceftriaxone sodium.

In order to improve the electro-catalytic activity and catalytic reaction rate of graphite-like material, Tin dioxide-Titanium dioxide/Nano-graphite (SnO-TiO/Nano-G) composite was synthesized by a sol-gel method and SnO-TiO/Nano-G electrode was prepared in hot-press approach. The composite was characterized by X-ray photoelectron spectroscopy, fourier transform infrared, Raman, N adsorption-desorption, scanning electrons microscopy, transmission electron microscopy and X-ray diffraction. The electrochemical performance of the SnO-TiO/Nano-G anode electrode was investigated via cyclic voltammetry and electrochemical impedance spectroscopy.

Preparation and characterization of Nano-graphite/TiO2 composite photoelectrode for photoelectrocatalytic degradation of hazardous pollutant.

Nano-graphite(Nano-G)/TiO2 composite photoelectrode was fabricated via sol-gel reaction, followed by the hot-press approach. The morphology, structure and light absorption capability of composite was characterized by various characterizations. The photoelectrochemical property and photoelectrocatalytic(PEC) activity of photoelectrode were also investigated.

Fabrication of 3 D Mesoporous Black TiO2 /MoS2 /TiO2 Nanosheets for Visible-Light-Driven Photocatalysis.

A novel 3 D mesoporous black TiO2 (MBT)/MoS2 /MBT sandwich-like nanosheet was successfully fabricated using a facile mechanochemical process combined with an in situ solid-state chemical reduction approach, followed by mild calcination (350 °C) under an argon atmosphere. The MBT/MoS2 /MBT exhibits a 3 D sandwich-like nanosheet structure and heterojunctions are formed at the interfaces between MoS2 and black TiO2 . The significantly narrowed band gap of MBT/MoS2 /MBT is attributed to the introduction of MoS2 and the formed Ti(3+) species in the frameworks.

Preparation for CeO2/Nanographite Composite Materials and Electrochemical Degradation of Phenol by CeO2/Nanographite Cathodes.

CeO2/nanographite (CeO2/nano-G) composite materials were got by chemical precipitation method with nanographite (nano-G) and cerous nitrate hexahydrate as raw materials. The microstructures of CeO2/nano-G composite materials were characterized by means of SEM, XRD, XPS and Raman. The cathodes were made by nano-G and CeO2/nano-G composite materials, respectively.

Preparation for Mn/nanographite materials and study on electrochemical degradation of phenol by Mn/nanographite cathodes.

Mn/nanographite (nano-G) materials were got by chemical redox reaction and using nano-G, potassium permanganate and manganese acetate as raw materials. The microstructures and properties of nano-G and Mn/nano-G sheets were characterized by means of SEM, XPS, XRD and Raman. The results showed that manganese oxide nanoscale rod inlaid on the graphite layer surface, the manganese valence of Mn/nano-G was +4 and existed in the form of the mix crystal of α-MnO2 and γ-MnO2.

Effect of post-annealing treatment on photocatalytic and photoelectrocatalytic performances of TiO2 nanotube arrays photoelectrode.

Highly ordered TiO2 nano-tube arrays (TNTAs) photoelectrodes were prepared through anodization method, followed by annealing treatment. Morphologies and structures of the as-prepared TNTAs samples were investigated through scanning electron microscopy (SEM) and X-ray diffraction (XRD). In addition, the optical and photoelectrochemical (PECH) properties were investigated through ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS) and transient photocurrent response.

Spatial variation and subcellular binding of metals in oysters from a large estuary in China.

Pearl River Estuary (PRE) is the largest estuary in Southern China and there has been an increasing concern of metal pollution due to regional industrialization. In this study, we investigated the spatial variation of metal pollution (Ag, As, Cd, Cu, Pb, and Zn) as well as their subcellular handling in the oyster Crassostrea hongkongensis. Hot spots of metal contamination in the oysters were found in different sites, suggesting that there were different sources of metals in the estuary associated with industrial activity.

Mercury distribution, speciation and bioavailability in sediments from the Pearl River Estuary, Southern China.

Surface sediments and sediment cores collected from the Pearl River Estuary (PRE) were analyzed for total mercury (THg) concentrations and speciation using a sequential extraction method. The mobility of Hg in sediments was also assessed using a series of single extraction methods. The surface sediments from the PRE showed slightly elevated levels of Hg, with concentrations ranging from 109 to 453 ng/g.