Hydrogen sulfide (HS) has been identified as a novel gasotransmitter and a substantial antioxidant that can activate various cellular targets to regulate physiological and pathological processes in mammals. However, under physiological conditions, it remains unclear whether it is involved in regulating cardiomyocyte (CM) proliferation during postnatal development in mice. This study mainly aimed to evaluate the role of HS in postnatal CM proliferation and its regulating molecular mechanisms.
View Article and Find Full Text PDFStem Cell Rev Rep
February 2024
Stem cell therapy holds great promise for future clinical practice for treatment of advanced liver diseases. However, the fate of stem cells after transplantation, including the distribution, viability, and the cell clearance, has not been fully elucidated. Herein, recent advances regarding the imaging tools for stem cells tracking mainly in chronic liver diseases with the advantages and disadvantages of each approach have been described.
View Article and Find Full Text PDFAllergol Immunopathol (Madr)
November 2023
Objective: To detect serum metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases (TIMP-1), cyclooxygenase-2 (COX-2), and T helper cells 1-T helper cells 2 (Th1-Th2) levels in asthma patients and assess their clinical significance.
Methods: A total of 72 patients experiencing acute asthma (acute group), 66 stable asthma patients (stable group), and 60 healthy volunteers (control group) were included in this study. The levels of TIMP-1, COX-2, and Th1-Th2 in patients with acute asthma were measured following treatment with budesonide aerosol inhalation.
Background: Uricase (Uox) is a major drug in gout and a supplementary drug in cancer treatment. Because allergic reactions caused by Uox limit its clinical application,10% Co/EDTA was used to chemically modify Uox from A. flavus to reduce its immunogenicity.
View Article and Find Full Text PDFThe interaction mechanism between carbon dots (CDs) and metal ions is essential for optimizing their design, synthesis, and application. However, it must be accurately distinguished and quantified because of CDs' complex structure, composition, and coexisting various response mechanisms or products. Herein, a recirculating-flow fluorescence capillary analysis (RF-FCA) system was developed to online monitor the fluorescence kinetics of CDs interacting with metal ions.
View Article and Find Full Text PDFCascade reactions catalyzed by natural uricase and mimic peroxidase (MPOD) have been applied for uric acid (UA) detection. However, the optimal catalytic activity of MPOD is mostly in acidic conditions (pH 2-5), mismatching the optimal catalytic alkaline environment of uricase. In this paper, using CuSO and urea as raw materials, a MPOD with high catalytic activity in alkaline environment was synthesized by hydrothermal method.
View Article and Find Full Text PDFIn using a flow-injection recirculating-catalysis system developed by us to research the simultaneous quantification for peroxidase and ascorbate, it was discovered that the concentrations of peroxidase activity and ascorbate are correlative with the slope and the negative intercept of the linear response curve during a peroxidase-catalyzed kinetic course. Therefore, based on this finding, a new analytical method and a simplified equation for quantifying the peroxidase activity concentration were proposed, Then, test conditions were optimized, finally the use of the method has realized the simultaneous determination for peroxidase of 2-40 U/L and ascorbate of 0.4-12 mg/L in various vegetables (60 μL).
View Article and Find Full Text PDFSickle cell disease (SCD) is characterized by frequent and unpredictable vaso-occlusive crises (VOCs). Sickle erythrocytes (SSRBCs) contribute to VOCs by participating in a series of adhesive events with blood cells and the vascular endothelium. Adhesion assays have been used to evaluate the relationship between SSRBC adhesion and SCD severity.
View Article and Find Full Text PDFBlood cell adhesion to P-selectin and vascular cell adhesion molecule-1 (VCAM-1) contributes to the pathophysiology of vaso-occlusion crisis (VOC) events in individuals with sickle cell disease (SCD). We evaluated the use of standardized flow adhesion biomarkers in a six-month, 35-subjects longitudinal study (ELIPSIS). Flow adhesion of whole blood on P-selectin (FA-WB-Psel) and VCAM1 (FA-WB-VCAM), and of isolated white blood cells on P-selectin (FA-WBC-Psel) and VCAM-1 (FA-WBC-VCAM) were elevated on VOC days compared with non-VOC days, but only FA-WB-Psel reached statistical significance (P = 0·015).
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
December 2021
A new fluorescence probe method for the detection of Hg(II) in serum was established, which has the detection limit of 3.57 nM and quantification limit of 5 nM, based on the electrostatic induced agglomeration quenching and complexation between Hg(II) and silicon-nitrogen-doped carbon nanodots (Si/N-CDs). Furthermore, the fluorescence probe also showed the satisfactory results in the determination of Hg(II) in human serum.
View Article and Find Full Text PDFHerein, cobalt and nitrogen co-doped carbon dots (Co-N-CDs) were fabricated via a one-pot hydrothermal approach. The obtained Co-N-CDs displayed peroxidase-like activity and fluorescence properties. It could catalyze the oxidization of guaiacol (GA) in the presence of hydrogen peroxide (HO), and thus, resulted in color change, accompanied by a new absorption peak in 470 nm.
View Article and Find Full Text PDFSichuan Da Xue Xue Bao Yi Xue Ban
January 2021
Objective: R6G-ddATP was used as a dideoxy fluorescence substrate to establish the single base end extension (SNaPShot)-gel fluorescence method for the rapid detection of the genotypes of three high-risk human papillomaviruses (HR-HPV) ( 18, 33 and 35) genotypes.
Methods: HPV quality control products were used as as samples, and R6G-ddATP dideoxy fluorescence reagent was used as substrate. Firstly, HPV was amplified by using universal primers to obtain the first round of amplified products, which were purified and used as templates for subsequent SNaPShot reactions.
Clin Hemorheol Microcirc
February 2022
Background: Red blood cell (RBC)-modifying therapies have provided new opportunities for patients with sickle cell disease, although the absence of validated biomarkers of RBC function is a barrier to FDA approval and clinical adoption. Flow Adhesion (FA) and Mechanical Fragility (MF) biomarkers objectively stratify individuals with SCD into pro-adhesive vs pro-hemolytic phenotypes respectively, which may potentially help predict therapeutic responses.
Objective: A Phase 3 clinical trial to determine the effectiveness of vepoloxamer, an RBC-modifying therapy in sickle cell disease (SCD), failed to meet its primary clinical outcome.
Spectrochim Acta A Mol Biomol Spectrosc
April 2021
In this paper, an ultrasensitive and rapid "turn-on" fluorescence sensor, integrating flow-injection (FI) with nitrogen-doped carbon dots/gold nanoparticles (N-CDs/AuNPs) double-probe is established for the determination of metformin hydrochloride (MET) in biological fluids. The sensing strategy involves the weak inner filter effect between AuNPs and N-CDs due to aggregation products of MET with AuNPs. Unfortunately, the degree of AuNPs aggregation is difficult to control through manual assays, resulting in intolerable measurement error that limits further applications.
View Article and Find Full Text PDFThe use of a recirculating-flow catalysis detection system (RFCD) explored competition and the influence of ascorbic acid (AsA) in peroxidase (POD)-catalyzed reactions. The study identified that AsA is neither the inhibitor of POD nor could directly deplete H2O2; it directly reacts with chromogenic products to form colorless intermediates, which can react with H2O2 to again rapidly re-generate the chromogenic products. If using the reactions (trinder reactions or enzyme-linked reactions) to determine POD activity (EPOD), substrates or analytes, the interference of concomitant AsA should be removed and the conclusions have significance for oxidase/POD catalyzed reactions.
View Article and Find Full Text PDFPeroxidase (POD) and ascorbic acid (AsA) usually coexist in organisms to synergistically protect them from reactive oxygen damage, and their contents undergo dynamic changes under different physiological conditions. What's more, the response of POD-catalytic activity in spectrophotometry has to be corrected using the content of concomitant AsA because we found that there is an extinction reaction between AsA and chromogenic products obtained from POD catalysis. With these implications, by skilfully using the chromogenic and the extinction phenomena in the guaiacol/POD/HO reaction, an automatic analysis system for simultaneous quantification of POD (73-440 U L) and AsA (4-60 mg L) was successfully established based on flow injection analysis (FIA).
View Article and Find Full Text PDFThe research developed a diazo-coupling carbon-dots (CDs) method for determining nitrite and optimized variables of sodium sulfanilate, CDs synthesis, characteristic wavelength, reaction time and temperature. The method can assay 0.025-2.
View Article and Find Full Text PDFFluorescence capillary analysis (FCA) realizes trace-level analysis of micro-volume samples; it is easy to operate, extremely low in analytical cost and can significantly lessen environmental pollution from analytical chemistry waste. FCA has the characteristics of green analytical chemistry and has been applied in clinical, biochemical, pharmaceutical, food safety and other fields. FCA basically involves a micro-volume glass capillary, a capillary holder and an ordinary fluorescence detector.
View Article and Find Full Text PDFSickle cell disease (SCD) is characterized by frequent and unpredictable vaso-occlusive episodes (VOEs) that lead to severe pain, organ damage, and early death. Lack of reliable biomarkers that objectively define VOEs remains a critical barrier to improving the care for SCD patients. VOEs result from a complex interplay of cell-cell interactions that promote micro-vascular occlusion.
View Article and Find Full Text PDFSichuan Da Xue Xue Bao Yi Xue Ban
September 2019
Objective: To establish SNaPShot-fluorescence capillary analysis (SNaPShot-FCA) assay for rapid detection of the genotype of aldehyde dehydrogenase 2 gene (2) rs671 locus.
Methods: The genomic DNA was extracted from peripheral blood cells. Using R6G-ddATP and cy5-ddGTP as fluorescent substrates, the 2 gene was amplified by SNaPShot to generate DNA products with different fluorescent dyes at the 3' end.
We found that nitrite after protonation can react with amine radical on citric acid/ethylenediamine carbon nanodots (CA/EDA-CDs) to form nitrosamines, and fluorescence quenching of CA/EDA-CDs occurred during this process. Using the reaction mechanism a fluorescence quenching capillary analysis (FQCA) was developed. After optimized reaction conditions, the following results were obtained: the required concentration of CA/EDA-CDs was 12 mg/L, HCl concentration was 32 mmol/L, and the reaction conducted in room temperature for 20 min.
View Article and Find Full Text PDFThe standard method for methanol assay in wine is based on a methanol/KMnO/HCO/fuchsin sodium sulfite (FSS) reaction system. However, it is difficult to control the degree of colour and the temperature of the reaction product in this assay, and its repeatability is also poor due to the generation of CO and CO in the reaction. Therefore, to solve these problems, potassium metabisulfite was selected to replace HCO, and an automatic analysis method was developed which can realize rapid and accurate determination of methanol and can be used to make an online analyzer.
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