[Fluorescence spectra and test application study on the interaction of fluorescein and bovine serum albumin].

A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2.

[Determination of creatine, phosphocreatine and adenosinephosphates in experimental hydrocephalus tissue by reversed-phase high performance liquid chromatography].

Objective: To investigate the state of impaired cerebral energy metabolism of the hydrocephalus tissue.

Methods: Adult male dogs were used for establishing the model of kaolin-induced hydrocephalus. A simple and rapid method for the simultaneous determination of creatine (Cr), phosphocreatine (Crp), and adenosinephosphates (AMP, ADP, ATP) in different stages of experimental hydrocephalus tissue by reversed-phase high performance liquid chromatography (RP-HPLC) has been established.