Chondrogenic differentiation of BMSCs encapsulated in chondroinductive polysaccharide/collagen hybrid hydrogels.

Although BMSC-based therapy is one of the most front-line technologies for cartilage repair, it is still a big challenge to attain ideal niches for BMSC chondrogenic differentiation. In this study, we developed hyaluronate and chondroitin sulfate derivatives to prepare covalently crosslinked polysaccharide hydrogels. Based on these binary hydrogels, collagen was added to prepare ternary hybrid hydrogels and its effect on encapsulated BMSCs was studied.

Extracellular matrix powder from cultured cartilage-like tissue as cell carrier for cartilage repair.

Cartilage extracellular matrix (ECM) is a promising material for cartilage repair because of its bioactivity. However, the animal source of ECM unavoidably increases the risk of pathogen infection and the variability of product quality. In this study, we utilized a novel 3D culture method to prepare a new type of artificial decellularized matrix powder (DEMP) for the development of injectable, bioactive, biodegradable cell carriers for cartilage tissue engineering.

Degradation modeling of mid-power white-light LEDs by using Wiener process.

The IES standard TM-21-11 provides a guideline for lifetime prediction of LED devices. As it uses average normalized lumen maintenance data and performs non-linear regression for lifetime modeling, it cannot capture dynamic and random variation of the degradation process of LED devices. In addition, this method cannot capture the failure distribution, although it is much more relevant in reliability analysis.

Identification of functional cis-elements required for repression of the Taka-amylase A gene under secretion stress in Aspergillus oryzae.

The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, which was depressed under secreted protein stress. We conducted a truncation and deletion analysis of the amyB promoter to identify cis-elements required for RESS.

[One-step fermentation converting glucose in gamma-aminobutyric acid by a recombinant Corynebacterium crenatum].

Objective: We constructed an integrative recombinant Corynebacterium crenatum that could directly convert glucose in gamma-aminobutyric acid (GABA).

Methods: Using overlap PCR, we obtained delta argB fragment that lacked 491 bp of N-acetylglutamate kinase (NAGK) gene. The glutamate decarboxylase (GAD) gene attached tac promoter was amplified and inserted into delta argB fragment.

The biomass and aboveground net primary productivity of Schima superba-Castanopsis carlesii forests in east China.

The biomass and productivity of Schima superba-Castanopsis carlesii forests in Tiantong, Zhejiang Province, were determined using overlapping quadrants and stem analyses. The total community biomass was (225.3+/-30.