Large-capacity DNA vectors based on rolling circle amplification with multivalent aptamers delivery copper sulfide for the synergistic treatment of Cancer through chemo/Photothermal/Chemodynamic therapy in vitro.

Developing multifunctional nanomedicines represents a frontier. We have engineered a high-capacity DNA vector basing rolling circle amplification for the delivery of copper sulfide nanoparticles (CuS NPs) and doxorubicin (DOX), coupled with multivalent aptamers (MA) that precisely target tumors, culminating in a multifunctional nanoplatform (RMALCu@DOX), which combines the chemotherapy (CT)/photothermal therapy (PTT)/chemodynamic therapy (CDT). The vector (RMAL) boasts exceptional biocompatibility and incorporates multiple copy units, enabling the precise loading of numerous CuS NPs, forming RMALCu which possesses a robust photothermal effect and superior Fenton-like catalytic activity, heralding a project of minimally invasive dual-mode (PTT/CDT) therapy.

Versatile adhesive skin enhances robotic interactions with the environment.

Electronic skins endow robots with sensory functions but often lack the multifunctionality of natural skin, such as switchable adhesion. Current smart adhesives based on elastomers have limited adhesion tunability, which hinders their effective use for both carrying heavy loads and performing dexterous manipulations. Here, we report a versatile, one-size-fits-all robotic adhesive skin using shape memory polymers with tunable rubber-to-glass phase transitions.

Integrating L-Cys-AuNCs in ZIF-8 with Enhanced Fluorescence and Strengthened Stability for Sensitive Detection of Copper Ions.

Gold nanoclusters (AuNCs) have been widely investigated because of their unique photoluminescence properties. However, the applications of AuNCs are limited by their poor stability and relatively low fluorescence. In the present work, we developed nanocomposites (L-Cys-AuNCs@ZIF-8) with high fluorescence and stability, which were constructed by encapsulating the water-dispersible L-Cys-AuNCs into a ZIF-8 via Zn-triggered growth strategy without high temperature and pressure.

Discrimination of normal/cancer cells in bioimaging through a rolling circle amplification-enhanced red carbon dots-embedded multivalent aptamers nanoplatform.

Glutathione (GSH) is a key biomarker closely associated with cancer, and its content varies greatly between normal cells and cancer cells. However, intracellular detection of GSH was challenging because existing probes not only have a long detection time but also have fluorescence in the blue-green region that overlaps with the biological matrix's spontaneous fluorescence, thus affecting the detection accuracy. Therefore, a new red fluorescent nano-probe was needed to rapidly and accurately detected GSH within the biological matrix.

Enhancing D-lactic acid production from non-detoxified corn stover hydrolysate via innovative F127-IEA hydrogel-mediated immobilization of T15.

Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.

Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable clusters within the gel matrix.

Engineering a Binding Peptide for Oriented Immobilization and Efficient Bioelectrocatalytic Oxygen Reduction of Multicopper Oxidases.

Enzymatic fuel cells (EFCs) are emerging as promising technologies in renewable energy and biomedical applications, utilizing enzyme catalysts to convert the chemical energy of renewable biomass into electrical energy, known for their high energy conversion efficiency and excellent biocompatibility. Currently, EFCs face challenges of poor stability and catalytic efficiency at the cathodes, necessitating solutions to enhance the oriented immobilization of multicopper oxidases for improved heterogeneous electron transfer efficiency. This study successfully identified a surface-binding peptide (SBP, 13 amino acids) derived from a methionine-rich fragment (MetRich, 53 amino acids) in CueO through semirational design.

A Multifunctional DNA Nanoassembly for Cancer Cell Detection and Combined Gene-Chemotherapy Therapy.

Although DNAzyme is a promising gene therapy agent, low cellular uptake efficiency, poor biological stability, and the unsatisfactory effect of monotherapy limit its development. Herein, a multifunctional DNA nanoassembly (RCA product-aptamer-DNAzyme, RAD) was constructed for cancer cell detection and targeted delivery of doxorubicin (DOX) and DNAzyme. Briefly, the rolling circle amplification (RCA) product was employed as a scaffold, and each repeated sequence was designed to combine with three single-stranded DNA (ssDNA), which carried the aptamer AS1411 sequence, fluorescent group, and DNAzyme sequence, respectively.

High-Density Fermentation of P6: Enhancing Cell Viability via Sodium Alginate Enrichment.

exhibits a wide range of beneficial physiological functions, including maintaining intestinal microbiota balance, reducing serum cholesterol, and promoting digestive health. According to the specific nutrient requirements of P6, we investigated the effects of various carbon sources, nitrogen sources, trace elements, growth-promoting substances, as well as the initial pH and inoculum size on the growth of P6 under fermentation conditions. The optimal growth conditions for P6 were identified to facilitate high-density fermentation in small-scale fermenter production, achieving a cell concentration of 1.

Multifunctional Ru(III)/FeO/DNA nanoplatform for photothermal-enhanced photodynamic and chemodynamic cancer therapy.

Among the many cancer treatment methods, there have been many reports on the use of nanoplatforms with single treatment methods such as photothermal, photodynamic or chemodynamic for cancer treatment. In this study, Ru(III) with photodynamic effect and FeO nanoparticles with photothermal and chemodynamic effects are connected through long DNA chains with efficient active targeting rolling circle amplification to construct Ru(III)/FeO/DNA nano-platform realizes the combination of photothermal, photodynamic and chemodynamic treatment, which significantly improves the therapeutic effect of the nano-platform. Its multiple active targeting capabilities reduce the damage to normal cells.

[Clinical Study of Ibrutinib Combined with Venetoclax Regimen in the Treatment of Relapsed/Refractory Diffuse Large B-Cell Lymphoma].

Objective: To investigate the clinical efficacy of ibrutinib combined with venetoclax in the treatment of relapsed/refractory diffuse large B-cell lymphoma (R/R DLBCL), and to analyze the factors affecting efficacy and prognosis.

Methods: Clinical data of 62 R/R DLBCL patients admitted to our hospital from August 2017 to July 2022 were retrospectively analyzed. All patients were treated with ibrutinib combined with venetoclax.

Fibrillar adhesives with unprecedented adhesion strength, switchability and scalability.

Bio-inspired fibrillar adhesives have received worldwide attention but their potentials have been limited by a trade-off between adhesion strength and adhesion switchability, and a size scale effect that restricts the fibrils to micro/nanoscales. Here, we report a class of adhesive fibrils that achieve unprecedented adhesion strength (∼2 MPa), switchability (∼2000), and scalability (up to millimeter-scale at the single fibril level), by leveraging the rubber-to-glass (R2G) transition in shape memory polymers (SMPs). Moreover, R2G SMP fibrillar adhesive arrays exhibit a switchability of >1000 (with the aid of controlled buckling) and an adhesion efficiency of 57.

Cu-MoO-based nanozyme with enhanced peroxidase like activity for quinolone antibiotics detection.

The abuse of antibiotics has seriously threatened human health and living environment. Nevertheless, the detection of quinolones is currently mainly performed by high-cost and cumbersome means, such as High-Performance Liquid Chromatography (HPLC). Herein, we reported a novel method based on copper-doped MoO nanoparticles (Cu-MoO NPs) with peroxidase-like enhancement activity for the easy preparation, sensitive and rapid visualization of quinolone detection.

Periorbital purpura can be the only initial symptom of primary light chain amyloidosis: A case report.

Background: Primary light chain amyloidosis is a rare and complex disease with complex clinical features and is highly susceptible to misdiagnosis and underdiagnosis in the early stages.

Case Summary: We report a case of a 47-year-old female patient whose only initial symptom was periorbital purpura, which was not taken seriously enough. As the disease progressed, pleural effusion gradually appeared, and after systematic diagnosis and treatment, she was diagnosed with "primary light chain amyloidosis".

A Novel Score to Predict Individual Risk for Future Alzheimer's Disease: A Longitudinal Study of the ADNI Cohort.

Background: Identifying high-risk individuals with mild cognitive impairment (MCI) who are likely to progress to Alzheimer's disease (AD) is crucial for early intervention.

Objective: This study aimed to develop and validate a novel clinical score for personalized estimation of MCI-to-AD conversion.

Methods: The data from the Alzheimer's Disease Neuroimaging Initiative (ADNI) study were analyzed.

Breaking the Linear Scaling Relationship by Alloying Micro Sn to a Cu Surface toward CO Electrochemical Reduction.

The electrochemical CO reduction reaction (CORR) to HCOOH provides an avenue for reducing global accelerated CO emissions and producing high-value-added chemicals. Nevertheless, the presence of an inherent linear scaling relationship (LSR) between *OCHO and *HCOOH leads to the electrosynthesis of HCOOH being achieved at high cathodic potentials. In this work, by adjusting the different Cu:Sn ratio of SnCu alloys, we comprehensively explored the electrocatalytic 2e CORR performance toward the production of HCOOH.

Electrochemical Urea Production over Diatomic Fe-Ni Catalyst: a Mechanism for C-N Coupling.

Co-electrocatalytic reduction of CO and nitrate/nitrite as carbon and nitrogen sources to synthesize urea is an effective strategy to solve the energy problem and alleviate environmental pollution. In this work, combined density functional theory calculations with a constant-potential implicit solvent model, we proposed a strategy for the determination of the preferred reaction pathway and the potential window that is guided by the potential-dependent free energy change. It was found that on the FeNi-N-C surface, the C-N coupling occurs between *NHO and the protonated CO in the potential window of -2.

Rolling Circle Amplification-Based Self-Assembly to Form a "GPS-Nanoconveyor" for In Vitro Targeted Imaging and Enhanced Gene/Chemo (CRISPR/DOX) Synergistic Therapy.

The GPS-Nanoconveyor (MA-NV@DOX-Cas13a) is a targeted nanoplatform designed for the imaging and gene/chemotherapy synergistic treatment of melanoma. It utilizes rolling circle amplification (RCA) products as a scaffold to construct a DNA "Nanoconveyor" (NV), which incorporates a multivalent aptamer (MA) as a "GPS", encapsulates doxorubicin (DOX) in the transporter, and equips it with CRISPR/Cas13a ribonucleoproteins (Cas13a RNP). Carrying MA enhances the ability to recognize the overexpressed receptor nucleolin on B16 cells, enabling targeted imaging and precise delivery of MA-NV@DOX-Cas13a through receptor-mediated endocytosis.

Comprehensive transcriptomic and metabolomic insights into simultaneous CO sequestration and nitrate removal by the Chlorella vulgaris and Pseudomonas sp. consortium.

Simultaneous CO sequestration and nitrate removal can be achieved by co-cultivation of Chlorella vulgaris with Pseudomonas sp. However, a comprehensive understanding of the synergistic mechanism between C. vulgaris and Pseudomonas sp.

A Deep Learning Method for Bearing Cross-Domain Fault Diagnostics Based on the Standard Envelope Spectrum.

Intelligent fault diagnostics based on deep learning provides a favorable guarantee for the reliable operation of equipment, but a trained deep learning model generally has low prediction accuracy in cross-domain diagnostics. To solve this problem, a deep learning fault diagnosis method based on the reconstructed envelope spectrum is proposed to improve the ability of rolling bearing cross-domain fault diagnostics in this paper. First, based on the envelope spectrum morphology of rolling bearing failures, a standard envelope spectrum is constructed that reveals the unique characteristics of different bearing health states and eliminates the differences between domains due to different bearing speeds and bearing models.

Irisin inhibits microglial senescence via TFAM-mediated mitochondrial metabolism in a mouse model of tauopathy.

Background: The accumulation of senescent microglia has been highlighted as a critical contributor to the progression of tauopathies. Irisin, a muscle-derived hormone produced by the proteolytic cleavage of Fibronectin-domain III containing 5 (FNDC5), mediates the pleiotropic effects of exercise on the physical body. Herein, we investigate the potential role of irisin in microglial senescence in tauopathies.

The extracellular polymeric substances (EPS) accumulation of Spirulina platensis responding to Cadmium (Cd) exposure.

Spirulina platensis can secrete extracellular polymeric substances (EPS) helping to protect damage from stress environment, such as cadmium (Cd) exposure. However, the responding mechanism of S. platensis and the secreted EPS to exposure of Cd is still unclear.

Risk factors of electrical status epilepticus during sleep in children with benign childhood epilepsy with centro-temporal spikes.

Objective: To explore risk factors of electrical status epilepticus during sleep in children with benign childhood epilepsy with centro-temporal spikes (BECT).

Methods: This is a clinical comparative study. The subjects of study were 67 children with BECT from the Outpatient Department of Pediatric Neurology in Xingtai People's Hospital from January 2019 to January 2022.