Avermectin induced vascular damage in zebrafish larvae: association with mitochondria-mediated apoptosis and VEGF/Notch signaling pathway.

Avermectin is a highly effective insecticide that has been widely used in agriculture since the 1990s. In recent years, the safety of avermectin for non-target organisms has received much attention. The vasculature is important organs in the body and participate in the composition of other organs.

Mechanisms Underlying the Overlooked Chiral Fungicide-Driven Enantioselective Proliferation of Antibiotic Resistance in Earthworm Intestinal Microbiome.

From a "One Health" perspective, the global threat of antibiotic resistance genes (ARGs) is associated with modern agriculture practices including agrochemicals application. Chiral fungicides account for a considerable proportion of wildly used agrochemicals; however, whether and how their enantiomers lead to differential proliferation of antibiotic resistance in agricultural environments remain overlooked. Focused on the soil-earthworm ecosystem, we for the first time deciphered the mechanisms underlying the enantioselective proliferation of antibiotic resistance driven by the enantiomers of a typical chiral fungicide mandipropamid (i.

Simultaneous Detection of Multiple Plant Growth Regulator Residues in Cabbage and Grape Using an Optimal QuEChERS Sample Preparation and UHPLC-MS/MS Method.

Background: At present, plant growth regulators (PGRs) are widely used in agricultural and forestry production. PGRs, like traditional pesticides, have certain toxicities. Naively excessively applying them will cause the acute and chronic poisoning of humans and animals and potentially harm human health.

The optimization and establishment of QuEChERS-UPLC-MS/MS method for simultaneously detecting various kinds of pesticides residues in fruits and vegetables.

Objectives: The quality safety supervision and test of agricultural products urgently need a very excellent analytical method with simultaneously detecting many components in order to assess, prevent and control pesticide residues.

Methods: In this research, three fruits and three vegetables produced in Shanghai were selected as the materials, 54 pesticide residues were detected with the ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method on the basis of optimized QuEChERS method according to different materials properties.

Results: The results showed that: all samples were directly extracted by acetonitrile containing 1% (v/v) acetic acid; complex matrix samples were purified by a mixed sorbent of 300mg MgSO+100mg PSA+100mg C18+0.

[Ectogenous fragile histidine triad gene inhibits the malignant phenotype of human lung cancer cell line A549 in vitro and in vivo].

Objective: To investigate the inhibition effects of fragile histidine triad (FHIT) gene on the malignant growth of A549 cell line.

Methods: A mammalian expression vector PEGFP-FHIT was constructed and transfected into the A549 cell line by lipofectamine. Then the transfected cell line was screened by G418.

[The relationship between genetic polymorphism of metabolizing enzymes and the genetic susceptibility to lung cancer].

Objective: To investigate the relationship between the gene polymorphism of metabolizing enzymes and the genetic susceptibility to lung cancer as well as to study the synergistic effects between smoking and the genes.

Methods: A case-control study (case = 217, control = 200) was carried out to compare the frequent distribution of CYP1A1, 2E1, 2D6 and GSTM1 genotypes between the lung cancer group and the control group with a polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) method and to analyze the relationship between these genes and smoking.

Results: GSTM1-null genotype frequency was 58.

[Inhibitory effect of p53 with deletion of c-terminal 356 - 393 amino acids on malignant phenotype of human lung cancer cell line].

Objective: To study the effect of extraneous p53 gene with deletion of c-terminal 356 - 393 amino acids on inhibition of malignant phenotype of human lung cancer cell line.

Methods: Recombinant plasmid pEGFP-p53 (del) with codon deletion of c-terminal 37 amino acids from 393 to 356 region and pEGFP-p53 (wild type) were constructed. The human lung cancer cell line 801D served as a receipt cell had p53 deletion and mutation at 248 codon.