[Effects of hypoxia on the expression of heparanase and invasiveness of Hep-2 laryngeal carcinoma cell].

Objective: To evaluate the effects of hypoxia on the expression of heparanase and the invasiveness of Hep-2 laryngeal carcinoma cell line.

Methods: Hep-2 cells were incubated at either normoxia (37 degrees C, 5% CO2, 20% O2) or hypoxia (37 degrees C, 5% CO2, 2% O2) condition for 6 h,12 h,24 h,36 h. Flow cytometry was used to detect the protein expression of heparanase under different hypoxia conditions.