Novel Copper Complexes That Inhibit the Proteasome and Trigger Apoptosis in Triple-Negative Breast Cancer Cells.

Five innovative ternary copper(II) complexes [Cu(OH-PIP)(Phe)Cl](), [Cu(OH-PIP)(Gly)(HO)]NO·2HO (), [Cu(OH-PIP)(Ala)(Cl)]·HO (), [Cu(OH-PIP)(Met)]PF·2HO (), and [Cu(OH-PIP)(Gln)(HO)](Cl)·3HO () have been synthesized and characterized by infrared spectroscopy, elemental analysis, and single crystal X-ray diffraction analysis. X-ray crystallography indicates that all Cu atoms are five-coordinated in a square-pyramidal configuration. The complexes have been screened for cytotoxicity against human breast cancer cell lines MCF-7, MDA-MB-231, and CAL-51.

[Effects of nitrogen form on growth and quality of Chrysanthemums morifolium].

This paper is aimed to study the effects of nitrogen form on the growth and quality of Chrysanthemums morifolium at the same nitrogen level. In order to provide references for nutrition regulation of Ch. morifolium in field production, pot experiments were carried out in the greenhouse at experimental station of Nanjing Agricultural University.

[Effects of low-level laser irradiation on rat mesenteric microcirculatory disturbance during early stage of endotoxemia].

Objective: To observe the effects of low-level laser irradiation on mesenteric microcirculation of rats in vivo in the early stage of endotoxemia (ETM).

Methods: The experimental model of ETM was reproduced by injection of lipopolysaccharide (LPS). Sixty healthy male Sprague-Dawley (SD) rats were divided into three groups used random number table: control group, LPS group and low-level laser irradiation group, each group included 20 rats which were subdivided into four temporal subgroups (1, 2, 4, 6 hours, respectively).

A clinically relevant orthotopic xenograft model of ependymoma that maintains the genomic signature of the primary tumor and preserves cancer stem cells in vivo.

Limited availability of in vitro and in vivo model systems has hampered efforts to understand tumor biology and test novel therapies for ependymoma, the third most common malignant brain tumor that occurs in children. To develop clinically relevant animal models of ependymoma, we directly injected a fresh surgical specimen from a 9-year-old patient into the right cerebrum of RAG2/severe complex immune deficiency (SCID) mice. All five mice receiving the initial transplantation of the patient tumor developed intracerebral xenografts, which have since been serially subtransplanted in vivo in mouse brains for 4 generations and can be cryopreserved for long-term maintenance of tumorigenicity.

N-(3,4-Diethoxy-phen-yl)acetamide.

In the title compound, C(12)H(17)NO(3), the conformations of the N-H and C=O bonds are anti to each other. In the crystal structure, N-H⋯O hydrogen-bond inter-actions help to establish the packing.

[Effects of anisodamine on microcirculation of the asystole rats during the cardiopulmonary resuscitation].

Objective: To study the effects of anisodamine (Ani) on microcirculation and reperfusion volume of intestine wall in Wistar rats during cardiopulmonary resuscitation (CPR) for cardiac arrest (CA).

Methods: Healthy Wistar rats subjected to CA and resuscitation were randomly divided into four groups, 15 rats in each group. After a 4-minute-non-intervention interval, CPR was started.

[Perlecan participates in the protection of ischemic myocardium induced by basic fibroblast growth factor: experiment with rats].

Objective: To investigate if perlecan (PN) is involved in the myocardial protection of basic fibroblast growth factor (bFGF) in acute myocardial infarction.

Methods: Twenty-four Wistar rats were randomized into 3 groups: myocardial infarction group (MI group, n = 8, undergoing ligation of the descending anterior branch of the left coronary artery), bFGF + MI group (n = 10, injected with bFGF into the border myocardium between the infracted and non- infracted areas immediately after the ligation of the descending anterior branch), and sham operation group (n = 6, undergoing sham operation and injection of normal saline). 24 h, 14 days, and 28 days after the operation the hemodynamic parameters, infarct size, and microvessel density (MVD) were observed.

[Histopathology analysis of microemboli after thrombolysis].

Objective: To observe the changes in fibrin of microemboli in micro-embolism soon after thrombolysis, in order to provide a theoretical basis for micro-thrombolytic therapy.

Methods: Thirty adult male SD rats weighing (180+/-10)g were randomized into five groups: 5, 30, 60 and 90 minutes groups after micro-thromboembolization and the control group. Rat cremasteric artery was embolized with photochemical method, and micro-thrombi were produced after thrombolysis by staphylokinase.

[The changes in effective local blood perfusion and compensatory ventilation in different lung areas of acute respiratory distress syndrome rabbits model].

Objective: To discuss the mechanism of severe hypoxemia that induced with acute respiratory distress syndrome (ARDS) by way of observing the effective local blood perfusion in different lung areas of ARDS rabbits.

Methods: ARDS rabbit models were established by injecting oleic acid through central vein, and the results of effective local blood perfusion in different areas of rabbit lung (upper area, abdominal area, and dorsal area of right lung)with PIM-II laser perfusion imager and arterial blood gas under different ventilation modes [large tidal volume ventilation, low tidal volume ventilation + positive end-expiratory pressure (PEEP), prone position + large tidal volume ventilation, and prone position + low tidal volume ventilation + PEEP] were measured.

Results: The results of oxygenation index were significantly getting worsen after lung injury, and after receiving lung protective ventilation (including low tidal volume + PEEP, prone position + low tidal volume + PEEP), the indexes were taking a turn to the better.