Early autoimmunity and outcome in virus encephalitis: a retrospective study based on tissue-based assay.

Unlabelled: To explore the autoimmune response and outcome in the central nervous system (CNS) at the onset of viral infection and correlation between autoantibodies and viruses.

Methods: A retrospective observational study was conducted in 121 patients (2016-2021) with a CNS viral infection confirmed via cerebrospinal fluid (CSF) next-generation sequencing (cohort A). Their clinical information was analysed and CSF samples were screened for autoantibodies against monkey cerebellum by tissue-based assay.

[Effects of Human Immunodeficiency Virus-positive Mothers Receiving Antiretroviral Therapy to Prevent Mother-to-child Transmission on the Growth and Development of 18-month-old Children in Lingshan County of Guangxi].

Objective To evaluate the effects of antiretroviral therapy(ART)for the prevention of mother-to-child transmission(PMTCT)of acquired immune deficiency syndrome(AIDS)on the growth and development of 18-month-old children born by human immunodeficiency virus(HIV)-positive pregnant women in Lingshan County,Guangxi Zhuang Autonomous Region,and provide scientific evidence for improving the ART medication plan for PMTCT.Methods Lingshan County,ranking the first in the HIV-epidemic counties of Guangxi,was selected as the research site.According to the design of retrospective case-control study,we assigned all the subjects into the case group and the control group:(1)The case group included the HIV-positive pregnant women who had received ART for PMTCT and their HIV-negative infants in Lingshan County from 2010 to 2017.

Deviation influences on sectional image reconstruction in optical scanning holography using a random-phase pupil.

In this paper, we analyze the influence of two kinds of deviation errors on sectional image reconstruction for an optical scanning holography system using a random-phase pupil. The first deviation occurs in the lateral pixel position while the second occurs in the pixel value of the decoding function. Theoretical analysis and numerical simulation show that these two deviations may lead to noise in the reconstructed image.

The impact of expressions of CD97 and its ligand CD55 at the invasion front on prognosis of rectal adenocarcinoma.

Background: Various evidence show that CD97 plays an important role in tumor differentiation, migration, invasiveness, and metastasis by binding its cellular ligand CD55. CD55 is a complement regulatory protein expressed by cells to protect them from bystander attack by complement, and it has been shown to be an indicator of poor prognostic in several cancers.

Methods: CD97 and CD55 stains were detected in tumor tissues from 71 cases of rectal adenocarcinomas and their corresponding normal colorectal tissues by immunohistochemistry.

Prognostic and metastatic value of phosphatase of regenerating liver-3 in invasive breast cancer.

Purpose: The aim of this study was to investigate the expression of the PRL-3 in human invasive breast cancer and to evaluate its clinical and prognostic significance. Its potential role in the invasive-metastatic properties of invasive breast cancer was also investigated.

Methods: Protein expression of PRL-3 was evaluated by immunohistochemistry for a consecutive series of 82 invasive human breast cancer tissues and 63 matched lymph node metastases, including PRL-3 mRNA expression analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) in malignant, nonmalignant breast tissue samples and lymph node metastases.

[Bronchoscopic and CT findings of invasive tracheobronchial and pulmonary aspergillosis in patients without immunodeficiency].

Objective: To explore the bronchoscopic and CT findings of invasive tracheobronchial and pulmonary aspergillosis in patients without immunodeficiency.

Methods: Clinical data and bronchoscopic and CT findings of 6 patients with tracheobronchial and pulmonary aspergillosis were reviewed from January 2004 to August 2008.

Results: All the patients had no immunodeficiency diseases.

[Effect of tadalafil therapy upon penile cavernous tissues in spontaneously hypertensive rats].

Unlabelled: OBJECTIVE; To observe the plasma concentration change of endothelin-1 (ET-1) and the histomorphological changes in penile cavernous body after administrating a continuous low dose of tadalafil into spontaneously hypertensive rats (SHR).

Methods: Forty SHR rats were randomly divided into four groups of blank group, placebo group, continuous low-dose group and intermittent dose group with 10 rats in each. The animals were administrated with different doses of tadalafil via an intragastric route.

[Relationship of neuroendocrine differentiation to biological behavior of prostate cancer].

Objective: To investigate the relationship between neuroendocrine differentiation (NED) in prostate cancer and hormone refractory prostate cancer.

Methods: Fifty-five prostate cancer specimens were obtained from 55 patients following intermittent androgen blockade during operation of transurethral resection of prostate. Monoclonal antibody immunohistochemistry was used to detect the expression of chromogranin A (CgA), a specific marker of neuroendocrine cell in the specimens.

FOXP1 expression predicts polymorphic histology and poor prognosis in gastric mucosa-associated lymphoid tissue lymphomas.

Unlabelled: RATIONALE/HYPOTHESIS: Forkhead box protein P1 (FOXP1) is one member of the forkhead box protein P (FOXP) subfamily, which are transcription factors that mediate signaling and affect gene regulation, and elevated expression of FOXP1 has been reported to correlate with poor prognosis of diffuse large B-cell lymphoma (DLBCL). Recently, it was also found that FOXP1 expression occurs in mucosa-associated lymphoid tissue (MALT) lymphomas.

Objective: FOXP1 expression and its relationship to morphology and prognosis in a series of 43 gastric MALT lymphomas were investigated retrospectively.

Enhanced cellular uptake of chlorine e6 mediated by stearic acid-grafted chitosan oligosaccharide micelles.

Chlorines are attractive compounds for photodynamic therapy because of their high absorption in the red wavelength region. The stearic acid-grafted chitosan oligosaccharide (CSO-SA) micelles have been presented as potential candidates for intracellular drug delivery carrier because of their special structure. In this study, CSO-SA micelles were prepared to encapsulate chlorine e6 (Ce6).

[Mechanism of liver injury in severe acute pancreatitis rats and role of melatonin].

Objective: To investigate the activation level of nuclear factor-kappaB (NF-kappaB) in liver injury caused by severe acute pancreatitis (SAP) and the protective role of melatonin against liver injury.

Methods: Ninety-six Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: SAP group undergoing injection of sodium taurocholate to establish SAP models, melatonin (Mel) treatment group undergoing intraperitoneal injection 50 mg/kg 30 minutes before the establishment of SAP models, and sham operation group (Sham group). 4, 12, 24, and 48 hours after the onset of operation, blood samples were collected from the inferior vena casa of 8 rats from each group to measure the serum level of amylase (AMY) and alanine transaminase (ALT) by iodine colorimetry, and to detect the serum level of tumor necrosis factor-alpha (TNF-alpha) by ELISA.

[Effects of acupuncture on rehabilitation of nervous functions in the stroke patient of different OCSP types].

Objective: To probe into effects of acupuncture on rehabilitation of nervous functions in the stroke patient of different OCSP types.

Methods: The stroke patients were randomly divided into 4 subtypes (LACI, TACI, PACI, POCI) based on the OCSP clinical classification. The patients of every subtype were randomly sub-divided into an observation group and a control group.

[Frequency and transcript variant analysis of gene fusions between TMPRSS2 and ETS transcription factor genes in prostate cancer].

Objective: To investigate the frequencies and types of fusions between the transmembrane protease serine 2 (TMPRSS2), ETS-related gene (ERG), ETS variant-1 (ETV1), and ETS variant-4 (ETV4) genes in prostate cancer (PCa) and significance thereof.

Methods: Biopsy samples of prostate were obtained under transrectal ultrasound (TRUS) from 32 PCa patients, aged (74 +/- 8) and 34 patients with benign prostate hyperplasia (BPH). Nested RT-PCR and direct DNA sequencing were used to detect the fusion genes of TMPRSS2/ERG, TMPRSS2/ETV1, and TMPRSS2/ETV4.

[Protein expression and clinical significance of cyclooxygenase 2 and nuclear factor kappa B in gastric mucosa-associated lymphoid tissue lymphoma].

Objective: To investigate the protein expression of cyclooxygenase 2 (COX-2) and nuclear factor kappa B (NF-kappaB) in gastric mucosa-associated lymphoid tissue (MALT) lymphoma and its clinical significance.

Methods: Protein expression of COX-2 and NF-kappaB in gastric MALT lymphoma were examined by immunohistochemistry of Envision two-step method. The correlations of COX-2 and NF-kappaB expression with Helicobacter pylori (Hp) infection, clinical stage, depth of tumor invasion, tumor size, recurrent rate and treatment were analyzed by univariate, multivariate and Pearson analysis.

Cellular uptake and cytotoxicity of shell crosslinked stearic acid-grafted chitosan oligosaccharide micelles encapsulating doxorubicin.

Stearic acid-grafted chitosan oligosaccharide (CSO-SA) with 3.48% amino-substituted degree (SD%) was synthesized by coupling reaction. The CSO-SA could self-aggregate to form micelle with a critical micelle concentration (CMC) at 0.

[Quantitative detection of differential display code 3 mRNA in peripheral blood of patients with prostate cancer].

Objective: To investigate the expression of DD3 mRNA in the peripheral blood and its value in diagnostic of prostate cancer (Pca).

Methods: Thirty-five untreated Pca patients, aged 72 (53 - 83), 58 Pca patients treated with endocrinotherapy, aged 74 (53 - 86), and 59 patients with benign prostatic hyperplasia, aged 71 (48 - 85), underwent peripheral blood sample collection one week before or after digital examination. RT-PCR was used to examine the level of DD3 mRNA.

[Change of eustachian tube surfactant of guinea pigs suffering from secretory otitis media].

Objective: To observe the change of surface active agents of eustachian tube of guinea pigs suffering from secretory otitis media, to explore the effects of surface active agents on secretory otitis media.

Methods: To establish the animal model of guinea pigs suffering from secretory otitis media. To analyse the changes of biochemical component and activity of eustachian tube's surfactant, meanwhile; to observe surfactant change after ectogenic surfactant treatment.

[Endothelin-1 expression and quantitative analysis in astrocytomas].

Background & Objective: It is demonstrated that endothelin-1 (ET-1) is synthesized by and released from certain malignant tumor cells, and it plays an important role in growth and development of tumor. This study was designed to investigate the expression of ET-1 in astrocytomas and the relationship between the ET-1 quantity and the grades of astrocytomas.

Method: ET-1 expression was determined in 70 astrocytoma specimens using Streptavidin-Peroxidase method and image analysis technology.