[Expression of the CREM gene in the semen and testis of miR-199b-5p knockout male mice].

Objective: To investigate the effect of miR-199b-5p knockout on the expression of the CREM gene in the semen and testis of mice.

Methods: We selected 8 miR-199b-5p knockout (KO) male mice (the KO group) and another 8 wild-type (WT) C57BL/6 male mice (the WT group), and observed the changes in their body weight, testicular index, testis structure and sperm morphology. We detected sperm concentration under the microscope, determined the mRNA and protein expression levels of CREM in the semen and testis by RT-qPCR and Western blot respectively, and predicted the targeting relationship between CREM and miR-199b-5p using the online database.