Interactions between the gut micro-community and transcriptome of Culex pipiens pallens under low-temperature stress.

Background: Culex pipiens pallens (Diptera: Culicidae) can survive at low temperature for long periods. Understanding the effects of low-temperature stress on the gut microflora and gene expression levels in Cx. pipiens pallens, as well as their correlation, will contribute to the study of the overwintering mechanism of Cx.

[Study on the mechanisms underlying overwintering of using iTRAQ-based quantitative proteomic analysis].

Objective: To compare the difference of protein expression between the post-overwintering stage and the diapauses preparation stage in , so as to reveal the mechanisms underlying the overwintering diapause of .

Methods: A quantitative proteomic analysis was performed in before and after overwintering diapause by using isobaric tags for relative and absolute quantification (iTRAQ) labeling.

Results: A total of 244 differentially expressed proteins were identified in before and after overwintering diapause, including 126 up-regulated proteins and 118 down-regulated proteins.

[Seasonal fluctuations of mosquitoes and application of two surveillance tools in Taibai Lake area of Jining City].

Objective: To investigate the seasonal fluctuations of mosquitoes in Taibai Lake area of Jining City, and evaluate the application effects of two mosquito trapping tools (Gongfu Xiaoshuai mosquito lamp and WJ-C mosquito sampler), so as to provide references for the effective surveillance, prevention and control of local mosquito vectors.

Methods: The overnight capture of mosquitoes with a man-bait net trap, Gongfu Xiaoshuai mosquito lamp, and WJ-C mosquito sampler was performed to collect mosquitoes. The species of adult mosquitoes and seasonal fluctuations were investigated and analyzed.

[Analysis of population genetic diversity of mosquitoes from Shandong Province based on mitochondrial DNA cytochrome oxidase subunit I gene fragment].

Objective: To explore the characteristics of gene sequence of mtDNA-COⅠ of from different geographical regions in Shandong Province and different resistant strains from the lab and five common mosquito species, and analyze the genetic diversity of these mosquitoes.

Methods: Adult mosquitoes were collected from Jinan, Jining, Qingdao cities and other places in Shandong Province. The sensitive, dichlorvos-resistant, pyrethroid-resistant and propoxur-resistant strains were reared in the lab.

Epidemiological analysis of 133 malaria cases in Shanxian county, Shandong Province, China.

Objective: To conduct an analysis of the epidemiological changes in malaria that have occurred in Shanxian county from 2002 to 2016.

Methods: A retrospective study was conducted and data were collected from web-based reporting system to explore the epidemiological characteristics in Shanxian county from 2002 to 2016. All individual case information was obtained from village malaria servicers organized by the local Shandong Institute of Parasitic Diseases.

[Research Progress on the Molecular Mechanisms of Mosquito Innate Immunity].

As the important vector of the mosquito-borne diseases, mosquitos rely on their innate immune system against pathogens infection, which includes cellular immunity and humoral immunity. Both of them are interacted and coordinated, via the pattern recognition receptors, immune signaling, antimicrobial peptides, phenoloxidase-based melanization, phagocytosis, and so on. This paper reviews the research progress on the molecular mechanisms of mosquito innate immunity.

[Experimental observation on host preference of Anopheles sinensis].

Objective: To observe the host preference of Anopheles sinensis captured by outdoor human or cattle baits.

Methods: A large number of non-blood-fed An. sinensis females were collected by overnight trapping outdoor with human and cattle in the rice paddy field in Shan County of Shandong Province, and took back to the lab, and individually labeled as human baits group and cattle baits group, fed with mouse blood.

[Sequence analysis of ribosomal DNA internal transcribed spacer 2 of sympatric populations of Anopheles sinensis of different feeding preferences].

Objective: To investigate the existence of genetic divergence of sympatric populations of Anopheles sinensis of different feeding preferences based on the rDNA-ITS2 sequence differences.

Methods: A large number of wild anopheles populations were trapped all night by man-baited net and calf-baited net that had been set up between high-density natural villages of An. sinensis populations and vector-breeding sites, from which two groups of An.

The dynamic changes of plasma neuropeptide y and neurotensin and their role in regulating cerebral hemodynamics in neonatal hypoxic-ischemic encephalopathy.

Hypoxic-ischemic encephalopathy (HIE) is a common cause of neonatal encephalopathy and is one of the most important causes of neonatal death and disabilities, especially those infants with moderate to severe encephalopathy. However, the pathogenesis of HIE still remains unclear. The purpose of this study was to explore the dynamic changes in plasma neuropeptide Y (NPY) and neurotensin (NT) as well as their role in regulating cerebral hemodynamics in HIE patients.