[Estimation of the flux of inorganic nitrogen flowing into the East China Sea].

The flux of inorganic nitrogen flowing into the East China Sea was estimated based on the systematic analysis of all the pollution sources from 1980-2005. The result showed that the flux of inorganic nitrogen had been increasing from the early 1980s to the early 21st century. In detail, the flux was about 8.

[Fluorescence discrimination technique for phytoplankton based on the wavelet analysis].

Daubechies7 (db7) wavelet was selected to decompose the 3-D fluorescence spectra of 27 species of phytoplankton belonging to 22 generas of 6 divisions found in major lakes, then the scale vectors and time-series vectors were obtained as candidates for feature spectra. The third scale vector (Ca3) of db7 was chosen as feature spectra by Bayesian discriminant analysis, and the reference spectra were obtained via hierarchical cluster analysis to feature spectra. Based on the above data, a fluorescence discrimination technique was developed by multiple linear regression resolved by non-negative least squares.

[Studies on micelle behaviors of sophorolipid biosurfactant by steady-state fluorescence probe method].

The critical micelle concentration (CMC) of sophorolipid was determined by steady-state fluorescence probe method in which pyrene was used as fluorescence probe. Meanwhile, the changes in the sophorolipid CMC were examined in the addition of NaCl and aliphatic alcohol, respectively. The results showed that the CMC of sophorolipid was 1.

[Toxic effects of chloride tributyltin on the predominant phytoplankton species of China coastal sea].

Toxic effects of tributyltin (TBT) on the growth, peak diameter and photosynthetic activity (F(v)/F(m)) of fifteen predominant phytoplankton species in China coastal sea of Leptocylindru danicus, Skeletonema marinoi, Asterionella japonica, Bacilaria paxillifera, Chaetoceros debilis, Chaetoceros socialis, Thalassiosira, Amphidinium carterae, Karenia mikimotoi, Prorocentrum minimum, Gymnodinium sp., Scrippsiella trochoidea, Gymnodinium simplex, Prorocentrum triestinum and Heterosigma akashiwo were systematically studied. 96 h-EC50 of TBT were 136.

Significant fluorescence enhancement by supramolecular complex formation between berberine chloride and cucurbit(n=7)uril and its analytical application.

The supramolecular interaction of cucurbit(n=7)uril (Q[7]) with berberine chloride (BER) has been studied in aqueous solution at pH 2.0 and room temperature by spectro-fluorimetry. The association constant of the complex was 2.

[Research on the 3D fluorescence spectra differentiation of phytoplankton by coiflet2 wavelet].

In the present paper, the authors utilize the wavelet base function coiflet2 (coif2) to analyze the 3D fluorescence spectra of 37 phytoplankton species belonging to 30 genera of 7 divisions, and these phytoplankton species include common species frequently causing harmful algal blooms and most predominant algal species in the inshore area of China Sea. After the Rayleigh and Raman scattering peaks were removed by the Delaunay triangulation interpolation, the fluorescence spectra of those phytoplankton species were transformed with the coiflet2 wavelet, and the scale vectors and the wavelet vectors were candidate for the feature spectra. Based on the testing results by Bayesian analysis, the 3rd scale vectors were the best feature segments at the division level and picked out as the fluorescence division feature spectra of those phytoplankton species, and the group of the 3rd scale vectors, the 2nd and 3rd wavelet vectors were the best feature segments at the genus level and chosen as the fluorescent genus feature spectra of those phytoplankton species.

[Fluorescence characterization of dissolved organic matter in East China sea].

Sea water samples were collected in the East China Sea in March and April, 2005, and three-dimensional fluorescence of dissolved organic matter was measured by fluorescence excitation-emission matrix spectroscopy. The position, number and intensity of fluorescence peak in the spectra and the relations of the peaks were analyzed to determine the type, distribution and origin of the fluorescence dissolved organic matter. Seven types of fluorescence peaks were detected from the samples.

[Role of irradiance on the seasonality of Skeletonema costatum Cleve blooms in the coastal area in East China Sea].

With field culture experiments and model calculations, the role of light on why in both spring and summer Skeletonema costatum Cleve blooms can occur in the coastal area in East China Sea were studied. The results show that the growth optimal light intensity (I(opt)) of S. costatum increases gradually to a maximum of 121.

[Effects of irradiance on blooms of the dinoflagellate Prorocentrum donghaiense Lu in the coastal area in East China Sea].

With field culture experiments and model calculations, the natural-light-dependent growth and the optimal light layers in sea water for growth of red tide dinoflagellate Prorocentrum donghaiense Lu were studied in order to analyze the role of light on algal blooms in the coastal area in East China Sea in spring. The results show that the relationship of growth and light can be well described by Steele's equation, and the optimal light intensity (Iopt) of P. donghaiense is (38.

[Seasonal variation and spatial distribution of nutrients and their relationships with harmful algal blooms in coastal area of the East China Sea].

Based on the data from four cruises that carried out in the Changjiang River estuary and its adjacent areas from 2002-04 to 2003-02, the seasonal variation and spatial distribution of nutrients were analyzed, and the relationship between nutrients condition and the harmful algal blooms (HABs) was also discussed. Results showed that the annual average concentration of nutrient was (17.93 +/- 2.

[Study on the characters of phytoplankton chlorophyll fluorescence excitation spectra based on fourth-derivative].

Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra.

[Excitation-emission-matrix spectral property of dissolved organic matter in seawater of Jiaozhou Bay, China].

Excitation-emission matrix spectroscopy (EEMs) was applied to measure the fluorescence properties of dissolved organic matter (DOM) in seawater collected from Jiaozhou Bay in June, 2005. The study areas include central Bay area, receptor areas of Dagu River, Licun River and Haibo River. The aim was to investigate the influence of discharges from several typical rivers to the receiving seawater.

Spatial distribution of dissolved Pb, Hg, Cd, Cu and As in the Bohai Sea.

Characteristics of the spatial distribution of selected dissolved heavy metals were analyzed during large scale surveys from August 12 to 25, 2003 in the Bohai Sea. Dissolved Pb was the only element with average concentrations higher than the grade-one sea water quality standard of China. The spatial distribution of dissolved Pb in surface water was similar to those of Cd, Cu and As, where the isopleths generally indicated decreasing values from the bays to the central areas.

[Discrimination of Red Tide algae by fluorescence spectra and principle component analysis].

Fluorescence discrimination technology for 11 species of the Red Tide algae at genus level was constructed by principle component analysis and non-negative least squares. Rayleigh and Raman scattering peaks of 3D fluorescence spectra were eliminated by Delaunay triangulation method. According to the results of Fisher linear discrimination, the first principle component score and the second component score of 3D fluorescence spectra were chosen as discriminant feature and the feature base was established.

[Exchange rates of dissolved nutrients at the sediment-water interface before and after diatom bloom in the East China Sea in spring].

Exchange rates of dissolved nutrients at the sediment-water interface were studied before and after diatom bloom from March to May in the East China Sea in 2005. Intact sediment cores which were sampled at 10 sites were incubated on board, and exchange rates were calculated in the function method. It was shown that sediment absorbed NO3(-) -N before bloom with the exchange rate ranging from - 1.

The application and comparison of several chemometric methods of excitation-emission matrix spectra in studying the interactions of metal complexes with DNA.

The interactions of fs DNA and two metal complexes [Cu(phen)SO4] x 2 H2O and [Ni(phen)SO4] x 2 H2O were explored by several chemometric methods, including parallel factor (PARAFAC), singular value decomposition-least squares (SVD-LS), and singular value decomposition-nonnegative least squares (SVD-NNLS) of excitation-emission matrix spectra (EEMs). The applications of SVD-LS and SVD-NNLS in this domain have been discussed. Rayleigh scatter part is avoided by ordered zero and reconstructed by linear interpolation.

[Research on characterization analysis of synchronous fluorescence spectra of living phytoplankton].

The synchronous fluorescence spectra of five phytoplankton species growing under three temperatures (25, 20 and 15 degrees C)and three illuminations (7000, 4100 and 1100 Lx)were measured and processed by multinomial smoothness and autoscaling to obtain the characteristic spectra. Principal component analysis was used to obtain standard spectra. The analysis shows that at different temperatures, the characteristic spectra of Skeletonema costatuma, Isochrysis galbana, and Platymonas helgolanidica show high similarities, while the spectra similarities of Alexandrium tamarense and Gymnodinium stein are not as good as the above three species.

[Synthesis and DNA binding spectroscopic studies of Cu(II)-HAsp-Im complexes].

Two novel complexes of [Cu(HAsp)ImH2O]SO4 x 4 H2O and [Cu(Asp)Im(OH)] x 4 H2O (HAsp = Aspartic acid molecule, Asp = Aspartic acid ion, Im = Imidazole) were synthesized and characterized by elemental analysis, IR spectroscopy and TG-DTA. The interactions of the complexes with sperm DNA were studied by electronic absorption and fluorescence spectra. Results showed that the reactions of the two complexes with DNA are obviously different: for [Cu(HAsp)ImH2O]SO4 x 4 H2O, it is intercalation companied by electrostatic effect, while [Cu(Asp)Im(OH)] x 4 H2O mainly cooperates with the nitrogen atom of the DNA base pair, which induces the breakage of DNA double helix.

[Ecological effect of No.0 diesel water accommodated fraction on marine algae].

With batch culture experiments in field and laboratory, the ecological effect of No. 0 diesel water accommodated fraction on marine algae was studied. A growth model of marine algae under grazing pressure and a model of growth effect on marine algae with different doses No.

Determination of multiclass pesticide residues in apple juice by gas chromatography-mass spectrometry with large-volume injection.

This study presents two GC-MS SIM methods, in combination with large-volume injection programmed-temperature vaporization (LVI-PTV) injection, for the determination of 141 pesticide residues in apple juice. The sample was extracted with ACN, and coextractives were removed with primary/secondary amine sorbent. ACN extract (20 microL) was injected into a PTV injection port in solvent vent mode, and the pesticides were determined by GC-MS using retention time locking software.

[Characteristics of absorption spectra of phytoplankton].

Nine typical phytoplankton species were chosen and cultivated under two temperatures (20 and 15 degrees C) and two illumi- nations (7000 and 1100 lux), and their absorption spectra at different growth period were measured. Firstly, singular value decomposition was used on the matrix, which is composed of the autoscaled spectra data. The S1/msigma(i=1)Si was used to compare the similarities of spectra.

[Selective algicidal activity of Gemini1231 biquaternary ammonium salt].

The growth-inhibiting effects of Gemini1231 surfactant on Prorocentrum donghaiense, Alexandrium tamarense, Gymnodinium sp., Heterosigma akashiwo, Skeletonema costatum , Platymonas helgolanidica and Platymonas subcordiforus were investigated. The results demonstrate that the growth of P.

[Effects of Cd (II) on the growth of 8 species of marine microalgae].

Effects of Cd (II) on the growth of 8 species of marine microalgae were studied in batch culture experiment. The experimental results show that higher doses of Cd (II) inhibite the growth of Heterosigma akashiwo Hada. Chaetoceros curvisetus.

Synthesis, crystal structure and DNA binding studies of a binuclear copper(II) complex with phenanthroline.

A new binuclear copper(II) complex, [Cu2Phen2Cl4] (Phen=1,10-phenanthroline), has been synthesized and characterized. Single crystal X-ray diffraction results suggest that this complex structure belongs to monoclinic crystal system, Cc (no. 9) with the cell dimensions: a=9.