[Cloning and Ion Transportation of Cryptosporidium andersoni ATP-binding Cassette 1 Gene].

Objective: To investigate the transportation of intracellular and extracellular K(+), Ca(2+), Na(+) and Mg(2+) under the function of Cryptosporidium andersoni ATP-binding cassette (CaABC) 1 gene.

Methods: CaABC1 gene was amplified by PCR using specifically designed primers. The eukaryotic expression plasmid pEGFP-C1-CaABC1 was constructed, and transfected into mouse intestinal epithelial cells via liposome transfection.

Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene.

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC.

[Isolation and identification of cow-origin Cryptosporidium isolates in Hefei].

Objective: To isolate cow-origin Cryptosporidium in Hefei, and identify its species.

Methods: 285 dairy cattle fecal samples collected from a farm in Hefei were examined by using floating saturated solution of sucrose and modified acid-fast staining. Cryptosporidium oocysts were isolated and purified from positive fecal samples.

[Research progress on nutrient transport and metabolism of Cryptosporidium].

Cryptosporidiosis is a worldwide zoonotic disease, and Cryptosporidium is coccidia-like parasite that develops in epithelial cells in digestive and respiratory tracts of human and animals. This review summarizes the specific function structure of Cryptosporidium, nutrient uptake, transport, metabolism, and the impact of Cryptosporidium on host nutrient absorption.