[HPLC Fingerprint Study and Quantitative Determination of Index Components of Pilea aquarum].

Objective: To establish an HPLC fingerprint and a quantitative determination method for determination of index components of Pilea aquarum.

Methods: The HPLC fingerprint of Pilea aquarum were determined on an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 µm), eluted with mobile phase containing of acetonitrile-0.

[Antitussive constituents of Disporum cantoniense].

The antitussive activity assay for the root extraction of Disporum cantoniense was carried out with coughing mice induced by ammonia liquor. The results showed that the ethanol and water extractions of D. cantoniense possess strong antitussive activity, and the high dose of the former was better than positive control, and then the constituents of the ethanol extraction were separated and purified by various modern chromatographic techniques.