[Study on electroporation of rare Actinomycete Micromonospora sp. 40027 with Streptomyces plasmid pSET152].

Electroporation of Micromonospora sp. 40027 isolated from soil was studied with Streptomyces plasmid pSET152, an integrative vector commonly used in Streptomyces genetic manipulation. Transformant was not obtained by electroporation with germinated spores of Micromonospora sp.

[Studies on the isolation and biological characterization of actinophages infecting Micromonospora sp. 40027].

Three actinophages, phiHAU7, phiHAU9 and phiHAU11, were isolated from soil using Micromonospora sp. 40027 as a indicator strain. Three phages showed very narrow host-ranges.

[Determination of nanchangmycin and meilingmycin by high performance liquid chromatography].

A high performance liquid chromatographic method had been established for the separation and determination of two antibiotics produced by Streptomyces nanchangensis, polyether nanchangmycin and 16-membered macrolide meilingmycin. The latter is composed of several components. The operating conditions were Waters XTerra RP18 column (3.

[Construction of a temperature inducible shuttle expression vector and its application in Streptomyces].

pHZ1080, an E. coli-Streptomyces shuttle expression vector was constructed in order to explore the utilization of lambda phage regulated expression elements in Streptomyces. A 2.