Application of Metabonomics in Substance Abuse Toxicology Research.

Abstrct: Metabonomics is a relative discipline that develops after genomics and proteomics, and it is an important component of systems biology. It uses high-throughput and high-sensitivity instruments to perform qualitative and quantitative analysis of all metabolic components in specific biological samples under limited conditions and combines with multivariate statistics to analyze and process the data to obtain information about physiological, pathological or toxicological changes in organisms. In recent years, because of the complicated mechanism of substance abuse and the continuous emergence of new psychoactive substances, metabonomics is increasingly used in substance abuse research.

Analysis of silent information regulator 1 (SIRT1) gene polymorphisms in antituberculosis- drug-induced hepatotoxicity in a prospective cohort study.

Objective: Antituberculosisdrug-induced hepatotoxicity (ATDH) is a common and sometimes serious side effect related to tuberculosis (TB) treatment. A number of risk factors and host genetics contribute to the development of ATDH. However, genetic factors of ATDH remain to be identified.

Phylogenetic analysis of newly isolated grass carp reovirus.

Grass carp reovirus (GCRV) is a causative agent of haemorrhagic disease in grass carp that drastically affects grass carp aquaculture. Here we report a novel GCRV isolate isolated from sick grass carp that induces obvious cytopathic effect in CIK cells and name it as GCRV096. A large number of GCRV 096 viral particles were found in the infected CIK cells by electron microscope.

Molecular cloning and prokaryotic expression of vp5 gene of grass carp reovirus strain GCRV096.

VP5 is an outer capsid protein of grass carp reovirus (GCRV). It is predicted to involve in helping GCRV enter the host cells. In this study, the full-length vp5 gene (accession number in GenBank: JN206664.

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts.

Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. In this study, the mcp gene of LCDV and the cyt b gene of the host fish were selected as molecular markers, and the phylogenetic relationships between LCDV and its host were analyzed. The 25 LCDV isolates examined in this study were attributed to seven LCDV genotypes: genotype I (LCDV-1), genotype II (LCDV-cn, etc.

[Molecular phylogenetic relationships of Drosophila immigrans species group based on H2a-H2b spacer and Cyt b sequences].

In order to investigate molecular phylogenetic relationships of Drosophila immigrans species group, nucleotide sequences of H2a-H2b spacer regions and partial nucleotide sequences of Cytochrome b gene for five specieses subgroup in Drosophila immigrans species group and D. hydei which was selected as outgroup were acquired. Phylogenetic trees were reconstructed using maximum parsimony, maximum likelihood, neighbor joining and Bayesian methods based on H2a-H2b and Cyt b DNA sequences.