Identifying resistance molecules in TiO nanoparticle-tolerant strains to facilitate the development of strategies for combating TiO nanoparticle pollution.

The severity of environmental pollution caused by TiO nanoparticles (nTiO) is increasing, highlighting the urgent need for the development of strategies to combat nTiO pollution. Insights into resistance molecules from nTiO-tolerant strains may facilitate such development. In this study, we utilized multi-omics, genetic manipulation, physiological and biochemical experiments to identify relevant resistance molecules in two strains (Physarum polycephalum Z259 and T83) tolerated to mixed-phase nTiO (MPnTiO).

Physarum polycephalum macroplasmodium exhibits countermeasures against TiO nanoparticle toxicity: A physiological, biochemical, transcriptional, and metabolic perspective.

Concerns about the environmental and human health implications of TiO nanoparticles (nTiO) are growing with their increased use in consumer and industrial products. Investigations of the underlying molecular mechanisms of nTiO tolerance in organisms will assist in countering nTiO toxicity. In this study, the countermeasures exhibited by the slime mold Physarum polycephalum macroplasmodium against nTiO toxicity were investigated from a physiological, transcriptional, and metabolic perspective.

Construction of a directional T vector for cloning PCR products and expression in Escherichia coli.

In order to clone PCR products and express them effectively in Escherichia coli, a directional cloning system was constructed by generating a T vector based on pQE-30Xa. The vector was prepared by inserting an XcmI cassette containing an endonuclease XcmI site, a kanamycin selective marker, a multiple-cloning-site (MCS) region and an opposite endonuclease XcmI site into the vector pQE-30Xa. The T vector pQE-T with single overhanging dT residues at both 3' ends was obtained by digesting with the restriction enzyme XcmI.