Integrated analysis of transcriptome and metabolome reveals the regulatory mechanism of largemouth bass (Micropterus salmoides) in response to Nocardia seriolae infection.

Nocardia seriolae is a severe bacterial pathogen that has seriously affected the development of aquaculture industry. Largemouth bass (Micropterus salmoides) is a commercially significant freshwater fish that suffers a variety of environmental threats, including bacterial pathogens. However, the immune responses and metabolic alterations of largemouth bass to N.

Optimizing surgical outcomes for elderly gallstone patients with a high body mass index using enhanced recovery after surgery protocol.

Background: Rehabilitation of elderly patients with a high body mass index (BMI) after cholecystectomy carries risks and requires the adoption of effective perioperative management strategies. The enhanced recovery after surgery (ERAS) protocol is a comprehensive treatment approach that facilitates early patient recovery and reduces postoperative complications.

Aim: To compare the effectiveness of traditional perioperative management methods with the ERAS protocol in elderly patients with gallbladder stones and a high BMI.

Impacts of artificial reef on community structure and functional traits of macrobenthos near Furong Island, Shandong, China.

To evaluate the ecological restoration effect of artificial reefs and explore the impacts of artificial reefs age on the structure and functional traits of the macrobenthos community, two artificial reef areas with different reef ages in the coastal waters of Furong Island, Laizhou Bay were investigated in May, August, and October 2019. A total of 120 macrobenthos species were identified in the three seasons, including 72 species in the control area, 74 species in the artificial reef with short age (1 year), and 80 species in the artificial reef with greater age (4-5 years). was the dominant species in all areas.

Thermally activated bipyridyl-based Mn-MOFs with Lewis acid-base bifunctional sites for highly efficient catalytic cycloaddition of CO with epoxides and Knoevenagel condensation reactions.

Metal-organic frameworks (MOFs) have become preferred heterogeneous catalytic materials for many reactions due to their advantages such as porosity and abundant active sites. Here, a 3D Mn-MOF-1 [Mn(DPP)(HO)]·6HO (DPP = 2,6-di(2,4-dicarboxyphenyl)-4-(pyridine-4-yl)pyridine) was successfully synthesized under solvothermal conditions. This Mn-MOF-1 possesses a 3D structure constructed by the combination of a 1D chain and the DPP ligand and features a micropore with a 1D drum-like shaped channel.

Clinical Efficacy of Glucosamine plus Sodium Hyaluronate for Osteoporosis Complicated by Knee Osteoarthritis and Its Influence on Joint Function and Bone Metabolic Markers.

Background: Osteoporosis (OP) associated with knee osteoarthritis (KOA) is common in older men and postmenopausal women, and it is important to find reliable and effective treatments for this disease to improve joint function and bone metabolism in this population.

Objective: To clarify the clinical efficacy of glucosamine (GlcN) plus sodium hyaluronate (SH) for OP complicated by KOA (OP + KOA) and its influence on joint function and bone metabolic markers (BMMs).

Methods: Admitted from July 2019 to July 2021, 126 patients with OP + KOA were selected, including 76 cases (observation group) treated with GlcN plus SH and 50 cases (control group) given GlcN alone.

Identification of phenolics from miracle berry () leaf extract and its antiangiogenesis and anticancer activities.

Miracle berry is well-known for its ability to convert sour foods to sweet. In this study, the secondary metabolites of miracle berry leaves (MBL) were identified by UPLC-DAD-MS, and its antiangiogenesis and anticancer activities were evaluated by using a zebrafish model and the MCF-7 xenograft mouse model, respectively. The result showed that 18 phenolic compounds were identified in MBL extract, and dominated by the derivatives of quercetin and myricetin.

[Multiplex PCR method for simultaneous identification of Xanthii Fructus and its adulterants].

The purpose of this study is to establish a molecular method to identify Xanthii Fructus and two adulterants, the fruits of Xanthium mongolicum and X. italicum. Xanthii Fructus is the fruit of X.

Multifunctional Zn-Ln (Ln = Eu and Tb) heterometallic metal-organic frameworks with highly efficient I capture, dye adsorption, luminescence sensing and white-light emission.

A new family of isostructural 3d-4f heterometallic metal-organic frameworks (HMOFs), [ZnEuTb(TZI)(DMA)(HO)]·4DMA [x = 0 (1), 0.3 (2), 0.6 (3), 0.

Synthesis With Guaranteed Cost and Less Human Intervention for Human-in-the-Loop Control Systems.

This article studies the problem of synthesis with guaranteed cost and less human intervention for linear human-in-the-loop (HiTL) control systems. Initially, the human behaviors are modeled via a hidden controlled Markov process, which not only considers the inference's stochasticity and observation's uncertainty of the human internal state but also takes the control input to human into account. Then, to integrate both models of human and machine as well as their interaction, a hidden controlled Markov jump system (HCMJS) is constructed.

Hexokinase II Inhibitory Effect of Secondary Metabolites Derived from a Streptomyces sp. Associated with Mud Dauber Wasp.

Insect-microbial symbioses have vast biochemical diversity, which is beneficial to produce bioactive secondary metabolites. In this study, chemical examination of a Streptomyces sp. associated with a mud dauber wasp led to the isolation of fourteen compounds.

[Progress in the mechanisms of neural modulation of innate immunity in Caenorhabditis elegans].

The innate immune system is an important defense barrier against invasive microbial threats in plants and animals. The nematode worm Caenorhabditis elegans is a classic model to investigate the genetic and cellular mechanisms of the interaction between host and pathogens. In the past years, many studies have elucidated the machinery of host-pathogen interactions using C.

[Growth adaptability of Zostera marina at different habitats of the Swan Lake in Rongcheng, China].

Eelgrass (Zostera marina), a seagrass species widely distributed in the coastal regions of northern hemisphere, has suffered with a great decline due to a variety of anthropogenic and environmental stresses. In order to examine the adaptability of eelgrass to different environmental stresses, studies on the morphology and reproductive capacity of eelgrass had been carried out monthly from November 2014 to October 2015 at four different habitats of the Swan Lake, including patch area inintertidal area and subtidal area, eelgrass meadow edge, and eelgrass meadow area. The results showed significant spatio-temporal variations in the morphological parameters and branch frequency of eelgrass shoots at different habitats of the Swan Lake.

[Application of species distribution models in the prediction of marine potential habitat: A review].

The relationship between the distribution of halobios and environmental variables has been a focus of present research in marine ecology. Species distribution models (SDMs) have been widely employed to predict the distribution patterns and potential habitat suitability assessments of marine species and provide an efficient approach for marine biodiversity conservation, invasive species prevention, and fishery management. SDMs mainly include habitat suitability index models, mechanism models, and statistical models.

[Species composition and distribution patterns of ichthyoplankton within and outside artificial reefs in Qingshan Bay, Qingdao, China].

To assess the fish attraction and shelter effects of the artificial reefs in Qingshan Bay of Qingdao, the species composition and distribution patterns of ichthyoplankton in artificial reefs were investigated using vertical and horizontal tows in 2014 and 2015. In total, 7306 fish eggs and 52 fish larvae, belonging to 4 orders, 9 families, 11 genera and 12 species, were collected during 7 cruises in spring, summer and autumn of 2014. In 2015, 10373 eggs and 159 fish larvae, belonging to 6 orders, 11 families, 14 genera and 15 species, were collected in the same period as in 2014.

[Feeding ecology of Conger myriaster and structure of the food webs in artificial reef zone, Laoshan Bay, China].

Based on cage net and longline fishing surveys in Laoshan Bay, China, from April 2015 to January 2017, a total of 279 Conger myriaster stomach samples were analyzed to study their feeding ecology, including diet composition, feeding type, feeding grade, feeding intensity, trophic niche, and trophic level. The stomach content analysis of nine key fish species (including Sebastes schlegelii, Hexagrammos otakii, Hexagrammos agrammus, Sebastiscus mamoratus, Lateolabrax maculatus, etc.), were conducted to examine the food webs in artificial reef zone.

Four 3D coordination polymers based on layers with single - carboxylate bridges: synthesis, structures, and magnetic properties.

Four novel coordination polymers (CPs) based on a new 4-(3,5-dicarboxylphenyl) picolinic acid ligands (HL), [M(L)(HO)]·4HO (M = Mn, 1; Co, 2; Ni, 3, CoNi, 4), have been hydrothermally synthesized, and structurally and magnetically characterized. In these isomorphous CPs, octahedrally coordinated metal ions are linked by the single - carboxylate bridge (μ-COO) to give linear trinuclear motifs. The motifs are connected through the other single - carboxylate bridge (μ-COO) to give a 2D (4,4) layer, and the layers are interlinked by the L ligands into 3D frameworks.

2D carboxylate-bridged Ln coordination polymers: displaying slow magnetic relaxation and luminescence properties in the detection of Fe, CrO and nitrobenzene.

Eight new 2D isostructural lanthanide coordination polymers (Ln-CPs), [Ln(HL)(HO)(NO)]·NO (1-Ln), Ln = Nd, Sm, Eu, Gd, Tb, Dy, Ho, and Yb ions, HL = 4-(3,5-dicarboxylphenyl)-2-methylpyridine, were synthesized by using solvothermal methods and studied by structural analyses, magnetic analyses and luminescent probes. Crystallographic studies revealed that these compounds are 2D frameworks in which dinuclear units with double μ-syn,syn-carboxylate bridges are interlinked by single μ-trans,trans-carboxylate bridges from organic spacers. The layers are further stabilized and combined into 3D architectures through intra- and interlayer ππ stacking interactions and hydrogen bonding, respectively.

Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

Background: Retinal ischemia is a retinal disorder related to retinal vascular occlusion, glaucoma, diabetic retinopathy and age-related macular degeneration. The study aimed to evaluate the protective effects and underlying mechanisms of Chi-Ju-Di-Huang-Wan (CJDHW) against retinal ischemia in rats.

Methods: High intraocular pressure (HIOP)-induced retinal ischemia was established in Wistar rats by raising their intraocular pressure to 120 mmHg for 60 min with in an eye whose anterior chamber was cannulated with a 30-guage needle adapted to a normal saline bottle through an intravenous line.

Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens.

The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells.

A new cube-based dodecanuclear cobalt(ii) cluster with azide and tetrazolate ligands exhibiting ferromagnetic ordering.

A new cubane-based Co(ii) compound, [Co(12)(5-PT)(6)(CO(3))(N(3))2(O)(4)(CH(3)O)(6)(H(2)O)(18)] (1) (5-HPT = 5-phenyl-1H-tetrazole), was synthesized by reaction of 5-HPT, CoCl(2)·6H(2)O and NaN(3) in a methanol solution. In 1, a Co(12) cluster based on three identical Co(4)O(2)(ON)(2) cubane units bridged by a μ(6)-carbonate ligand is linked by π-π stacking interactions between phenylene groups, forming a 3D supramolecular structure. Magnetic investigations indicate that compound 1 exhibits long-range ferromagnetic ordering at about 9 K.

3D Co(II) coordination polymer with ferrimagnetic-like layers based on azide and tetrazolate bridges showing slow magnetic dynamics.

A novel 3D Co(II) coordination polymer, [Co6(4-ptz)6(N3)6]n (1) (4-ptz = 4-(pyridinyl)tetrazole), was synthesized by in situ hydrothermal reaction and characterized magnetically. Compound 1 consists of ferrimagnetic-like Co(II) layers with the title heterobridges and exhibits the spin-glass slow dynamics. The study on spin-glass behavior in mixed azide and tetrazolate bridged compounds is the first example.

Suppression of inflammatory response by flurbiprofen following focal cerebral ischemia involves the NF-κB signaling pathway.

Some studies of animal models of middle cerebral artery occlusion indicate that inflammation plays a key role in the pathogenesis of cerebral ischemia and secondary damage. Flurbiprofen has been suggested to alleviate cerebral ischemia/reperfusion injury in both focal and global cerebral ischemia models, but the mechanisms underlying the protective action are still incompletely understood. In this study we want to investigate the protective effect of flurbiprofen after transient middle cerebral artery occlusion (MCAO) in rats and the role of the NF-κB signaling pathway on this neuroprotective effect.

[Complete genomic analysis of a novel infectious bronchitis virus isolate].

The genome of CK/CH/SD09/005, an isolate of infectious bronchitis virus (IBV), was characterized to enable the further understanding of the epidemiology and evolution of IBV in China. Twenty-five pairs of primers were designed to amplify the full-length genome of CK/CH/SD09/005. The nucleotide sequence of CK/CH/SD09/005 was compared with reference IBV strains retrieved from GenBank.

Elevated expression of nuclear protein kinase CK2α as a poor prognosis indicator in lymph node cancerous metastases of human thyroid cancers.

Aim: To investigate the expression of protein kinase CK2α (CK2α) in human thyroid disease and its relationship with thyroid cancer metastasis.

Materials And Methods: Using immunohistochemistry we measured the expression of CK2α in 76 benign and malignant human thyroid cancer tissues, including 10 pairs of papillary carcinoma tissues with or without lymph node cancerous metastasis and similarly 10 pairs of lymph nodes.

Results: The expression of CK2α was found to be higher in thyroid carcinoma cases (papillary carcinoma, follicular carcinoma, anaplastic carcinoma and medullary carcinoma) than in ones such as chronic lymphocytic thyroiditis, nodular goiter and adenoma.