Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology.

Background: Anoxia is characterized by changes in the morphology, metabolism, and function of tissues and organs due to insufficient oxygen supply or oxygen dysfunction. Gentiana straminea Maxim (G.s Maxim) is a traditional Tibetan medicine.

Separation and identification of an oligomeric light stabilizer Chimassorb 944 by gradient elution chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry.

A non-aqueous reversed-phase high-performance liquid chromatographic (HPLC) method has been developed to separate a light stabilizer Chimassorb 944 into individual oligomers, which are further identified using pre-column fluorescent derivatization and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Consistent with previous studies, we find that the Chimassorb 944 product is a complex mixture consisting of a homologous series with the amine end groups and the number of repeat units (n) span from 1 to 26. In addition to the dominant linear species, cyclic oligomers are present at relatively high levels in the low-mass range.